Producing escherichia coli isolated

To determine the dissemination of Extended-Spectrum Beta- Lactamase producing Escherichia coli in healthy individuals in a rural community, in Vu Thu district, Thai Binh province, 2016. To identify microbiological characteristics of Extended- Spectrum Beta-Lactamase producing Escherichia coli isolated from healthy individuals in a rural community, in Vu Thu district, Thai Binh province, 2016.

29p gaocaolon7 18-09-2020 14 1   Download

Escherichia coli are important pathogens that cause serious infections in hospitalized patients. This study aimed to investigate the occurrence of β-lactamase genes in twelve ESBL-producing E. coli isolated from patients in the Santa Casa de Misericordia de Sobral-CE, a teaching hospital in Northeastern Brazil, and analyze the antimicrobial susceptibility profile of these microorganisms.

13p cothumenhmong5 17-05-2020 10 2   Download

Cytochrome P450foxy (P450foxy, CYP505) is a fused protein of cytochrome P450 (P450) and its reductase isolated from the fungus Fusarium oxysporum, which catalyzes the subterminal (x-1x-3) hydroxylation of fatty acids. Here, we produced, purified and characterized a fused recombinant protein (rP450foxy) using the Escherichia coli expression system. Purified rP450foxy was catalytically and spectrally indistinguishable from the native protein, but most of the rP450foxy was recovered in the soluble fraction of E. coli cells unlike the membrane-bound native protein....

8p research12 01-06-2013 55 4   Download

Enterobacter cloacae, isolated from the rhizosphere of cucumbers, produces large amounts of indole-3-acetic acid. Indolepyruvate decarboxylase, the key enzyme in the biosynthetic pathway of indole-3-acetic acid, catalyses the formation of indole-3-acetaldehyde and carbon dioxide from indole-3-pyruvic acid. The enzyme requires the cofac-tors thiamine diphosphate andmagnesium ions for catalytic activity. Recombinant indolepyruvate decarboxylase was purified from the host Escherichia coli strain JM109....

10p fptmusic 16-04-2013 84 3   Download

Saccharomyces cerevisiaepossesses two glycogenin isoforms (designated as Glg1p and Glg2p) that both contain a con-served tyrosine residue, Tyr232. However, Glg2p possesses an additional tyrosine residue, Tyr230 and therefore two potential autoglucosylation sites. Glucosylation of Glg2p was studied using both matrix-assisted laser desorption ionization and electrospray quadrupole time of flight mass spectrometry. Glg2p, carrying a C-terminal (His6) tag, was produced inEscherichia coliand purified.

12p awards 05-04-2013 37 4   Download