Protocols for preparation

The Cisco Certified Internet Expert Security Certification is an increasingly popular internetworking certification and one of the most popular security certifications in the world. Although CCIE certification builds on the foundation you might have established from the Cisco Certified Network Associate (CCNA) and Cisco Certified Network Professional (CCNP) certifications, there is no prerequisite to attempt to gain CCIE certification. However, attaining CCNA and CCNP certifications will help you understand Cisco subjects and testing strategies.

524p longtimenosee08 31-03-2024 3 1   Download

These reactions involved a condensation reaction of aromatic benzaldehydes, 5,5-dimethyl-1,3-cyclohexanedione, and dicyanomethane in a solvent mixture of ethanol and water, resulting in the desired products in moderate to good yields. The procedure is efficient and eco-friendly, and the products can be easily obtained by recrystallisation. Thus, this protocol offers an alternative to existing methods.

12p viellison 28-03-2024 5 1   Download

Ebook "Novel selenium-mediated rearrangements and cyclisations" carefully investigates carbon nucleophiles in selenocyclisations, as well as reaction protocols for performing such reactions catalytically. After a comprehensive introduction to the element selenium, the author goes on to report the synthesis of several substrates for carbocyclisation reactions and the use of selenium reagents for the preparation of dihydronaphthalenes. This thesis outlines some excellent new synthetic routes which will be useful tools for synthetic organic chemistry in the future.

208p coduathanh1122 27-03-2024 3 1   Download

Ebook "Photoelectrochemical water splitting: Standards, experimental methods, and protocols" outlines many of the techniques involved in materials development and characterization for photoelectrochemical (PEC) – for example, proper metrics for describing material performance, how to assemble testing cells and prepare materials for assessment of their properties, and how to perform the experimental measurements needed to achieve reliable results towards better scientific understanding.

130p coduathanh1122 27-03-2024 2 1   Download

Ebook "Immunocytochemical methods and protocols (Second edition)" are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.

447p coduathanh1122 27-03-2024 3 1   Download

Ebook Asterisk the future of telephony (2nd edition): Part 1 includes content: Chapter 1: a telephony revolution, chapter 2: preparing a system for asterisk, chapter 3: installing asterisk, chapter 4: initial configuration of asterisk, chapter 5: dialplan basics, chapter 6: more dialplan concepts, chapter 7: understanding telephony, chapter 8: protocols for VoIP, chapter 9: the asterisk gateway interface (AGI).

260p longtimenosee03 22-02-2024 3 2   Download

Ebook BCMSN exam certification guide (Second edition): Part 2 includes content: Chapter 11 protecting the spanning tree protocol topology, chapter 12 advanced spanning tree protocol, chapter 13 multilayer switching, chapter 14 router redundancy and load balancing, chapter 15 multicast, chapter 16 quality of service overview, chapter 17 diffserv qos configuration, chapter 18 ip telephony, chapter 19 securing switch access, chapter 20 securing with VLANs, chapter 21 scenarios for final preparation.

336p longtimenosee03 22-02-2024 5 2   Download

Part 1 book "Fundamentals of small animal surgery" includes content: Preoperative patient assessment, basic small animal anesthesia, asepsis in small animal surgery, antibiotic use in small animal surgery, basic surgical instruments, pack preparation for sterilization, operating room protocol, surgical attire, scrubbing, gowning, and gloving, surgical preparation and animal positioning, surgical draping, instrument handling.

156p muasambanhan01 11-12-2023 4 2   Download

Delirium is a significant cause of morbidity and mortality among older people admitted to both acute and long-term care facilities (LTCFs). Multicomponent interventions have been shown to reduce delirium incidence in the acute care setting (30–73%) by acting on modifiable risk factors. Little work, however, has focused on using this approach to reduce delirium incidence in LTCFs.

13p vinobelprisen 26-03-2022 16 5   Download

Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins.

8p vigalileogalilei 27-02-2022 11 1   Download

The cumbersome program and the shortage of commercial solution hindered the regular application of regional citrate anticoagulation (RCA). It is urgent to simplify the protocol using only commercial preparations. The aim of this study was to explore the feasibility and efficacy of the modified protocol for continuous venovenous hemofiltration (CVVH) in unselected critically ill patients.

