Rapid in vitro propagation

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants. These were free-disease symptoms that were collected for surface sterilization.

8p vijaychest 24-04-2024 3 2   Download

Green rose (Rose L.) is a beautiful, precious flower that is loved by many people all over the world. However, the source of seedlings of the ornamental flower variety is very limited. This study was conducted to rapidly propagate green roses by tissue culture.

8p vilamborghini 12-10-2022 9 4   Download

Production of bamboo plants by using vegetative propagation is not simple to establish a commercial fields however micropropagation techniques has much ability to achieve the bamboo seedling demands. In vitro cultures of Bambusa balcooa were achieved by using nodal segment as an explant. Eight different combinations of plant growth regulators with MS basal medium were used for shoot induction and multiplication.

7p trinhthamhodang11 27-04-2021 15 2   Download

The basic MS containing 20g/l sucrose, 8g/l agar, TDZ 4μM and NAA 1μM was the most optimum medium for shoot propagation from seedlings and gave the best shoot quality. NAA had little effect on generating roots of Dendrobium chrysotoxum in vitro.

7p trinhthamhodang1218 18-03-2021 8 2   Download

In view of importance of Quince in the state economy and need for its rapid multiplication, the present investigation was laid to develop a commercial micropropagation protocol of Cydonia oblonga mill cv.SKAU-016.

9p caygaocaolon8 07-11-2020 14 2   Download

Phaius tankervilliae (L’Her.) Blume is one of the most attractive and horticulturally valuable ground orchids of the world. Due to illegal collection for trade and consumption and loss of habitat, the population of this species has decreased rapidly at an alarming rate in natural habitats. In vitro seed germination and seedling development of Phaius tankervilliae technique was successfully established...

7p trinhthamhodang7 31-08-2020 16 2   Download

In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture.

9p nguyenxuankha_bevandan 14-08-2020 16 2   Download

The genetic fidelity of in vitro raised gerbera plants multiplied through micropropagation upto fifteen in vitro subcultures was assessed by using intersimple sequence repeat (ISSR) markers. 40 ISSR primers screened, 20 ISSR primers produced a total of 54 ISSRs clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (15) studied. Thus, a total 1094 bands were generated which exhibited homogeneous banding patterns with ISSR markers. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant.

7p quenchua6 15-06-2020 18 2   Download

Gladiolus (Gladiolus hybrida L.) has been found as a potential cut flower cultivated world widely due to its attractive spikes and elegancy. The plant is propagated vegetatively through corms and cormels but more often its cultivation is hindered due to low multiplication rate of its corm and cormels. Gladiolus can grows through underground stems also, but it is more oftenly attacked by soil borne diseases. In vitro propagation techniques, assumes significance, especially for securing rapid multiplication of the novel cultivars using different explants sources and media.

10p angicungduoc5 12-06-2020 8 2   Download

Suitable medium for rooting was MS + 0.5 mg/L IBA with root shoots at 97.33%, average roots at 5.29 roots/shoot after 4 weeks. On organic substrat, 70% coconut fiber and 30% composted cow manure gave the highest survival rate of 91.33%. The plants grew and developed well during the nursery stage.

9p tomhiddleston 25-05-2020 8 0   Download

Theobroma cacao L. is a tropical fruit tree, the seeds of which are used to create chocolate. In vitro somatic embryogenesis (SE) of cacao is a propagation system useful for rapid mass-multiplication to accelerate breeding programs and to provide plants directly to farmers.

18p viminato2711 22-05-2020 18 1   Download

In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively.

18p viminato2711 22-05-2020 11 0   Download

Wheat is the second most produced staple food crop in world. An efficient method for in vitro micro propagation and genetic transformation of wheat are crucial for both basic and applied research. The present study was initiated with a view to develop a regeneration system in 4 genotypes of wheat cultivars HD2888, HD2329, DBW148, and DBW88 as a prerequisite to transformation. The mature embryos were excised from seeds and cultured on MS medium supplemented with high and low concentrations of cytokinins and auxins respectively.

8p nguaconbaynhay4 22-03-2020 16 2   Download

Citrus, one of the most important group of fruit crops around the world, are propagated at large scale with many difficulties. Propagation through seeds is challenging because of Phytophthora foot rot together with recalcitrance of citrus seeds. Vegetative propagation of Citrus species is mainly performed now-a-days by budding on seedling rootstocks. As heavy losses are experienced among the susceptible seedlings due to Phytophthora and Citrus tristeza virus (CTV), the interest in resistant rootstocks has greatly increased.

14p caygaocaolon2 14-03-2020 10 0   Download

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.

9p cothumenhmong1 08-12-2019 18 0   Download

The highest shoot length (13.1 cm) with 100% frequency was achieved on medium containing 1.0 mg/L GA3. In vitro proliferated shoots were then excised from the shoot clumps and transferred to the rooting medium containing different concentrations of indole butyric acid (IBA; 0.5-2.0 mg/L) and indole acetic acid (IAA; 0.5-2.0 mg/L) alone. Among these, the highest root proliferation was obtained in the medium containing 1.5 mg/L IBA.

8p quilen 08-11-2019 12 0   Download

The present study develops a protocol for rapid in vitro micropropagation of a critically endangered and floriculturally most important epiphytic orchid, Dendrobium primulinum Lindl. through the culture of small shoot tip explants (0.3 to 0.5mm) derived from in vitro grown seedlings. The shoot tip explants cultured on solidified Murashige and Skoog (MS) basal medium and MS medium alone or supplemented with combination of various concentrations of growth regulators; α-naphthalene acetic acid (NAA) and 6 benzylaminopurine (BAP), produced shoots and multiple shoots.

5p bichxuan01643027348 07-08-2019 51 1   Download