Read merging

Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. T

17p viarchimedes 26-01-2022 7 0   Download

The problem of de-novo assembly for metagenomes using only long reads is gaining attention. We study whether post-processing metagenomic assemblies with the original input long reads can result in quality improvement. Previous approaches have focused on pre-processing reads and optimizing assemblers.

11p vioklahoma2711 19-11-2020 11 0   Download

Recent advances in next-generation sequencing have revolutionized genomic research. 16S rRNA amplicon sequencing using paired-end sequencing on the MiSeq platform from Illumina, Inc., is being used to characterize the composition and dynamics of extremely complex/diverse microbial communities.

6p vioklahoma2711 19-11-2020 6 0   Download

Next generation sequencing (NGS) technologies enable studies and analyses of the diversity of both T and B cell receptors (TCR and BCR) in human and animal systems to elucidate immune functions in health and disease. Over the last few years, several algorithms and tools have been developed to support respective analyses of raw sequencing data of the immune repertoire.

6p vioklahoma2711 19-11-2020 9 1   Download

Advances in Illumina DNA sequencing technology have produced longer paired-end reads that increasingly have sequence overlaps. These reads can be merged into a single read that spans the full length of the original DNA fragment, allowing for error correction and accurate determination of read coverage.

9p vicoachella2711 27-10-2020 9 0   Download

Illumina sequencing of a marker gene is popular in metagenomic studies. However, Illumina paired-end (PE) reads sometimes cannot be merged into single reads for subsequent analysis. When mergeable PE reads are limited, one can simply use only first reads for taxonomy annotation, but that wastes information in the second reads.

13p vicolorado2711 22-10-2020 11 0   Download

Practitioners of the theory of constraints (TOC) have been waiting for this book for a long time. Many of you reading this now have had substantial exposure to TOC and perhaps even experience with it. Those who have not will benefit by knowing a little background. TOC, as a management approach, has been around for almost two decades, and its creator, Eliyahu M. Goldratt, has been applying the basic principles even longer than that.

335p conrepcon 12-04-2012 94 19   Download

Third generation (3G) networks aim to merge two of the most successful paradigms in communications: cellular networks and the Internet. The IP (Internet Protocol) Multimedia Subsystem (IMS) is the key element in the 3G architecture that makes it possible to provide ubiquitous cellular access to all the services that the Internet provides. Picture yourself accessing your favorite web pages, reading your email, watching a movie, or taking part in a videoconference wherever you are by simply pulling a 3G hand-held device out of your pocket. This is the IMS vision....

4p 0984272663 27-04-2011 76 8   Download