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Product purification processes
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Ebook "Mammalian cell cultures for biologics manufacturing" are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors.
262p
nhanphanguyet
28-01-2024
4
2
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Part 2 book "Animal cell technology, from biopharmaceuticals to gene therapy" includes content: Bioreactors for animal cells, monitoring and control of cell cultures, animal cell separation, product purification processes, quality control of biotechnological products, regulatory aspects, intellectual property, recombinant therapeutic proteins, monoclonal antibodies, viral vaccines - concepts, principles, and bioprocesses, bioinsecticides, cell therapies and stem cells, gene therapy.
306p
oursky05
20-09-2023
4
2
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The repertoire of complex proteins produced by the host cell during monoclonal antibody (mAb) production has generated a bottleneck in downstream bioprocessing. Low ppm levels of host cell proteins (HCPs) must be achieved atthe downstream purification process stage to generate an end product suitable for use in humans.
11p
viginny
23-12-2022
4
3
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Platform manufacturing processes are widely adopted to simplify and standardize the development and manufacturing of monoclonal antibodies (mAbs). However, there are mAbs that do not conform to a platform design due to instability or other protein properties leading to a negative impact on product quality or process performance (non-platform mAb).
9p
viginny
23-12-2022
7
3
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Current biopharmaceutical production heavily relies on chromatography for protein purification. Recently, research has intensified towards finding suitable solutions to monitoring the chromatographic steps by multivariate spectroscopic sensors.
9p
viginny
23-12-2022
6
2
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Highly purified mineral oils used for the elaboration of pharmaceutical, food and cosmetic products can contain residual mineral oil aromatic hydrocarbons (MOAH). Quantification of the MOAH level as well as detailed characterization of the aromatic species present is important for safety evaluations and for optimization of the purification process.
8p
viginny
23-12-2022
5
3
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Immobilized metal affinity chromatography (IMAC) is a technique primarily used in research and development laboratories to purify proteins containing engineered histidine tags. Although this type of chromatography is commonly used, it can be problematic as differing combinations of resins and metal chelators can result in highly variable chromatographic performance and product quality results.
19p
viginny
23-12-2022
12
3
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Monoclonal antibodies (mAb) are used as therapeutics and for diagnostics of a variety of diseases, and novel antibodies are continuously being developed to find treatments for new diseases. Therefore, the manufacturing process must accommodate a range of mAb characteristics. Acid-sensitive mAbs can severely compromise product purity and yield in the purification process due to the potential formation of aggregates.
8p
viginny
23-12-2022
12
3
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The purification of extracellular vesicles (EVs) remains a major hurdle in the progression of fundamental research and the commercial application of EV-based products. In this study, we evaluated the potential of heparin affinity chromatography (HAC) to purify neural stem cell-derived EVs as part of a multistep process.
16p
viginny
23-12-2022
6
3
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Various high-throughput systems and strategies are employed by the biopharmaceutical industry for early to late-stage process development for biologics manufacturing. The associated increases to experiment productivity and reduction in material consumption makes high throughput tools integral for bioprocess development.
11p
vironald
15-12-2022
6
2
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Many biotherapeutic formats leverage antibody light chain affinity chromatography to enable robust manufacturing processes and to streamline process development. These include multi-specific antibody and antibody fragment platforms which are often designed for specific capture purification methods that can provide efficient removal of commonly expressed product-related impurities.
10p
vironald
15-12-2022
27
2
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Ten Lactobacillus cultures were considered for the study. Out of ten Lactobacillus cultures, V3 produced maximum β-galactosidase activity (3.381 O.D), followed by NK2 and NK9 up to 24h than other cultures during β-galactosidase activity. Extraction of βgalactosidase was carried out by disruption of Lactobacillus cells using sonication at 3, 4 and 5 minute (pulse 30 second on / 10 seconds off and 35 to 55 % amplitudes), lysozymeEDTA (49 mg of lysozyme in 1.
16p
nguathienthan8
20-10-2020
7
1
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The aim of the present study was to optimize a process for production enzyme Laccase via microbial fermentation using peels of Ash Gourd. It is rich in various nutritional substances which are required for culture of microorganisms.. By estimating proximate values of various substances a synthetic media was prepared for Trichoderma Viride. Laccases were collected extra and intracellular level. The enzyme activity, growth was observed which were treated in various conditions and purified via various steps i.e. Ammonium Sulphate and dialysis.
7p
nguaconbaynhay6
24-06-2020
10
0
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The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway. Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.
14p
vihamax2711
21-04-2020
17
1
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The process of amyloid proteins aggregation causes several human neuropathologies. In some cases, e.g. fibrillar deposits of insulin, the problems are generated in the processes of production and purification of protein and in the pump devices or injectable preparations for diabetics.
11p
vienzym2711
03-04-2020
11
1
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Natural precious products such as aroma compounds, essential oils, and bio-activated materials are usually extracted from about 30,000 botanical species. These extracts are often high competitive market due to their small content (less than 1 %) in plants and high purification cost. Thus, development of a modeling for the optimization of the crude oil extraction is highly paid attention. In this work, a modeling of Vietnam lemongrass oil extraction using steam distillation is developed and the optimization of the process parameters is performed using response surface methodology (RSM).
6p
abcxyz123_02
03-03-2020
28
0
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This research focused on technical assessment of GABA production from rice bran through fermentation by Lactobacillus brevis. Influence of operating pressure on separation of GABA by nanofiltration membrane was investigated and 4 bar was suitable for the nanofiltration process. The purification of GABA by nanofiltration with constant feed volume was carried out and purity of GABA reached 4.8 folds, compared to feed, at 5 volumes of added water. At 40 of concentration factor in concentration of GABA solution by nanofiltration with full recycle of retentate, content of GABA reached 49.8 g/L.
7p
chauchaungayxua2
07-01-2020
27
0
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Dehydration-responsive element-binding (DREB) proteins play a critical role in the plant’s droughttolerance mechanism despite their presence in minor amounts in the cell. In this study, a maize-derived transcription factor protein, ZmDREB2.7, was overexpressed in the Escherichia coli strain Rosetta 1. The interested gene conjugating with the thioredoxin gene (TrxA) and his6 tag in the pET-32a vector encoded a 55.7 kDa fusion protein. The optimum condition for inducing the thioredoxin-his6 -ZmDREB2.7 expression was five hours of induction with 0.05 mM IPTG at 300 C. The Tris-HCl 20 mM pH 8.
7p
caygaocaolon1
13-11-2019
11
0
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Zinc scrap is a source of raw material for zinc oxide production. However, to qualify the requirement of raw material for zinc oxide (99.5%) production, refining this source is needed. Many methods are considered such as rectification, chemical method, etc., but difficult to apply on an industrial scale.
8p
vineptune2711
04-11-2019
13
1
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A simple and effective process for the recovery of intracellular poly(3-hydroxybutyrate) (PHB) from a halophilic bacterial strain - Salinivibrio sp. M318 was developed using a chemical digestion method.
8p
viedison2711
03-09-2019
16
0
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