Random coil conformation

Thebform of human cAMP-dependent protein kinase inhibitor (human PKIb), a novel heat-stable protein, was isolated with high yield using a bacterial expression system. Assays of PKI activity demonstrated that purified PKIb inhibits the catalytic subunit of cAMP-dependent protein kinase. FTIR, Raman spectroscopy and CD experiments implied that human PKIbcontained only small amounts of a-helix andb-structures, but large amounts of random coil and turn structures, which may explain its high thermosta-bility.

6p dell39 03-04-2013 46 4   Download