![](images/graphics/blank.gif)
Protein immobilization
-
Heparin was immobilized on magnetic chitosan particles to be used as a tool for human plasma protein identification. Chitosan was magnetized by co-precipitation with Fe2+/Fe3+ (MAG-CH). Heparin was functionalized with carbodiimide and N-hydroxysuccinimide and covalently linked to MAG-CH (MAG-CH-hep).
9p
viginny
30-12-2022
8
2
Download
-
Immobilized metal affinity chromatography (IMAC) is a technique primarily used in research and development laboratories to purify proteins containing engineered histidine tags. Although this type of chromatography is commonly used, it can be problematic as differing combinations of resins and metal chelators can result in highly variable chromatographic performance and product quality results.
19p
viginny
23-12-2022
12
3
Download
-
Different degrees of protein purity have been observed in immobilized metal affinity chromatography ranging from extremely high purity to moderate and low purity. It has been hypothesized that the host cell protein composition and the metal ligands are factors governing the purity of a protein obtained after immobilized metal affinity chromatography (IMAC).
10p
viginny
23-12-2022
9
3
Download
-
The pH transition method, developed for the determination of the ion-exchange group density on chromatographic stationary phase, was used for the quantification of immobilized protein A. Monolithic epoxy polyHIPE and particulate CNBr-Sepharose supports were used for immobilization.
7p
viginny
23-12-2022
9
3
Download
-
In this study, the DNA fragment encoding for the C-terminus domain of the AopB from Aeromonas hydrophila AH-1 was cloned into pET-M expression vector and expressed in Escherichia coli BL21 (DE3) host cells. The recombinant AopB-C-terminus domain was successfully purified using immobilized nickel affinity chromatography as a soluble form. Crosslinking analysis among AopB-C-terminus molecules in solution showed that this domain existed as a mixture of tetramer, trimer, dimer, and monomer forms.
6p
guitaracoustic02
08-12-2021
18
3
Download
-
An efficient and inexpensive monolithic stationary phase (PHEMA-HA) has been prepared through an easy process comprising addition of humic acid (HA) to a mixture of 2-hydroxyethyl methacrylate (HEMA) and N,N’- methylenebisacrylamide (MBAAm) and subsequent radical-polymerization at –20 ◦ C. The prepared monolithic material was characterized in terms of various techniques and methods such as elemental analysis, FTIR spectroscopy, scanning electron microscopy, mercury porosimetry, hydrolytic stability tests, pHpzc measurements, water-holding capacity, and water permeability.
16p
tudichquannguyet
29-11-2021
10
1
Download
-
The aminated-silica nanoparticles with a positive zeta potential interact with GFP having a negative zeta potential via ionic force. GFP immobilized by the aminated silica nanoparticles greatly enhances (ca. 2 times) the bioluminescence intensity compared to free GFP. The lipase/aminated-silica nanoparticles catalyzed the transesterification reaction of 1-hexanol and ethyl acetate.
7p
spiritedaway36
28-11-2021
6
0
Download
-
The aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH4 OH/H2 O2 /H2 O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS) was used.
11p
tudichquannguyet
29-11-2021
9
1
Download
-
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.
10p
thiencuuchu
27-11-2021
6
1
Download
-
Protein microarrays are a versatile and widely used tool for analyzing complex protein mixtures. Membrane arrays utilize antibodies which are captured on a membrane to specifically immobilize several proteins of interest at once.
7p
vicolorado2711
22-10-2020
12
0
Download
-
A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction.
7p
vijiraiya2711
27-05-2020
9
0
Download
-
Protein–protein interactions are responsible for many biological processes, and the study of how proteins undergo a conformational change induced by other proteins in the immobilized state can help us to understand a protein’s function and behavior, empower the current knowledge on molecular etiology of disease.
