Recombinant virus
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In this study, the hn gene coding for the HN antigen of NDV genotype VIII was artificially synthesized, ligated into donor vector pFastBacHT A and integrated into the baculovirus genome for the purpose of HN expression in Spodoptera frugiperda 9 (Sf9) cells. The recombinant HN protein was successfully expressed in Sf9 cells in the SF900 III medium.
8p dianmotminh02 03-05-2024 1 1 Download
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The aim of this study was to sequence fulllength p72 gene of a field strain of ASFV causing typical ASF in Dong Nai province in 2020 and fuse this p72 gene with VSV G gene to generate a recombinant fusion gene G-p72 that could simultaneously express both proteins and stimulate a better host immune response than p72 expression alone.
10p vimichaelfaraday 28-12-2023 7 3 Download
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Part 1 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Challenges in veterinary vaccine development and immunization, development of mycoplasma hyopneumoniae recombinant vaccines, computational prediction of immunodominant epitopes on outer membrane protein (OPM) H of pasteurella multocida toward designing of a peptide vaccine; DNA vaccines against maedi–visna virus, detection of avian antigen specific T-cells induced by viral vaccines,.... and other contents.
445p oursky05 20-09-2023 0 0 Download
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Part 2 book "Vaccine technologies for veterinary viral diseases methods and protocols" includes content: Generation of recombinant modified vaccinia virus ankara encoding VP2, NS1, and VP7 proteins of bluetongue virus, generation of recombinant capripoxvirus vectors for vaccines and gene knockout function studies,...and other contents.
130p oursky05 20-09-2023 3 0 Download
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Aedes aegypti is the primary vector of arthropod-borne viruses and one of the most widespread and invasive mosquito species. Due to the lack of efficient specific drugs or vaccination strategies, vector population control methods, such as the sterile insect technique, are receiving renewed interest.
10p vihagrid 30-01-2023 5 3 Download
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Porcine circovirus type 2 (PCV2) is an economically important pathogen in the swine industry worldwide. Vaccination remains the principal tool to control PCV2-associated diseases (PCVADs). Current vaccines do not eliminate viral shedding in the environment. To enhance the efficacy of PCV2 vaccines, recombinant viruslike particles (VLPs) of PCV2 were generated by fusing a truncated form of flagellin FliC (TFlg: 85-111aa) with the PCV2 capsid protein (Cap).
10p vigalileogalilei 28-02-2022 12 1 Download
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Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious viral disease of swine. At present, there are vaccines for the control of PRRSV infection, but the effect is not satisfactory. The recombination of attenuated vaccines causes significant difficulties with the prevention and control of PRRSV.
7p vigandhi 23-02-2022 14 1 Download
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Since 2011, numerous highly virulent and antigenic variant viral strains have been reported in pigs that were vaccinated against the swine pseudorabies virus. These infections have led to substantial economic losses in the Chinese swine industry.
15p vidarwin 23-02-2022 22 1 Download
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The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice
Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world.
10p vidarwin 23-02-2022 18 1 Download
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In this study, we constructed four recombinant PRV-S bacterial artificial chromosomes (BACs) carrying the same spike (S) expression cassette of a variant porcine epidemic diarrhea virus strain in different noncoding regions (UL11-10, UL35-36, UL46-27 or US2-1) from AH02LA BAC with TK, gE and gI deletion.
10p vidarwin 23-02-2022 14 1 Download
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Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines.
9p vidarwin 23-02-2022 7 1 Download
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Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirusassociated diseases (PCVDs/PCVADs), respectively, and resulted in considerable economic loss to the swine industry worldwide.
13p vigandhi 23-02-2022 12 1 Download
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Porcine deltacoronavirus (PDCoV) is a new pathogenic porcine intestinal coronavirus, which has appeared in many countries since 2012. PDCoV disease caused acute diarrhea, vomiting, dehydration and death in piglets, resulted in significant economic loss to the pig industry.
16p vidarwin 22-02-2022 14 2 Download
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The development of an influenza vaccine for poultry that provides broadly protective immunity against influenza H5Nx viruses is a challenging goal. L. lactis/pNZ8149-HA1-M2 can serve as a promising vaccine candidate in poultry industry for providing protection against H5Nx virus infection in the field application.
12p vidarwin 22-02-2022 12 1 Download
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The recombinant polymerases of human influenza virus A/PR/8/34 (H1N1) (PR8) and avian influenza virus A/Turkey/England/1969 (H3N2) (TE) using anti-FLAG M2 affinity resin. After confirming trimeric complexes, enzymatic properties of recombinant polymerases were characterized, including model viral RNA binding, in vitro transcription assays, and cap-snatching activity upon addition of cap1-70 mer vRNA as substrate. Taken together we conclude that 293T cells are a suitable expression system for sufficient amount isolation of functional recombinant influenza viral polymerases with 95% of purity.
5p mudbound 10-12-2021 7 2 Download
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Intrinsic structural disorder is a common property of many proteins, especially in eukaryotic and virus proteomes. The tendency of some proteins or protein regions to exist in a disordered state usually precludes their structural characterisation and renders them especially difficult for experimental handling after recombinant expression.
9p viseulgi2711 31-08-2021 9 1 Download
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Cotton leaf curl disease (CLCuD), caused by begomoviruses in association with satellite molecules, is a major threat to cotton production causing enormous losses to cotton crop in most of the cotton growing countries including Indian subcontinent.
13p visilicon2711 20-08-2021 5 1 Download
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Several lines of evidence suggest that recombination plays a central role in replication and evolution of herpes simplex virus-1 (HSV-1). G-quadruplex (G4)-motifs have been linked to recombination events in human and microbial genomes, but their role in recombination has not been studied in DNA viruses.
11p visilicon2711 20-08-2021 3 1 Download
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Các bệnh dịch do virus luôn là mối đe dọa thường xuyên và gây thiệt hại nghiêm trọng cho ngành chăn nuôi gà ở Việt Nam. Interferon alpha gà tái tổ hợp (rChIFN-α, recombinant chicken interferon alpha) đã được biểu hiện thành công trên hệ thống pichia pastoris và có hoạt tính kháng virus. Chính vì vậy bài viết này tiến hành xác định hiệu quả của rChIFN-α trên gà 3 tuần tuổi nhiễm virus Gumboro.
11p caygaocaolon9 04-01-2021 32 5 Download
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Many computational tools that detect recombination in viruses are not adapted for the ongoing genomic revolution. A computational tool is needed, that will rapidly scan hundreds/thousands of genomes or sequence fragments and detect candidate recombination events that may later be further analyzed with more sensitive and specialized methods.
8p vioklahoma2711 19-11-2020 8 0 Download