Purification of lipopolysaccharide

The genus Klebsiella contains a number of opportunistic pathogens which cause several serious infections, including pneumonia, bacteremia, and urinary tract infections. Characteristically, Klebsiella spp. produce large mucoid colonies because of the synthesis of large amounts of capsular polysaccharide (CPS). The LPS molecule is a virulence determinant in Klebsiella pneumoniae. In the present work from a pathogenic urine sample Klebsiella pneumoniae was obtained, the lipopolysaccharide extracted and completely purified by gel filtration chromatography.

11p nguathienthan8 20-10-2020 12 1   Download

Lipopolysaccharide (LPS) is the major outer membrane component of the Gram negative bacteria, supporting the structural integrity and protecting the membrane from chemical attack. Lipopolysaccharide is pyrogenic, causes endotoxic shock and induces the clinical manifestations of infections. It has the broad applications in cell stimulation experiments which abstract, the basis for studies on the isolation, purification and characterization of LPS.

7p chauchaungayxua6 26-06-2020 18 1   Download