8p
vijiraiya2711
27-05-2020
9
0
Download
-
The present work was undertaken to obtain crude Peroxidase extract from radish to characterize it in terms of pH, temperature, number of times use and decolrization of some dyes. The results shows that peroxidase from radish gave highest specific activity 4391 U/mg, and potassium phosphate buffer (0.1 M, pH 7) was best extraction buffer for peroxidase extraction from radish with specific activity 4489 U/mg, while The highest specific activity was measured for crude extract at 1:2 ratio, it was 4479 U/mg protein.
12p
chauchaungayxua5
05-05-2020
6
1
Download
-
L-ornithine (L-Orn), is an intermediate metabolite in the urea cycle that plays a significant role in humans. L-Orn can be obtained from the catalysis of L-arginine (L-Arg) by arginase. The Pichia pastoris expression system offers the possibility of generating a large amount of recombinant protein.
10p
viuchiha2711
21-04-2020
11
1
Download
-
Dehydration-responsive element-binding (DREB) proteins play a critical role in the plant’s droughttolerance mechanism despite their presence in minor amounts in the cell. In this study, a maize-derived transcription factor protein, ZmDREB2.7, was overexpressed in the Escherichia coli strain Rosetta 1. The interested gene conjugating with the thioredoxin gene (TrxA) and his6 tag in the pET-32a vector encoded a 55.7 kDa fusion protein. The optimum condition for inducing the thioredoxin-his6 -ZmDREB2.7 expression was five hours of induction with 0.05 mM IPTG at 300 C. The Tris-HCl 20 mM pH 8.
7p
caygaocaolon1
13-11-2019
11
0
Download
-
Oceans provide enormous and diverse space for marine life. Invertebrates are conspicuous inhabitants in certain zones such as the intertidal; many are soft- bodied, relatively immobile and lack obvious physical defenses. These animals frequently have evolved chemical defenses against predators and overgrowth by fouling organisms. Marine animals may accumulate and use a variety of toxins from prey organisms and from symbiotic microorganisms for their own purposes. Thus, toxic animals are particularly abundant in the oceans.
23p
uocvong01
24-09-2015
62
6
Download
-
The summer/winter difference in plasma volume may be related to the regularly observed summer fall in red blood cell values and total protein. In a fully dehydrated camel with 65% body water, alimentary water has been reported as 12%, which is similar to the figure for tropical cattle. The total water content of the camel is considerably greater in hot dry weather (up to 75%) than in cooler periods (down to 50%), which indicates its physiological water conserving capability.
0p
conduongdinhmenh
07-05-2013
44
9
Download
-
Về công nghệ enzym/protein người ta đã sử dụng thành công kỹ thuật enzym bất động (immobilized ezyme) hoặc tế bào bất động (immobilized cell) để sản xuất ở quy mô công nghiệp các sản phẩm được tạo thành nhờ hoạt động xúc tác của enzym. Nhờ sử dụng công nghệ gen người ta có thể tạo ra khả năng sản sinh một enzym mới nhờ vi khuẩn hoặc nấm men hoặc là nâng cao thêm lên nhiều lần hoạt tính sản sinh enzym của chúng.
76p
trua_nang
22-04-2013
593
203
Download
-
Carboxypeptidases were purified fromguts of larvae of corn earworm (Helicoverpa armigera), a lepidopteran crop pest, by affinity chromatography on immobilized potato carb-oxypeptidase inhibitor, and characterized by N-terminal sequencing. A larval gut cDNA library was screened using probes based on these protein sequences. cDNA HaCA42 encoded a carboxypeptidase with sequence similarity to enzymesofclan MC [Barrett,A.J.,Rawlings,N. D.& Woessner, J. F. (1998)Handbook of Proteolytic Enzymes. Academic Press, London.], but with a novel predicted spe-cificity towards C-terminal acidic residues....
12p
dell39
03-04-2013
41
2
Download
-
Integrins are the major receptor type known to facilitate cell adhesion and lamellipodia formation on extracellular matrix proteins. However, collagen-related peptide and thrombin have recently been shown to mediate platelet lamellipodia formation when presented as immobilized surfaces.
12p
inspiron33
23-03-2013
55
2
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)