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Recombinant plasmid
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Terpenoid indole alkaloids (TIAs) vincristine and vinblastine are two essential anticancer drugs used in cancer treatments. These compounds are naturally available only in Catharanthus roseus in low accumulation, leading to efforts to upregulate TIAs substances through other expression organisms. The combination of vindoline and catharanthine into α-3∼,4∼- anhydrovinblastine (AVLB) is an important step for the biosynthesis of vinca alkaloids, which serves as a precursor to vinblastine and vincristine.
10p
dianmotminh02
03-05-2024
4
2
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PhzM and PhzS aUe WZR ³cRUe´ eQ]\PeV WhaW aUe QeceVVaU\ fRU cRQYeUViRQ Rf SheQa]iQe-1- carboxylic acid (PCA) into pyocyanin (PYO) phenazine in Gram (-) bacterium Pseudomonas aeruginosa. Apparently, the raise in copy number of their genes could increase the amount of pyocyanin phenazine in the microbe.
8p
vimulcahy
18-09-2023
4
3
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In this study, we evaluated the reliability of genomic DNAs and cloned recombinant plasmids as standard controls for absolute real-time PCR assay. The associated standard curves were constructed and used for the quantification of Lactobacillus acidophilus probiotics.
4p
mudbound
10-12-2021
30
1
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The present study describes the simultaneous expression of thermostable industrial alpha (α) and beta (β) amylase enzymes that have been used widely in starch industry. Genomic DNA of Bacillus stearothermophilus DSM 22 strain for α amylase and, Thermoanaerobacterium (Clostridium) thermosulfurogenes DSM 2229 strain for β amylase were used as gene sources. Both genes were ligated into pETDuet-1 expression vector separately and resulting recombinant vectors were transformed into Escherichia coli BL21 competent cells by electroporation.
7p
thiencuuchu
27-11-2021
17
1
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In the present study, attempts were made to produce biologically active recombinant mouse hephaestin as a secretory form tagged with green fluorescent protein (GFP), Hpsec-GFP. Plasmid expressing Hpsec-GFP was constructed and transfected into COS and CHO cells. The GFP aided the monitoring expression in real time to select the best conditions to maximise expression and provided a tag for purifying and analysing Hpsec-GFP. The protein had detectable oxidase activity as shown by in-gel and solution-based assays.
13p
thiencuuchu
27-11-2021
8
1
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In this study, we cloned the AA13 PMO gene from Neurospora crassa (NCU08746) into a pEX2B vector and transformed Agrobacterium tumefaciens resulting in a recombinant vector, a necessary step for transferring the constructed expression into Aspergillus oryzae using a recently developed method [20].
6p
kethamoi11
01-04-2021
10
1
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In conclusion, the inducer-free expression vectors carrying Pspac promoters allow the constitutive expression of heterologous recombinant proteins at low levels in B. subtilis.
7p
nguaconbaynhay10
20-02-2021
7
1
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To develop a new 18 high-risk human papillomavirus (HR HPV) detection and genotyping assay, which is important to evaluate the risk degree of HR HPV for causing cancers.
9p
vikuala271
13-06-2020
11
1
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The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated.
12p
vihamax2711
21-04-2020
12
0
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Bovine viral diarrhoea virus (BVDV) is the most prevalent infectious disease of cattle. The envelope glycoprotein (E2) gene of an Indian BVDV isolate was already cloned. In this study, recombinant plasmid pcDNA.BVDV.E2 was used to transfect 70-80% HeLa cell, expressed E2 protein which was confirmed by immunoperoxidase test. Rabit anti mouse HRPO conjugate was used in expression analysis and anti BVDV-E2 hyperimmune serum was raised in mice using recombinant plasmid pcDNA.BVDV.E2 for develop specific antibody.
6p
caygaocaolon2
14-03-2020
14
1
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Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in pJET1.
9p
chauchaungayxua3
07-02-2020
14
1
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The use of toxins for cancer therapy has great promise. Gelonin, a potent plant toxin, causes cell death by inactivating the 60S ribosomal subunit. Recently, we developed a novel gene delivery system using biodegradable cationic heparin-polyethyleneimine (HPEI) nanogels. In the current study, the antitumor activity of a recombinant plasmid expressing gelonin (pGelonin) on human ovarian cancer was assessed.
10p
viwashington2711
04-12-2019
11
1
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Recently environmental metagenomics are useful methodology to study microbial diversity in the environment as well as functional metabolic genes. This study was also based on metagenomic method to discover antibiotic resistance genes from aquatic environments. To create a metagenomic library, the environmental DNA was extracted from water and sediment sample of Thi Nghe canal, Ho Chi Minh City. Total DNA then was fragmented by sizes of 1-3 kb and inserted in to pUC19 plasmid. After transformation into E.
8p
nguathienthan1
27-11-2019
15
0
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Lipocalin-2 (LCN2) is a 25-kDa protein in the lipocalin family that shows antibacterial activity. The aim of this study was to clone human LCN2 into the mammalian-specific pIRES2-AcGFP1 vector and to determine the antibacterial activity of the recombinant pIRES2-AcGFP1-hLCN2 plasmid against major bacterial pathogens that cause bovine mastitis.
17p
vikimsa
22-02-2019
12
1
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A gene encoding β-mannanase from Aspergillus niger GIM3.452 was amplified and inserted into a pPIC9K vector. The resulting recombinant plasmid, pPIC9K-MAN, was transformed into Pichia pastoris GS115. One strain (GSKM-1) having the highest β-mannanase activity of 26.6 U/mL was obtained.
8p
vikimsa
22-02-2019
16
1
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In this study, in order to scale up the production of recombinant porcine phosphotyrosine interaction domain containing 1 (pPID1), a pET-28a (+)-pPID1 expression plasmid was constructed and transformed into Escherichia coli Rosetta (DE3). The recombinant pPID1 was then purified and identified by western blotting, and was also analyzed in vitro for its function.
5p
caplock2711
22-02-2019
26
1
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The cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplified and cloned into E. coli/B. thuringiensis shuttle vector pHT315. The recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2.
8p
vivant2711
01-02-2019
12
1
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In this study, a single-copy suppressor screening to seek for a factor(s) that could rescue the drug sensitivity of sgs1Δmus81Δ100N cells was performed and revealed that Flp1, a site-specific recombinase 1 encoded on the 2-micron plasmid was a suppressor. This result suggests a function of Flp1 in coordination with Mus81 and Sgs1 to resolve the recombinant DNA intermediates.
8p
danhvi10
22-11-2018
13
0
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The synthesis of 7-ACA from cephalosporin C (CPC) by a two-step bioconversion using D-amino acid oxidase (DAAO) and glutaryl 7-ACA acylase (GLA) has been effectively and largely applied in pharmaceutical industry. In this study, the gene gla coding for 720-amino acid GLA from plasmid pUC57::gla was analyzed and successfully inserted into vector pET22b(+) to form expression vector pET22b(+)::gla.
9p
cumeo3000
01-08-2018
26
0
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In this study, we proved that Rad52 and Mus81-Mms4 possess a species-specific functional interaction, indicating that Rad52 and Mus81-Mms4 collaborate in processing of homologous recombination intermediates.
12p
tieuthi3006
16-03-2018
58
1
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