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Purification of recombin
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Ebook "Sweeteners: Pharmacology, biotechnology, and applications" introduces various substance classes of sweeteners, which are mainly plant-derived, including glycosidic and terpenoid sweeteners, peptidic sweeteners, sweet-tasting proteins and protein-derived sweeteners (e.g. stevioside, sucralose, aspartame, thaumatin, brazzein and many more).
652p
tracanhphuonghoa1007
22-04-2024
4
3
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Part 2 book "Animal cell technology, from biopharmaceuticals to gene therapy" includes content: Bioreactors for animal cells, monitoring and control of cell cultures, animal cell separation, product purification processes, quality control of biotechnological products, regulatory aspects, intellectual property, recombinant therapeutic proteins, monoclonal antibodies, viral vaccines - concepts, principles, and bioprocesses, bioinsecticides, cell therapies and stem cells, gene therapy.
306p
oursky05
20-09-2023
6
2
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Nattokinase (NK) is a fibrinolytic enzyme with the potential for fighting cardiovascular diseases (CVD) thanks to its antithrombotic, antihypertensive, anticoagulant, anti-atherosclerotic, and neuroprotective effects. Nattokinase was first discovered and purified from soybean fermented food by Bacillus subtilis natto.
9p
vimulcahy
18-09-2023
10
4
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Beta-glucosidase (BGL) is an enzyme involved in the degradation of cellulose and plays an essential part in many biological processes. Currently, most BGLs applied in the industry are derived from fungi. Exploring novel BGLs with desired properties is attractive.
9p
vimulcahy
18-09-2023
3
3
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Aeromonas spp. uses T3SS to secrete and transport effector proteins to the host cells. These proteins play a major role in bacteria virulence by interfering with the signaling cascades and disrupting the cytoskeleton structure of the host cell.
6p
vicaptainmarvel
21-04-2023
8
2
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Human interleukin-3 (IL-3) is a hematopoietic growth factor involved in the survival, proliferation and differentiation of multipotent hematopoietic cells. However, recombinant IL-3 is usually expressed as insoluble form (inclusion bodies) in Escherichia coli cells.
8p
vicaptainmarvel
21-04-2023
4
2
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In a previous study, a gene GL0694641 coding for endoglucanase containing 3 domains GH5- CBM72-CBM72 was exploited from metagenomic DNA data of bacteria in Vietnamese goats’ rumen. The gene (eg5) encoding the mature enzyme (without signal peptide coding sequence) was optimized codons, artificially synthesized, and inserted into the pET22b(+) vector at NcoI and XhoI to generate expression vector pET22-eg5 for expression of the gene in Eschrichia coli.
11p
vicaptainmarvel
21-04-2023
5
2
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The poultry industry in Vietnam is vulnerable to many viral diseases. Since antibiotics and vaccination provide inadequate protection, it will be beneficial to have alternative immunostimulants to boost non-specific immunity in poultry. Chicken interferon-alpha (ChIFNα) has been described previously with antiviral activity against many pathogens.
9p
vicaptainmarvel
21-04-2023
4
2
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A high density lipoprotein (HDL)-linked enzyme with antioxidant and antiatherogenic properties, paraoxonase1(PON1), prevents the formation of atherosclerotic lesions in humans. In the present study, a recombinant hPON1 gene was produced using a small ubiquitin-related modifier (SUMO) fusion protein expression system. To that end, the hPON1 gene was amplified from human liver-ready cDNA, cloned into the expression vector pET SUMO, and expressed in Escherichia coli BL21 (DE3).
13p
langthannam
29-12-2021
8
1
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In this study, the DNA fragment encoding for the C-terminus domain of the AopB from Aeromonas hydrophila AH-1 was cloned into pET-M expression vector and expressed in Escherichia coli BL21 (DE3) host cells. The recombinant AopB-C-terminus domain was successfully purified using immobilized nickel affinity chromatography as a soluble form. Crosslinking analysis among AopB-C-terminus molecules in solution showed that this domain existed as a mixture of tetramer, trimer, dimer, and monomer forms.
6p
guitaracoustic02
08-12-2021
18
3
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The recombinant polymerases of human influenza virus A/PR/8/34 (H1N1) (PR8) and avian influenza virus A/Turkey/England/1969 (H3N2) (TE) using anti-FLAG M2 affinity resin. After confirming trimeric complexes, enzymatic properties of recombinant polymerases were characterized, including model viral RNA binding, in vitro transcription assays, and cap-snatching activity upon addition of cap1-70 mer vRNA as substrate. Taken together we conclude that 293T cells are a suitable expression system for sufficient amount isolation of functional recombinant influenza viral polymerases with 95% of purity.
5p
mudbound
10-12-2021
7
2
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The present study describes the simultaneous expression of thermostable industrial alpha (α) and beta (β) amylase enzymes that have been used widely in starch industry. Genomic DNA of Bacillus stearothermophilus DSM 22 strain for α amylase and, Thermoanaerobacterium (Clostridium) thermosulfurogenes DSM 2229 strain for β amylase were used as gene sources. Both genes were ligated into pETDuet-1 expression vector separately and resulting recombinant vectors were transformed into Escherichia coli BL21 competent cells by electroporation.
7p
thiencuuchu
27-11-2021
17
1
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RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G).
9p
thiencuuchu
27-11-2021
18
1
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The σB protein of avian reovirus (ARV) is diagnostically important protein for development of an ARV-specific enzyme-linked immunosorbent assay (ELISA) and for which production of an antigenic recombinant σB protein in abundance is a prerequisite. In the present study, we successfully employed the prokaryotic system for expression of antigenic σB protein of ARV.
8p
angicungduoc8
07-11-2020
17
2
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In this study, the recombinant endoglucanase was purified by his - tag affinity chromatography with differrent processes, such as using phosphate buffer with or without sodium cloride, pretreatment of samples with ammonium sulphate before supplying into affinity column, using various concentration of imidazole for washing.
9p
tamynhan10
24-10-2020
17
1
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This is the first report in Vietnam showing the extraction and purification of the recombinant single chain antibody recognizing antigen of ABO system using E. coli expression system. It can be considered as a reference for further studies to improve the specificity of recombinant antibody antiA-scFv to identify ABO-type blood antigens.
10p
xilank_of
22-07-2020
22
2
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In this study, we cloned the nucleotide sequence coding C-terminal fragment of PMT (tPMT-780), from acid amine 506 to acid amine 1285, into pRSET-A vector and expressed it on Escherichia coli BL21 bacteria.
8p
tomhiddleston
25-05-2020
14
2
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Tomato leaf curl virus (ToLCV) is a major Geminivirus which cause serious loss to tomato production in tropical and subtropical regions of the world. Hence, considered as major constraint to tomato cultivation. In the present study ToLCV coat protein gene was cloned and expressed using recombinant DNA technology approach. The ToLCV infected tomato leaf samples were collected from tomato fields near Krishi Vigyan Kendra, Dharwad. Further, the total DNA from ToLCV infected tomato leaf sample was isolated by following CTAB protocol.
11p
nguathienthan4
18-04-2020
11
2
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Plasmin is a serine protease that plays a critical role in fibrinolysis, which is a process that prevents blood clots from growing and becoming problematic. Recombinant human microplasminogen (rhμPlg) is a derivative of plasmin that solely consists of the catalytic domain of human plasmin and lacks the five kringle domains found in the native protein.
8p
viuchiha2711
21-04-2020
8
1
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Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is impeded by low expression from transfected cell lines and the presence of serum proteins.
11p
viuchiha2711
21-04-2020
15
0
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