Producing embryos in vitro

In this research, we have compared the developing embryos in vitro capacity of oocytes collected from slaughtered cows and ones collected by Ovum Pick Up (OPU) from living cows. As the result showed that, by OPU technology, we have collected many high quality oocytes, that increased the developing capacity of the oocytes grown in vitro. Therefore, that increased the percentage fertilization and the developing capacity of the embryos grown in vitro.

5p thienlangso 15-12-2021 17 0   Download

Aberrant DNA methylation patterns of genes required for development are common in in vitro produced embryos. In this regard, we previously identified altered DNA methylation patterns of in vivo developed blastocysts from embryos which spent different stages of development in vitro, indicating carryover effects of suboptimal culture conditions on epigenetic signatures of preimplantation embryos.

19p vibeauty 23-10-2021 6 1   Download

The transcriptional changes around the time of embryonic genome activation in pre-implantation embryos indicate that this process is highly dynamic. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts.

13p vilichoo2711 23-06-2021 11 1   Download

Work in other species suggests that interleukin-6 (IL6) promotes early embryo development. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production.

11p viwyoming2711 16-12-2020 9 0   Download

Embryo rescue is an in vitro technique that has been used to save the hybrid products of fertilization when they might otherwise degenerate. Embryo culture applied to practical problems is a tissue culture technique that has proven to be of greatest value to breeders. One of the main uses of this technique is to produce interspecific and intergeneric hybrids. Post-zygotic barriers such as endosperm abortion and embryo degeneration leading to low fertility have been overcome through the use of embryo culture and several hybrids have been developed in horticultural crops.

9p nguaconbaynhay5 11-05-2020 6 1   Download

In this study, oocytes were recovered from 200 numbers of goats’ ovaries from different slaughterhouses for subsequent use in-vitro maturation, enucleation, reconstruction and embryo culture to produce cloned embryos. The collected oocytes were classified into three categories (Grade A, B and C) based on their appearance of homogenous granular ooplasm and layers of cumulus cells surrounding oocytes under a stereozoom microscope. The average oocyte recovery, In-vitro maturation and the enucleation rates were found to be 3.36 per ovary, 60.37 per cent and 75.52 per cent respectively.

12p angicungduoc4 26-04-2020 14 0   Download

Wheat is the second most produced staple food crop in world. An efficient method for in vitro micro propagation and genetic transformation of wheat are crucial for both basic and applied research. The present study was initiated with a view to develop a regeneration system in 4 genotypes of wheat cultivars HD2888, HD2329, DBW148, and DBW88 as a prerequisite to transformation. The mature embryos were excised from seeds and cultured on MS medium supplemented with high and low concentrations of cytokinins and auxins respectively.

8p nguaconbaynhay4 22-03-2020 16 2   Download

A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators (BAP, NAA, TDZ, Picloram, 2,4-D). Scape explants produced callus only.

7p vibasque27 27-03-2019 19 1   Download

Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: No Peri- and Postnatal Effects on Calves Born After Transfer of in Vitro Produced Embryos Vitrified by the Open Pulled Straw (OPS) Method...

9p hoami_2511 21-10-2011 50 3   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

4p hoami_238 19-09-2011 44 1   Download