Protein expression systems

Ebook "Heterologous gene expression in E. coli: Methods and protocols" herein describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins.

312p dongmelo 27-05-2024 6 2   Download

Ebook "Endocrine FGFs and Klothos" to restore the affinity to FGF receptors in their target organs, the endocrine FGFs have designated the Klotho family of transmembrane proteins as obligate co-receptors. By expressing Klothos in a tissue-specific manner, this unique co-receptor system also enables the endocrine FGFs to specify their target organs among many tissues that express FGF receptors.

250p ladongphongthanh1008 22-04-2024 5 2   Download

Transcriptome analysis of breast cancer discovered distinct disease subtypes of clinical significance. However, it remains a challenge to define disease biology solely based on gene expression because tumor biology is often the result of protein function. Here, we measured global proteome and transcriptome expression in human breast tumors and adjacent non-cancerous tissue and performed an integrated proteotranscriptomic analysis.

14p vibransone 28-03-2024 2 2   Download

Tumour necrosis factor (TNF) superfamily cytokines and their receptors regulate diverse immune system functions through a common set of signalling pathways. Genetic variants in and expression of individual TNF superfamily cytokines, receptors and signalling proteins have been associated with autoimmune and inflammatory diseases, but their interconnected biology has been largely unexplored.

15p vioraclene 31-03-2024 2 2   Download

Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing nonHodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context of the inflammatory response, two SH2 domain-containing protein tyrosine phosphatases (SHPs), namely SHP-1 and SHP-2, serve as negative regulators.

6p viellison 28-03-2024 2 2   Download

Ebook "Proteomics" is to provide the active researcher with an overview of the types of questions being addressed in proteomics studies and the technologies used to address those questions. Key subjects covered in this book include: an assessment of the limitations of this approach and outlines new developments in mass spectrometry that will advance future research;...

136p coduathanh1122 27-03-2024 1 1   Download

Ebook "Systems biology and biotechnology of Escherichia coli" covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism.

470p cotieubac1004 15-03-2024 2 0   Download

SMARCB1, also known as INI1, is a member of a large protein complex involved in chromatin remodeling and thus the regulation of gene expression. It is located on chromosome 22q11.2. SMARCB1 tumors have been found in various locations, including the sinonasal region, gastrointestinal tract, central nervous system (in atypical teratoid and rhabdoid tumors), and perirenal region (in malignant rhabdoid tumors) in both adults and children.

5p vitiki 30-01-2024 8 2   Download

In ebook "Stress-activated protein kinases" leading researchers in the field discuss the state-of-the-art of many aspects of SAPK signalling in various systems from yeast to mammals. These include various chapters on regulatory mechanisms as well as the contribution of the SAPK signalling pathways to processes such as gene expression, metabolism, cell cycle regulation, immune responses and tumorigenesis.

322p tachieuhoa 28-01-2024 4 2   Download

Part 2 book "Milk proteins - From expression to food" includes content: The whey proteins in milk - thermal denaturation, physical interactions, and effects on the functional properties of; effects of drying on milk proteins; changes in milk proteins during storage of dry powders; interactions and functionality of milk proteins in food emulsions; milk protein–polysaccharide interactions; interactions between milk proteins and micronutrients; model food systems and protein functionality; sensory properties of dairy proteins; milk protein gels;.... and other contents.

338p muasambanhan03 02-01-2024 5 3   Download

Ebook "Mechanism of functional expression of the molecular machines" discusses the mechanism of functional expression of a protein or protein complex utilizing the ATP hydrolysis cycle or proton-motive force from a unique point of view focused on the roles of water. A pivotal factor is the entropic force or potential originating from the translational displacement of water molecules coexisting with the molecular machines in the entire system.

77p lamquandat 28-12-2023 2 2   Download

Ebook "Glucose transport: Methods and protocols" explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo.

270p lucchinguyen 28-12-2023 3 2   Download

This study aims to provide a comprehensive overview of the expression of Secreted Frizzled-related Protein 5 (Sfrp5) in adipose tissues and its association with metabolic disorders.

9p vigeorge 06-12-2023 3 1   Download

Part 1 book "Advances in veterinary dermatology" includes content: Epidemiology of human atopic dermatitis – seven areas of notable progress and seven areas of notable ignorance, the genomics revolution - will canine atopic dermatitis be predictable and preventable; characterization of canine filaggrin - gene structure and protein expression in dog skin; innate immune defense system of the skin,... and other contents.

151p oursky10 07-12-2023 5 2   Download

Part 1 book "Animal nutrition" includes content: The animal and its food, carbohydrates, lipids, proteins, nucleic acids and other nitrogenous compounds, vitamins, minerals, enzymes, digestion, metabolism, evaluation of feeds - digestibility, evaluation of foods - energy content of foods and energy partition, evaluation of foods - systems for expressing energy supply and requirements, evaluation of foods - protein.

367p oursky07 24-10-2023 5 2   Download

The ubiquity-proteasome system is an indispensable mechanism for regulating intracellular protein degradation, thereby affecting human antigen processing, signal transduction, and cell cycle regulation. We used bioinformatics database to predict the expression and related roles of all members of the PSMD family in ovarian cancer.

18p visharma 20-10-2023 2 2   Download

Part 2 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Strategies for vaccine design using phage display derived peptides, laboratory scale production of recombinant HAA86 tick protein in pichia pastoris and in escherichia coli system; transient expression of viral proteins in plants using agrobacterium tumefaciens, recombinant botulinum toxoids - a practical guide for production,.... and other contents.

433p oursky05 20-09-2023 2 0   Download

Dihydropyrimidinase-like 3 (DPYSL3) is a cytosolic phosphoprotein expressed in the nervous system and is crucial for neurogenesis. A previous study showed that increased DPYSL3 expression promotes tumour aggressiveness in pancreatic ductal adenocarcinoma, gastric cancer, and colon cancer. However, the role of DPYSL3 in affecting the biological behaviour of urothelial carcinoma (UC) is not yet understood.

17p vioracle 29-09-2023 3 2   Download

Part 1 book "Molecular biology of the cell" includes content: Cells and genomes; cell chemistry and bioenergetics; proteins DNA, chromosomes, and genomes; DNA, replication, repair, and recombination; how cells read the genome - from DNA to protein; control of gene expression; analyzing cells, molecules, and systems; visualizing cells.

599p oursky02 06-08-2023 6 3   Download

This project is focused on studying the biological effects of low-power RF radiation on the expression and function of mechanosensitive ion channel protein (Piezo-1). To evaluate the effects of long-term exposures, cells were exposed at the frequency of 1800 MHz and power of 17 dBm for a period of 2 and 4 hrs using the transverse electromagnetic (TEM) cell exposure system. To study the effects of short-term RF radiation (10 min), exposures emitted from the mobile phone headset at the frequency of 845 MHz and powers of -15 dBm and -5 dBm were applied to cell cultures.

141p runthenight04 02-02-2023 2 2   Download