Probe design

Ebook "Luminescent and photoactive transition metal complexes as biomolecular probes and cellular reagents" starts with a chapter contributed by Professor Alexis D. Ostrowski and Professor Peter C. Ford on the photochemical delivery of the gastrotransmitters nitric oxide and carbon monoxide. The emphasis is on the molecular design and photochemical properties of transition metal complexes that serve as photoactivated nitric oxide releasing moieties (NORMs) and carbon monoxide releasing moieties (CORMs).

266p tudohanhtau1006 29-03-2024 4 1   Download

Polygenic risk scores (PRS), which ofer information about genomic risk for common diseases, have been proposed for clinical implementation. The ways in which PRS information may infuence a patient’s health trajectory depend on how both the patient and their primary care provider (PCP) interpret and act on PRS information. We aimed to probe patient and PCP responses to PRS clinical reporting choices.

16p viellison 28-03-2024 3 2   Download

Ebook "Nano-bio probe design and its application for biochemical analysis" eview the concept of nano-bio probe design for biochemical analysis on the basis of their recent published works. A unique biochemical analysis technology based on fluorescence polarization enhanced by nanoparticles is described here with successful applications in environmental monitoring, rapid and sensitive sensing protease activity and high-throughput screening of inhibitors.

83p coduathanh1122 27-03-2024 1 1   Download

Ebook "Fluorescence in bio-inspired nanotechnology: First as probe, then as function" describes the evolving field of DNA nanotechnology in a lucid and easily accessible way. A central theme in the thesis is how biological structures and mechanisms constitute a basis for the design of novel technologies. Hannestad discusses how self-assembled, nanometer-scale DNA constructs can be functionalized using fluorescent labeling.

127p coduathanh1122 27-03-2024 3 1   Download

Ebook "Energy-dispersive X-ray analysis in the electron microscope" provides an in-depth description of x-ray microanalysis in the electron microscope. It is sufficiently detailed to ensure that novices will understand the nuances of high-quality EDX analysis. Includes information about hardware design as well as the physics of x-ray generation, absorption and detection, and most post-detection data processing. Details on electron optics and electron probe formation allow the novice to make sensible adjustments to the electron microscope in order to set up a system which optimises analysis.

163p coduathanh1122 27-03-2024 3 1   Download

Part 1 book "PCR primer design" includes content: Fast masking of repeated primer binding sites in eukaryotic genomes, design of primers and probes for quantitative real time PCR methods, low concentration initiator primers improve the amplification of gene targets with high sequence variability, degenerate primer design for highly variable genomes,....and other contents.

115p oursky06 17-10-2023 3 1   Download

Chapter 10 - Storage and file structure. In this chapter, we probe below the higher levels as we describe various methods for implementing the data models and languages presented in preceding chapters. We start with characteristics of the underlying storage media, such as disk and tape systems. We then define various data structures that allow fast access to data.

59p haojiubujain04 27-07-2023 6 4   Download

Illumina DNA methylation microarrays enable epigenome-wide analysis vastly used for the discovery of novel DNA methylation variation in health and disease. However, the microarrays’ probe design cannot fully consider the vast human genetic diversity, leading to genetic artifacts.

29p viarchimedes 26-01-2022 10 0   Download

Pooled CRISPR screens are a powerful tool to probe genotype-phenotype relationships at genome-wide scale. However, criteria for optimal design are missing, and it remains unclear how experimental parameters affect results. Here, we report that random decreases in gRNA abundance are more likely than increases due to bottle-neck effects during the cell proliferation phase. Failure to consider this asymmetry leads to loss of detection power.

13p viarchimedes 26-01-2022 5 0   Download

The study assessed the influence of precipitation and soil-type distribution on uptake of small grains in Mberengwa and Zvishavane districts in Zimbabwe. A mixed methods research design was adopted in this study and both qualitative and quantitative research approaches were employed to probe data.

