RAPD marker for the assessment

Genetic diversity among 28 sesame (Sesamum indicum L.) varieties was examined at DNA level by means of random amplified polymorphic DNA (RAPD) analysis. Fifteen primers used produced a total of 132 RAPD fragments. Each primer generated 3 to 26 amplified fragments with an average of 8.8 bands per primer. Based on pair-wise comparisons of RAPD amplification products, Nei and Li’s similarity coefficients were computed to assess the associations among the varieties. Pair-wise similarity indices varied from 0.35 to 0.84.

8p cothumenhmong7 09-09-2020 19 2   Download

Assessment of genotype diversity was studied using released and in pipeline genotypes of wheat, of these 22 were released and 14 were in pipeline . Genetic diversity among 36 wheat genotypes was studied using Random amplified polymorphic DNA (RAPD) analysis. 21 RAPD primers (RPI primers) were used for screening 36 wheat genotypes from which 2,868 fragments were amplified. It was observed that 63.3% bands were polymorphic and 36.4% were monomorphic. The percent of polymorphic bands in banding pattern was calculated and it was highest in RPI-22 (81.3%) while lowest was recorded in RPI-2 (33.

8p chauchaungayxua6 26-06-2020 14 3   Download