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Recombinant antigen
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In this study, the hn gene coding for the HN antigen of NDV genotype VIII was artificially synthesized, ligated into donor vector pFastBacHT A and integrated into the baculovirus genome for the purpose of HN expression in Spodoptera frugiperda 9 (Sf9) cells. The recombinant HN protein was successfully expressed in Sf9 cells in the SF900 III medium.
8p
dianmotminh02
03-05-2024
1
1
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The objective of this study is to successfully clone and express P46 of M. hyopneumoniae in Escherichia coli M15 cells to create an initial material source for developing effective solutions for the prevention and treatment of the PEP caused by M. hyopneumoniae bacteria.
13p
viritesh
02-04-2024
3
1
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The aim of this study was to sequence fulllength p72 gene of a field strain of ASFV causing typical ASF in Dong Nai province in 2020 and fuse this p72 gene with VSV G gene to generate a recombinant fusion gene G-p72 that could simultaneously express both proteins and stimulate a better host immune response than p72 expression alone.
10p
vimichaelfaraday
28-12-2023
7
3
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The Tn antigen (CD175) is an O-glycan expressed in various types of human adenocarcinomas, including colorectal cancer (CRC), though prior studies have relied heavily upon poorly characterized in-house generated antibodies and lectins. In this study, we explored Tn expression in CRC using ReBaGs6, a well-characterized recombinant murine antibody with high specificity for clustered Tn antigen.
11p
vileonardodavinci
23-12-2023
3
2
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Part 2 book "Updates on brucellosis" includes content: Blood cultures for the diagnosis of human brucellosis, antibiotic susceptibility testing of brucella species - old and new drugs, recombinant proteins as antigens in serological diagnosis of brucellosis, treatment of brucellosis, treatment of human brucellosis — review of evidence from clinical trials, control of animal brucellosis — the most effective tool to prevent human brucellosis.
144p
oursky09
08-11-2023
4
2
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Breast tumors show heterogeneity containing cancer stem cells as a small subpopulation of a tumor mass. In the present research, our objective was to evaluate the immunogenicity of prepared nanoparticles containing a novel recombinant CD44v (rCD44v) protein in the mouse model.
14p
vischultz
20-10-2023
2
1
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Part 2 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Strategies for vaccine design using phage display derived peptides, laboratory scale production of recombinant HAA86 tick protein in pichia pastoris and in escherichia coli system; transient expression of viral proteins in plants using agrobacterium tumefaciens, recombinant botulinum toxoids - a practical guide for production,.... and other contents.
433p
oursky05
20-09-2023
2
0
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Part 1 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Challenges in veterinary vaccine development and immunization, development of mycoplasma hyopneumoniae recombinant vaccines, computational prediction of immunodominant epitopes on outer membrane protein (OPM) H of pasteurella multocida toward designing of a peptide vaccine; DNA vaccines against maedi–visna virus, detection of avian antigen specific T-cells induced by viral vaccines,.... and other contents.
445p
oursky05
20-09-2023
0
0
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In this study, alkyl hydroperoxide reductase (AhpC) from M. bovis was evaluated as a new candidate vaccine antigen against TB in BALB/c mice model. The ahpC gene was amplified from M.bovis genome, cloned, and expressed in Escherichia coli. Vaccine antigen AhpC was formulated with Montanide ISA 61 VG, an oil-based emulsion adjuvant. Both IgG and IL-12 responses were observed in mice after administering the formulation both as a subunit vaccine alone and also as a booster vaccine for BCG immunization.
14p
lyhuyenthu
31-01-2023
6
2
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Haemonchus contortus (H. contortus) is one of the most important parasites that cause huge economic losses to small ruminant industry worldwide. Effective prognosis and treatment depend upon the early diagnosis of H. contortus infection.
11p
vigalileogalilei
28-02-2022
20
1
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Recombinant Salmonella enterica serotype Choleraesuis (S. Choleraesuis) vaccine vector could be used to deliver heterologous antigens to prevent and control pig diseases. The recombinant vaccine rSC0012(pS-SaoA) conferred high percentage of protection against S. suis or S. Choleraesuis challenge in BALB/C mice.
16p
vigalileogalilei
28-02-2022
19
1
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Since 2011, numerous highly virulent and antigenic variant viral strains have been reported in pigs that were vaccinated against the swine pseudorabies virus. These infections have led to substantial economic losses in the Chinese swine industry.
15p
vidarwin
23-02-2022
22
1
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Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirusassociated diseases (PCVDs/PCVADs), respectively, and resulted in considerable economic loss to the swine industry worldwide.
13p
vigandhi
23-02-2022
12
1
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Brucellosis is an important zoonosis of serious economic and public health consequences affecting livestock and human beings caused by Brucella spp. The available whole cell antigen and smooth lipopolysaccharide (sLPS) based diagnostics show false positive reactions due to cross reactivity.
9p
gaocaolon9
22-12-2020
9
2
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Recombinant human CD45RO antigen was expressed from E. coli BL21 (DE3), purified and analyzed by SDS-PAGE and Western blotting. The purified CD45RO antigen was used to immunize Balb/c mice. Spleen cells from immunized mouse were collected and fused with P3X63Ag8.653 myeloma cells to form hybridoma. Anti-CD45 antibody-secreting capacity of hybridoma clones was evaluated by ELISA assay. Anti-CD45 MAb from the culture supernatant of the chosen hybridoma clone was purified by affinity chromatography. The MAb was characterized the biochemical characteristics and biological activity.
8p
trinhthamhodang9
04-12-2020
12
2
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The σB protein of avian reovirus (ARV) is diagnostically important protein for development of an ARV-specific enzyme-linked immunosorbent assay (ELISA) and for which production of an antigenic recombinant σB protein in abundance is a prerequisite. In the present study, we successfully employed the prokaryotic system for expression of antigenic σB protein of ARV.
8p
angicungduoc8
07-11-2020
17
2
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The genes that produce antibodies and the immune receptors expressed on lymphocytes are not germline encoded; rather, they are somatically generated in each developing lymphocyte by a process called V(D)J recombination, which assembles specific, independent gene segments into mature composite genes. The full set of composite genes in an individual at a single point in time is referred to as the immune repertoire.
10p
vioklahoma2711
19-11-2020
7
1
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T cells and B cells are essential in the adaptive immunity via expressing T cell receptors and immunoglogulins respectively for recognizing antigens. To recognize a wide variety of antigens, a highly diverse repertoire of receptors is generated via complex recombination of the receptor genes.
9p
vioklahoma2711
19-11-2020
9
0
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The aim of this study was to clone major surface antigen (SAG1) gene of T. gondii tachyzoites into pPROEXHTc bacterial expression vector for better expression of a recombinant SAG1 protein for diagnosis of toxoplasmosis by application in ELISA.
7p
quenchua8
29-09-2020
7
1
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Prostate cancer (PCa) is the second leading cause of cancer deaths in men in the United States. The prostate-specific antigen (PSA), often found at high levels in the serum of PCa patients, has been used as a marker for PCa detection and as a target of immunotherapy.
13p
vijennie2711
29-09-2020
15
0
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