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Real-Time PCR assay
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As clinical signs and symptoms of nasopharyngeal carcinoma (NPC) are often nonspecific, it is difficult to detect in the early stage, leading to high risk of relapse or recurrent metastasis. Plasma cell-free DNA of Epstein-Barr virus (cf-EBV DNA) is markedly associated with the development of undifferentiated NPC, and its level prior and subsequent to treatment is reliable for the screening, diagnosis, monitoring and prognosis of NPC.
5p
visoros
01-11-2023
3
1
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Lower respiratory tract infections are one of the leading causes of morbidity and mortality in children worldwide. Several viruses can cause bronchiolitis. Respiratory syncytial virus (RSV), parainfluenza viruses (PIV), influenza type A, adenovirus and human metapneumovirus (HMPV) are some of the common viruses found in hospital studies. This study aimed to study the molecular diagnosis of HPMV infections by using a realtime, reverse-transcriptase PCR assay and the evaluation of its prevalence among Egyptian infants clinically diagnosed with acute viral bronchiolitis.
8p
kethamoi6
29-06-2020
19
2
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Objectives: To study fluctuation of plasma cell-free Epstein Barr virus DNA at pre-, mid- and post-radiotherapy of nasopharyngeal carcinoma patients. Methods: 21 nasopharyngeal carcinoma patients of stages I - IVB were followed up and the sensitive realtime PCR assay with detection limit of 25 copies/mL was performed for quantification of plasma cell-free Epstein Barr virus DNA.
7p
vixyliton2711
17-04-2019
20
0
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