Quickly genotype

Forward-time population genetic simulations play a central role in deriving and testing evolutionary hypotheses. Such simulations may be data-intensive, depending on the settings to the various parameters controlling them. In particular, for certain settings, the data footprint may quickly exceed the memory of a single compute node.

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Green gram is a widely cultivated pulse crop rich in protein, essential amino acids and vitamin-B. The quality and quantity of DNA are very important for amplification by PCR. Although quantity of DNA required per reaction in PCR is very low, quality is very crucial. Also, to carry out large number of PCR reactions for genotyping, a good amount of DNA is required. Presence of contaminants like phenols makes it difficult to get good quality DNA from mungbean.

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Genotyping-by-sequencing (GBS), a method to identify genetic variants and quickly genotype samples, reduces genome complexity by using restriction enzymes to divide the genome into fragments whose ends are sequenced on short-read sequencing platforms.

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Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied methods is ISSR marker analysis. ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes.

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Lichen is a combination of two organisms one is alga and another one is fungus. There is symbiotic relationship between alga and fungus. The algal part provides nutrition to the whole organism and the fungus part provides structural support to the organism.

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Clostridium perfringens is currently classified into five types (A, B, C, D, E) based on the different toxins produced. Type A and C are known as the causative agent of enteritis and enterotoxemia in newborn and young piglets with severe intestinal lesions including edema, hemorrhage and necrosis. A multiplex PCR (mPCR) was developed in order to quickly and early determine the presence of genotypes of C.

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Prunus cerasus L. ‘Oblačinska’ sour cherry germplasm was established in vitro directly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS).

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