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RAD sequencing
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Biodiversity assessment approaches based on molecular biology techniques such as metabarcoding, RAD-seq, or SnaPshot sequencing are increasingly applied in assessing marine and aquatic ecosystems. Here we present a new reference database for fsh meta-barcoding based on mitochondrial genes.
12p
vinarcissa
21-03-2023
3
1
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Recombination rate is an essential parameter for many genetic analyses. Recombination rates are highly variable across species, populations, individuals and different genomic regions.
13p
vinarcissa
21-03-2023
3
1
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Premature flowering or bolting is an undesirable characteristic that causes severe sugar yield losses and interferes with harvesting. Vernalization is a prerequisite for the floral induction, achieved by exposure to low temperatures for 10–14 weeks.
7p
vinarcissa
21-03-2023
2
1
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Photobacteriosis is an infectious disease developed by a Gram-negative bacterium Photobacterium damselae subsp. piscicida (Phdp), which may cause high mortalities (90–100%) in sea bream. Selection and breeding for resistance against infectious diseases is a highly valuable tool to help prevent or diminish disease outbreaks, and currently available advanced selection methods with the application of genomic information could improve the response to selection.
14p
vihagrid
30-01-2023
7
3
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Genome scans based on outlier analyses have revolutionized detection of genes involved in adaptive processes, but reports of some forms of selection, such as balancing selection, are still limited. It is unclear whether high throughput genotyping approaches for identification of single nucleotide polymorphisms have sufficient power to detect modes of selection expected to result in reduced genetic differentiation among populations.
21p
vibeauty
23-10-2021
6
1
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Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars. In recent years, however, new strains of the pathogen have emerged that are capable of overcoming host resistance, posing new challenges for disease management. Despite its economic significance, molecular studies of P.
10p
vibeauty
23-10-2021
9
1
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Flavonoids are important components that confer upon tea plants a unique flavour and health functions. However, the traditional breeding method for selecting a cultivar with a high or unique flavonoid content is time consuming and labour intensive.
11p
viseulgi2711
31-08-2021
15
1
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Restriction site-associated DNA sequencing (RADseq) has revolutionized the study of wild organisms by allowing cost-effective genotyping of thousands of loci. However, for species lacking reference genomes, it can be challenging to select the restriction enzyme that offers the best balance between the number of obtained RAD loci and depth of coverage, which is crucial for a successful outcome.
12p
vigiselle2711
30-08-2021
16
1
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Chinese giant salamander Andrias davidianus is an endangered species. The success of artificial breeding provides a useful way to protect this species. However, the method to identify the sex and mechanism of sex determination were unclear which hinder the improvement of the artificial breeding.
8p
visilicon2711
20-08-2021
10
1
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Foxtail millet (Setaria italica) is one of the oldest domesticated crops and has been considered as an ideal model plant for C4 grasses. It has abundant type of anther and hull colors which is not only a most intuitive morphological marker for color selection in seed production, but also has very important biological significance for the study of molecular mechanism of regulating the synthesis and metabolism of flavonoids and lignin.
13p
vijeeni2711
24-07-2021
13
0
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The development of genome-wide genotyping resources has provided terrestrial livestock and crop industries with the unique ability to accurately assess genomic relationships between individuals, uncover the genetic architecture of commercial traits, as well as identify superior individuals for selection based on their specific genetic profile.
16p
vijeeni2711
30-06-2021
13
1
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Tartary buckwheat (Fagopyrum tataricum), an important pseudocereal crop, has high economic value due to its nutritional and medicinal properties. However, dehulling of Tartary buckwheat is difficult owing to its thick and tough hull, which has greatly limited the development of the Tartary buckwheat processing industry.
12p
vilichoo2711
23-06-2021
8
1
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The irruption of Next-generation sequencing (NGS) and restriction site-associated DNA sequencing (RAD-seq) in the last decade has led to the identification of thousands of molecular markers and their genotyping for refined genomic screening.
16p
vilichoo2711
23-06-2021
11
1
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Transient protein-protein interactions (PPIs), which underly most biological processes, are a prime target for therapeutic development. Immense progress has been made towards computational prediction of PPIs using methods such as protein docking and sequence analysis.
12p
vikentucky2711
26-11-2020
8
0
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n reduced representation sequencing, representative portion of genome is sequenced. Modified reduced representation sequencing method, called double digest Restriction Associated DNA sequencing (ddRADseq) uses two restriction enzymes for skipping the random shearing step of RAD Sequencing. Gradually ddRADseq acquired important role in high density SNP discovery and genotyping, in model and non-model organisms with high efficiency and low cost.
7p
caygaocaolon7
18-09-2020
12
0
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Development of a large set of single nucleotide polymorphisms (SNPs) via next-generation sequencing (NGS)-based methods, restriction-site associated DNA sequencing (RAD-seq), and the generation of a highly saturated genetic map help improve fine mapping of quantitative trait loci (QTL).
12p
viharuno2711
24-04-2020
8
1
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Exponential cost reduction in sequencing with advances in Next Generation Sequencing (NGS) technologies has contributed to rapid genotyping technology innovations. Genome Complexity Reduction Methods such as Restriction Associated DNA Sequencing (RAD-seq) and Genotyping-by-Sequencing (GBS) have emerged as a strong genotyping tool capable of identifying, sequencing and genotyping not hundreds but thousands of markers across almost any genome of interest, but also numbers of individuals in a single, simple experiment in a population.
8p
nguaconbaynhay3
07-02-2020
15
0
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