11p thebadguys 15-01-2022 10 0   Download

Reusability of two heterogeneous catalysts in ultrasound (US) assisted biodiesel production was investigated in comparison to each other. An ultrasound (US) generator (200 W, 20 kHz) equipped with a horn type probe (19 mm) was used. Regeneration experiments were planned according to second order central composite design (CCD) method. After the eighth use of the catalysts, biodiesel yield decreased from 99.1% to 90.4% for calcined calcite (CaO) and from 98.8% to 89.8% for calcined dolomite (CaO.MgO).

6p tudichquannguyet 29-11-2021 8 1   Download

Since the introduction of microarrays in 1995, researchers world-wide have used both commercial and custom-designed microarrays for understanding differential expression of transcribed genes. Public databases such as ArrayExpress and the Gene Expression Omnibus (GEO) have made millions of samples readily available. One main drawback to microarray data analysis involves the selection of probes to represent a specific transcript of interest, particularly in light of the fact that transcript-specific knowledge (notably alternative splicing) is dynamic in nature.

10p vilarryellison 29-10-2021 12 1   Download

The rat genome was sequenced in 2004 with the aim to improve human health altered by disease and environmental influences through gene discovery and animal model validation. Here, we report development and testing of a probe set for whole exome sequencing (WES) to detect sequence variants in exons and UTRs of the rat genome.

14p vibeauty 23-10-2021 10 1   Download

Bread wheat has a large complex genome that makes whole genome resequencing costly. Therefore, genome complexity reduction techniques such as sequence capture make re-sequencing cost effective. With a highquality draft wheat genome now available it is possible to design capture probe sets and to use them to accurately genotype and anchor SNPs to the genome.

12p vibeauty 23-10-2021 15 2   Download

RNA-binding proteins (RBPs) play vital roles in many processes in the cell. Different RBPs bind RNA with different sequence and structure specificities. While sequence specificities for a large set of 205 RBPs have been reported through the RNAcompete compendium, structure specificities are known for only a small fraction. The main limitation lies in the design of the RNAcompete technology, which tests RBP binding against unstructured RNA probes, making it difficult to infer structural preferences from these data.

8p vibeauty 23-10-2021 9 0   Download

Reconstructed monomers were used to design a satellite-specific fluorescent in situ hybridization (FISH) probe. The data were also analysed within a phylogenetic framework built using the internal transcribed spacer (ITS) sequences of 45S nuclear ribosomal DNA.

14p vitzuyu2711 29-09-2021 21 1   Download

Structural homology modeling supported by bioinformatics analysis plays a key role in uncovering new molecular interactions within gene regulatory networks. Here, we have applied this powerful approach to analyze the molecular interactions orchestrating death receptor signaling networks.

12p visilicon2711 20-08-2021 16 1   Download

Single nucleotide polymorphism (SNP) array technology has been increasingly used to generate large quantities of SNP data for use in genetic studies. As new arrays are developed to take advantage of new technology and of improved probe design using new genome sequence and panel data, a need to integrate data from different arrays and array platforms has arisen.

18p vilichoo2711 23-06-2021 14 0   Download

This paper presents the design and validation of a neutron survey meter. The meter consists of a PRESCILA neutron probe (with good sensitivity, directional response, gamma rejection, and enhanced high-energy response to 20 MeV) and an electrometer developed at Non-Destructive Evaluation center. The homogeneity response of the PRESCILA neutron probe was investigated as a function of distances from the source in order to obtain the appropriate distance for accurate count-rate measurements using the neutron survey meter.

8p trinhthamhodang1218 14-03-2021 12 2   Download

Increasingly, biologists and biochemists use computational tools to design experiments to probe the function of proteins and/or to engineer them for a variety of different purposes. The most effective strategies rely on the knowledge of the three-dimensional structure of the protein of interest.

7p viwyoming2711 16-12-2020 9 2   Download