Quantitative pcr

We aimed to screen novel gene signatures for ovarian cancer (OC) and explore the role of biomarkers in OC via regulating pyroptosis using bioinformatics analysis. The proliferation, migration, and invasion were determined using cell counting kit and transwell assay. Enzyme-linked immunosorbent assay was applied to measure infammatory factors. The mRNA and protein expression were detected using real-time quantitative PCR and western blot.

15p vishanshan 27-06-2024 1 1   Download

Head and neck cancers, particularly oropharyngeal cancers (OPC), have been increasingly associated with human papillomavirus (HPV) infections, specifically HPV16. The current methods for HPV16 detection primarily rely on p16 staining or PCR techniques.

13p vishanshan 27-06-2024 1 1   Download

Nghiên cứu nhằm khảo sát vị trí thiết kế mồi trên trình tự giàu CpG của Alu để sử dụng cho phản ứng real time PCR đặc hiệu methyl hóa (quantitative Methylation Specific PCR-qMSP) cho phép đánh giá được sự thay đổi methyl hóa Alu ở 25 cặp mẫu mô ung thư/liền kề của bệnh nhân ung thư vú tại Việt Nam.

9p visergeyne 18-06-2024 0 0   Download

In this study, all the expressions of Otubain-1, NF-κB2, SHPs and STATs genes, the concentrations of cytokines IL-1β, IL-6 and TNF-α and clinical features in NHL patients were examined. To the end, gene expression levels of 82 NHL patients and 56 healthy individuals were determined by quantitative real time RT-PCR and secretion of cytokines by ELISA.

10p dianmotminh02 03-05-2024 5 1   Download

The deregulation of microRNA-140 (miRNA-140) has been reported to be involved in the human pathogenesis of Osteoarthritis (OA). However, in Vietnam, to our knowledge, there is a limited study of evaluation of a miRNA-140 expression. The commercial kits for the quantitation of miRNA-140 are more expensive.

7p vibego 02-02-2024 1 0   Download

In this study, we found that LINC00852 was highly expressed in the tissue of prostate cancer using quantitative reverse transcription PCR (qRT-PCR). CCK-8 assay, colony formation experiment, Transwell migration and invasion experiments were performed to prove that the up-regulation of LINC00852 could promote the proliferation, migration and invasion of prostate cancer cells in vitro.

13p vileonardodavinci 23-12-2023 4 2   Download

In recent years, it has been proved that necroptosis plays an important role in the occurrence, development, invasion, metastasis and drug resistance of malignant tumors. Hence, further evaluation and targeting of necroptosis may be of clinical benefit for gynecologic cancers (GCs).

21p vileonardodavinci 23-12-2023 5 3   Download

To investigate the potential role of Long Non-coding RNAs (lncRNAs) in the progression of osteosarcoma. Methods: The candidate lncRNAs were screened with RNA-seq and confirmed with quantitative real-time PCR. Using MTS, transwell assay, and fow cytometric analysis, the effects of overexpressed lnc-SELPLG-2:1 on cell functions were determined.

15p vialfrednobel 23-12-2023 6 3   Download

Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis. All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using quantitative Real-time PCR as a method of detecting the RNA levels.

17p vialfrednobel 23-12-2023 12 2   Download

Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.

134p oursky10 04-12-2023 4 2   Download

Cervical cancer is currently estimated to be the fourth most common cancer among women worldwide and the leading cause of cancer-related deaths in some of the world’s poorest countries. C/EBPβ has tumor suppressor effects because it is necessary for oncogene-induced senescence. However, C/EBPβ also has an oncogenic role.

13p vischultz 20-10-2023 2 1   Download

To unravel how the integrity of nuclear and mitochondrial circulating cell-free DNA (cfDNA) contributes to its plasma quantity in colorectal cancer (CRC) patients. Methods CfDNA from plasma samples of 80 CRC patients stratified by tumour stage and 50 healthy individuals were extracted. Total cfDNA concentration was determined and equal template concentrations (ETC) were analyzed by quantitative real-time PCR (qPCR) resulting in small and long fragments of KRAS, Alu and MTCO3.

14p vischultz 20-10-2023 4 1   Download

Solute carrier family 25 member 32 (SLC25A32) is an important member of SLC25A family and plays a role in folate transport metabolism. However, the mechanism and function of SLC25A32 in the progression of human glioblastoma (GBM) remain unclear.

14p visharma 20-10-2023 6 2   Download

Cell division cycle 6 (CDC6) is a key licensing factor in the assembly of pre-replicative complexes at origins of replication. The role of CDC6 in the pathogenesis of in diffuse larger B-cell lymphoma (DLBCL) remains unknown. We aim to investigate the effects of CDC6 on the proliferation, apoptosis and cell cycle regulation in DLBCL cells, delineate its underlying mechanism, and to correlate CDC6 expression with clinical characteristics and prognosis of patients with DLBCL.

11p visharma 20-10-2023 5 2   Download

Thyroid cancer is the most frequent malignancy of the endocrine system, of which papillary thyroid cancer (PTC) is the predominant form with a rapid increasing incidence worldwide. Rearranged during transfection (RET) fusions are common genetic drivers of PTC and the potent RET inhibitor selpercatinib has been recently approved for treating advanced or metastatic RET fusion-positive thyroid cancer.

9p visharma 20-10-2023 7 2   Download

Part 1 book "PCR primer design" includes content: Fast masking of repeated primer binding sites in eukaryotic genomes, design of primers and probes for quantitative real time PCR methods, low concentration initiator primers improve the amplification of gene targets with high sequence variability, degenerate primer design for highly variable genomes,....and other contents.

115p oursky06 17-10-2023 3 1   Download

Part 1 book "PCR Protocols" includes content: Long range PCR with a DNA polymerase fusion, isolation of genomic insertion sites of proviruses using splinkerette PCR based procedures, lariat dependent nested PCR for flanking sequence determination, a global single-Cell cDNA amplification method for quantitative microarray analysis,...and other contents.

171p oursky06 17-10-2023 5 1   Download

Part 1 book "PCR Technology - Current innovations" includes content: Pre-PCR processing strategies, use of a liposomal internal control vehicle for whole process quality assurance of nucleic acid amplification, using scorpion primers for genotyping, thermocycler calibration and analytical assay validation, dropletization of bioreactions - how single cell quantitative analyses may improve gene measurement and cancer diagnosis,.... and other contents.

192p oursky06 17-10-2023 3 1   Download

Part 2 book "PCR Technology - Current innovations" includes content: Assay design for Real-Time qPCR, primer design for large scale multiplex PCR and arrayed primer extension, hypothesis driven approaches to multivariate analysis of qPCR data, Experiment Design, data management, and univariate statistical analysis of gene expression data obtained by real time quantitative PCR,.... and other contents.

261p oursky06 17-10-2023 3 1   Download

Part 2 book "Quantitative real-time PCR - Methods and protocols" includes content: Real-Time PCR detection of mycoplasma pneumoniae in the diagnosis of community acquired pneumonia, a sensible technique to detect mollicutes impurities in human cells cultured in GMP condition, real time quantification assay to monitor BCR ABl1 transcripts in chronic myeloid leukemia, a reliable assay for rapidly defining transplacental metastasis using quantitative PCR,... and other contents.

129p oursky05 20-09-2023 5 1   Download