Recombinant DNA technology

The book is divided into six sections, highlighting selected methods in protein chemistry, nucleic acids, recombinant DNA technology (including forensic-based methods), antibody-based techniques, microscopy and imaging, and the use of animals in biomedical sciences.

142p vimeyers 29-05-2024 3 2   Download

Ebook "Bioremediation technology: Recent advances" discusses bioremediation technology-based remediation to restore contaminated sites and protect the environment. It studies the opportunities for more efficient biological processes in molecular biology and ecology. Notable accomplishments of these studies include the cleaning up of polluted water and contaminated land. The book includes invited papers by eminent contributors who provide cost-effective bioremediation strategies to immobilize contaminants for cleanup of environment.

391p giangdongdinh 28-05-2024 1 1   Download

Ebook "Plant cell culture protocols (Third edition)" is taught around the world as part of undergraduate and graduate courses in agronomical and biological sciences. However, the apparent simplicity of cell culture technology should not lead to mistakingly consider it as trivial. It would be very diffi cult to understand modern plant biotechnology without in vitro cell cultures and even today, the application of recombinant DNA technology to important crops is hampered by the poor embryogenic or morphogenetic response of in vitro cultured tissues.

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Part 1 book "Reproductive endocrinology" includes content: Introduction to the molecular organization of the endocrine reproductive system; extracellular signaling receptors; the molecules that transmit information into the cell - the intracellular signaling pathways; introduction to molecular biology - structure and function of the macromoleculesof genetic information; regulation of gene expression; molecular basis of abnormal phenotype; recombinant DNA technology,... and other contents.

159p muasambanhan03 25-12-2023 1 1   Download

Part 1 book "Alberts - Essential cell biology" includes content: Cells - the fundamental units of life; chemical components of cells; energy, catalysis, and biosynthesis; protein structure and function; DNA and chromosomes; DNA replication, repair, and recombination; from DNA to protein - how cells read the genome; control of gene expression; how genes and genomes evolve; modern recombinant DNA technology.

383p muasambanhan01 13-12-2023 4 2   Download

Part 2 book "Molecular and cell biology for dummies" includes content: Genetics - from one generation to the next; molecular genetics - reading the book of life, tools of molecular biology - harnessing the power of DNA; the part of tens.

163p oursky06 17-10-2023 5 4   Download

In this study, the DNA fragment encoding for the C-terminus domain of the AopB from Aeromonas hydrophila AH-1 was cloned into pET-M expression vector and expressed in Escherichia coli BL21 (DE3) host cells. The recombinant AopB-C-terminus domain was successfully purified using immobilized nickel affinity chromatography as a soluble form. Crosslinking analysis among AopB-C-terminus molecules in solution showed that this domain existed as a mixture of tetramer, trimer, dimer, and monomer forms.

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Chapter 20 - DNA technology and genomics. This chapter presents the following content: DNA technology makes it possible to clone genes for basic research and commercial applications, restriction enzymes are used to make recombinant DNA, genes can be clones in recombinant DNA vectors,...

113p tradaviahe19 29-03-2021 9 1   Download

Tomato leaf curl virus (ToLCV) is a major Geminivirus which cause serious loss to tomato production in tropical and subtropical regions of the world. Hence, considered as major constraint to tomato cultivation. In the present study ToLCV coat protein gene was cloned and expressed using recombinant DNA technology approach. The ToLCV infected tomato leaf samples were collected from tomato fields near Krishi Vigyan Kendra, Dharwad. Further, the total DNA from ToLCV infected tomato leaf sample was isolated by following CTAB protocol.

11p nguathienthan4 18-04-2020 10 1   Download

Application of transgenic technology for commercial crop production is faced with biosafety issues. These concerns are based on the argument that recombinant DNA-based transgenic technology differs from traditional breeding in that totally new gens using potentially risky technology are transferred between widely unrelated organisms and the location of these genes on the recipient genome is random, unlike when gene transfer take place through conventional breeding.

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Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generating nucleic acids for which no template is available.

13p vihamax2711 21-04-2020 20 2   Download

The main aim of this study is to critically investigate in current up-to-date the technology of rDNA and its biomedical applications as therapeutic proteins. rDNA is the new technology and is a field of molecular biology where scientist reshape the DNA to form synthetic molecular that help to cure many diseases related to human being. A therapeutic proteins are proteins engineered in laboratory for pharmaceutical purpose, making of insulin is one of the most important benefits ever could be achieved by this technology.

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The document highlights the signif- icance of the molecular approaches for all biological processes in both simple and complex cells. The text also incorporates the most recent refer- ences and has been written for students as well as for teachers of molecular biology, molecular genetics, or biochemistry. Invite you to refer.

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Chapter 12 - DNA technology and genomics. The main contents of this chapter include all of the following: DNA technology makes it possible to clone genes for basic research and commercial applications, restriction enzymes are used to make recombinant DNA, genes can be clones in recombinant DNA vectors.

89p shiwo_ding2 03-04-2019 20 1   Download

After completing this chapter, students will be able to: Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology; outline the procedures for cloning a eukaryotic gene in a bacterial plasmid; define and distinguish between genomic libraries using plasmids, phages, and cDNA;...

42p nomoney10 04-05-2017 28 3   Download

(BQ) Part 1 of the document Microbiology demystified presents the following contents: The world of the microorganism, the chemical elements of microorganisms, observing microorganisms, prokaryotic cells and eukaryotic cells, the chemical metabolism, microbial growth and controlling microbial growth, microbial genetics, recombinant DNA technology.

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Chapter 20 introduce to biotechnology. After completing this chapter, students will be able to: Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology; outline the procedures for cloning a eukaryotic gene in a bacterial plasmid; define and distinguish between genomic libraries using plasmids, phages, and cDNA; describe the polymerase chain reaction (PCR) and explain the advantages and limitations of this procedure;...

61p tangtuy02 08-03-2016 37 2   Download

After studying chapter 23 you will be able to: Know the general properties of a plasmid, know the general properties of restriction endonucleases, know the general properties of restriction endonucleases, know why we transform plasmids into bacteria, know how a cDNA Library is constructed, know how to make cDNA, know why we need to make cDNA,....

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Glofish is the first transgenic animal approved to be consumed by human in the USA  The insertion of different constructs of GFP into the fish genomes to give different green colors.The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973.

54p zingzing09 24-04-2013 75 4   Download

Despite massive efforts in drug discovery fueled by combinatory chemistry, recombinant DNA technology, and highthroughput screening, surprisingly few molecules make it through the drug development process. While the reasons are debated, it is certain that many new chemical entities (NCEs) suffer from recurring problems that hinder development— low water solubility, instability, or inadequate pharmacokinetics. An estimated 43% of NCEs are poorly water-soluble.

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