9p vidietmarhopp 29-12-2021 4 0   Download

A highly efficient and environmentally benign protocol for the synthesis of 2-amino-7-hydroxy-4-aryl-4H-chromene-3-carbonitrile derivatives in good to high yields (77%–97%) by one-pot three-component coupling reaction of aromatic aldehydes, malononitrile, and resorcinol under reflux condition was developed in aqueous media using ZnO nanoparticles that were prepared in the presence of mulberry leaf extract under mild conditions.

9p langthannam 29-12-2021 10 1   Download

CeO2 nanoparticles were used as an efficient catalyst for the preparation of cyclic β-aminoesters by threecomponent reaction between primary amines, ethyl acetoacetate, and chalcones in ethanol. Atom economy, low catalyst loading, reusable catalyst, and high yields of products are some of the important features of this protocol.

7p langthannam 29-12-2021 11 0   Download

In the current protocol, the arene diazonium saccharin derivatives were initially produced from various substituted aromatic amines; subsequently, these intermediates were treated with a greener organic iodide for the preparation of the aryl iodide. We tried to choose low-cost, commercially available, biodegradable, recoverable, ecofriendly, and safe reagents and solvents. The arene diazonium saccharin intermediates could be stored in the liquid phase into a refrigerator for a long time with no significant loss activity.

15p tudichquannguyet 29-11-2021 7 0   Download

This work introduces a new additive named 4,4’-trimethylenedipiperidine for the practical and ecofriendly preparation of ethyl 5-amino-7-(4-phenyl)-4,7-dihydro-[1,2,4]triazolo[1,5-a]pyrimidine-6-carboxylate derivatives. This chemical is commercially available and easy to handle. It also possesses a low melting point and a broad liquid range temperature, high thermal stability, and good solubility in water. Based on green chemistry principles, the reaction was performed in a) a mixture of green solvents i.e.

12p tudichquannguyet 29-11-2021 14 1   Download

Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for which a limited number of PCR amplification cycles is preferred (less PCR bias and/or more even coverage).

10p vibeauty 23-10-2021 11 1   Download

This inhibits 3′ adapter ligation and makes library preparation particularly challenging. To reduce bias, the NEBNext kit (New England Biolabs) uses polyethylene glycol (PEG), the NEXTflex V2 kit (BIOO Scientific) uses both randomised adapters and PEG, and the novel SMARTer (Clontech) and CATS (Diagenode) kits avoid ligation altogether. Here we compared these methods with Illumina’s classical TruSeq protocol regarding the detection of normal and 2’ OMe RNAs. In addition, we modified the TruSeq and NEXTflex protocols to identify conditions that improve performance.

16p vibeauty 23-10-2021 8 0   Download

Cell type-specific ribosome-pulldown has become an increasingly popular method for analysis of gene expression. It allows for expression analysis from intact tissues and monitoring of protein synthesis in vivo. However, while its utility has been assessed, technical aspects related to sequencing of these samples, often starting with a smaller amount of RNA, have not been reported. In this study, we evaluated the performance of five library prep protocols for ribosome-associated mRNAs when only 250 pg-4 ng of total RNA are used.

16p vitzuyu2711 29-09-2021 13 1   Download

Circulating microRNAs (miRNAs) are attractive non-invasive biomarkers for a variety of conditions due to their stability and altered pathophysiological expression levels. Reliable detection of global expression profiles is required to maximise miRNA biomarker discovery.

12p visilicon2711 20-08-2021 6 1   Download

The Urea-Hydrogen Peroxide complex (UHP) was synthesized from urea and hydrogen peroxide, characterized by FT-IR. This UHP complex could be employed as an oxidizing agent for metal-free oxidation reaction of disulfides to thiosulfinate compounds. This protocol was carried out under very mild conditions at 0oC in CH3CO2H solvent, was efficient and compatible with a range of alkyl, aryl or allyl disulfides to afford direct access to thiosulfinate compounds in very good yields up to 92% and high selectivities.

8p cothumenhmong11 05-05-2021 12 1   Download