Recombinant protein expression
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Ebook "Heterologous gene expression in E. coli: Methods and protocols" herein describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins.
312p dongmelo 27-05-2024 5 2 Download
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In this study, the hn gene coding for the HN antigen of NDV genotype VIII was artificially synthesized, ligated into donor vector pFastBacHT A and integrated into the baculovirus genome for the purpose of HN expression in Spodoptera frugiperda 9 (Sf9) cells. The recombinant HN protein was successfully expressed in Sf9 cells in the SF900 III medium.
8p dianmotminh02 03-05-2024 1 1 Download
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The objective of this study is to successfully clone and express P46 of M. hyopneumoniae in Escherichia coli M15 cells to create an initial material source for developing effective solutions for the prevention and treatment of the PEP caused by M. hyopneumoniae bacteria.
13p viritesh 02-04-2024 3 1 Download
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Familial ovarian cancer (OC) cases not harbouring pathogenic variants in either of the BRCA1 and BRCA2 OC-predisposing genes, which function in homologous recombination (HR) of DNA, could involve pathogenic variants in other DNA repair pathway genes.
26p vibransone 28-03-2024 2 2 Download
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Ebook "Molecular farming in plants: Recent advances and future prospects" with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.
284p coduathanh1122 27-03-2024 5 1 Download
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Part 1 book "Textbook of biochemistry - With clinical correlations" includes content: Eukaryotic cell structure; DNA and RNA - Composition and structure; DNA and RNA - Composition and structure; DNA replication, recombination, and repair; RNA - Transcription and RNA processing; protein synthesis - Translation and posnranslational modifications; recombinant DNA and biotechnology; regulation of gene expression.
368p muasambanhan04 07-01-2024 3 1 Download
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The aim of this study was to sequence fulllength p72 gene of a field strain of ASFV causing typical ASF in Dong Nai province in 2020 and fuse this p72 gene with VSV G gene to generate a recombinant fusion gene G-p72 that could simultaneously express both proteins and stimulate a better host immune response than p72 expression alone.
10p vimichaelfaraday 28-12-2023 7 3 Download
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Ebook "Synthetic biology: Parts, devices and applications" draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity.
414p lucchinguyen 28-12-2023 4 2 Download
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Part 1 book "Alberts - Essential cell biology" includes content: Cells - the fundamental units of life; chemical components of cells; energy, catalysis, and biosynthesis; protein structure and function; DNA and chromosomes; DNA replication, repair, and recombination; from DNA to protein - how cells read the genome; control of gene expression; how genes and genomes evolve; modern recombinant DNA technology.
383p muasambanhan01 13-12-2023 4 2 Download
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Part 3 book "Biochemistry" includes content: The biosynthesis of amino acids, nucleotide biosynthesis, the biosynthesis of membrane lipids and steroids, the integration of metabolism, DNA replication, repair, and recombination, RNA synthesis and processing, protein synthesis, the control of gene expression in prokaryotes, the control of gene expression in eukaryotes.
336p oursky07 24-10-2023 8 3 Download
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Part 2 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Strategies for vaccine design using phage display derived peptides, laboratory scale production of recombinant HAA86 tick protein in pichia pastoris and in escherichia coli system; transient expression of viral proteins in plants using agrobacterium tumefaciens, recombinant botulinum toxoids - a practical guide for production,.... and other contents.
433p oursky05 20-09-2023 1 0 Download
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Part 1 book "Animal cell technology, from biopharmaceuticals to gene therapy" includes content: Introduction to animal cell technology, animal cells - basic concepts, cloning and expression of heterologous proteins in animal cells, cell metabolism and its control in culture, culture media for animal cells, post-translational modification of recombinant proteins, mechanisms of cell proliferation and cell death in animal cell culture in vitro, mathematical models for growth and product synthesis in animal cell culture.
259p oursky05 20-09-2023 6 2 Download
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Part 2 book "Fundamentals of biochemistry, life at the molecular level" includes content: Nucleotide metabolism, nucleic acid structure, DNA replication, repair, and recombination 26 transcription and RNA processing, protein synthesis, regulation of gene expression.
358p oursky05 20-09-2023 3 2 Download
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The majority of autoimmune and allergic diseases are associated with abnormal expression of interleukin (IL)-33, a member of the IL-1 family of cytokines, that function dually as a proinflammatory cytokine and a transcriptional factor.
16p vimulcahy 18-09-2023 4 3 Download
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Part 1 book "Molecular biology of the cell" includes content: Cells and genomes; cell chemistry and bioenergetics; proteins DNA, chromosomes, and genomes; DNA, replication, repair, and recombination; how cells read the genome - from DNA to protein; control of gene expression; analyzing cells, molecules, and systems; visualizing cells.
599p oursky02 06-08-2023 5 2 Download
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Aeromonas spp. uses T3SS to secrete and transport effector proteins to the host cells. These proteins play a major role in bacteria virulence by interfering with the signaling cascades and disrupting the cytoskeleton structure of the host cell.
6p vicaptainmarvel 21-04-2023 8 2 Download
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Human interleukin-3 (IL-3) is a hematopoietic growth factor involved in the survival, proliferation and differentiation of multipotent hematopoietic cells. However, recombinant IL-3 is usually expressed as insoluble form (inclusion bodies) in Escherichia coli cells.
8p vicaptainmarvel 21-04-2023 4 2 Download
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Currently, searching for new protease inhibitors is of interest to many scientists around the world because they play an important role in controlling harmful proteases. As terrestrial bioactive resources are becoming increasingly depleted, studies are aimed at finding other sources, for example, the ocean.
10p vicaptainmarvel 21-04-2023 4 2 Download
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In this study, alkyl hydroperoxide reductase (AhpC) from M. bovis was evaluated as a new candidate vaccine antigen against TB in BALB/c mice model. The ahpC gene was amplified from M.bovis genome, cloned, and expressed in Escherichia coli. Vaccine antigen AhpC was formulated with Montanide ISA 61 VG, an oil-based emulsion adjuvant. Both IgG and IL-12 responses were observed in mice after administering the formulation both as a subunit vaccine alone and also as a booster vaccine for BCG immunization.
14p lyhuyenthu 31-01-2023 6 2 Download
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The concept of the ‘BRCAness’ phenotype implies the properties that some sporadic breast cancers (BC) share with BRCA1/2-mutation carriers with hereditary BC. Breast tumors with BRCAness have deficiencies in homologous recombination repair (HRR), like BRCA1/2-mutation carriers, and consequently could benefit from poly-(ADP)-ribose poly‑ merase (PARP) inhibitors and DNA-damaging chemotherapy.
13p visusanwojcicki 28-06-2022 11 2 Download