Protein amyloidogenic

The thesis is organised as follows: Chapter 1 comprises the literature review of protein structure, amyloid fibrils and of the effect of nanomaterials on protein aggregation. Chapter 2 reviews the computational methods used to perform simulations and analysis. Chapter 3 comprises my published article on benchmarking forcefields and sampling methods for disordered proteins using amylin as a case study. Chapter 4 comprises my published article on the effect of oxidation on the properties of graphene oxide flakes in solution.

56p runthenight04 02-02-2023 12 3   Download

In spiders soluble proteins are converted to form insoluble silk fibres, stronger than steel. The final fibre product has longbeen the subject of study;however, little is knownabout the conversion process in the silk-producing gland of the spider. Here we describe a study of the conversion of the soluble form of the major spider-silk protein, spidroin, directly extracted from the silk gland, to ab-sheet enriched stateusing circular dichroism(CD) spectroscopy.

5p research12 23-04-2013 33 1   Download

Amyloid deposits with Arg124 mutated TGFBI protein havebeen identified inautosomal dominant blindingcorneal dystrophies.We assessedin vitrothe mechanisms determin-ing TGFBI protein amyloid transformation involving mutations of Arg124.Eight peptides synthesized following the TGFBI protein sequence, centered on codon Arg124 holding the previously reported amyloidogenic mutations and the respective controls were studied.Cys124 andHis124 mutated peptide preparations contained significantly higher amounts of amyloid than the native peptide....

8p tumor12 22-04-2013 41 2   Download

a-lactalbumin (LA) in its molten globule (MG) state at low pH forms amyloid fibrils. Here, we have studied the aggregation propensities of LA derivatives characterized by a single peptide bond fission (1–40⁄41–123, named Th1-LA) or a deletion of a chain segment of 12 amino acid resi-dues located at the level of the b-subdomain of the native protein (1– 40⁄53–123, named desb-LA). We have also compared the early stages of the aggregation process of these LA derivatives with those of intact LA....

13p awards 06-04-2013 38 2   Download

Cleavage of the small amyloidogenic proteinb2-microglobulin after lysine-58 renders it more prone to unfolding and aggregation. This is important for dialysis-related b2 -microglobulin amyloidosis, since elevated levels of cleaved b2-microglobulin may be found in the circulation of dialysis patients.

14p inspiron33 26-03-2013 48 5   Download

The discovery of prion disease and the establishment of the protein only hypothesis of prion propagation raised substantial interest in the class of maladies referred to as conformational diseases. Although significant pro-gress has been made in elucidating the mechanisms of polymerization for several amyloidogenic proteins and peptides linked to conformational dis-

10p galaxyss3 21-03-2013 52 4   Download

Although ADAM10 is a majora-secretase involved in non-amyloidogenic processing of the amyloid precursor protein, several additional substrates have been identified, most of them in vitro. Thus, therapeutical approaches for the prevention of Alzheimer’s disease by upregulation of this metalloproteinase may have severe side effects.

13p vinaphone15 27-02-2013 29 2   Download

It is assumed that protein fibrils manifested in amyloidosis result from an aggregation reaction involving small misfolded protein sequences being in an ‘oligomeric’ or ‘prefibrillar’ state. This review covers recent optical spectroscopic studies of amyloid protein misfolding, oligomerization and amyloid fibril growth.

9p mobifone23 18-01-2013 49 3   Download