Restricted recombination

The black yeast Hortaea werneckii (Dothideomycetes, Ascomycota) is one of the most extremely halotolerant fungi, capable of growth at NaCl concentrations close to saturation. Although dothideomycetous fungi are typically haploid, the reference H. werneckii strain has a diploid genome consisting of two subgenomes with a high level of heterozygosity.

12p vibeauty 23-10-2021 12 1   Download

Chapter 20 - DNA technology and genomics. This chapter presents the following content: DNA technology makes it possible to clone genes for basic research and commercial applications, restriction enzymes are used to make recombinant DNA, genes can be clones in recombinant DNA vectors,...

113p tradaviahe19 29-03-2021 9 1   Download

Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function at low temperatures.

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The methanol-regulated AOX1 promoter (PAOX1) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of PAOX1 restricts its application, it is necessary to develop a flexible induction system to avoid the problems of methanol without losing the advantages of PAOX1.

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Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in pJET1.

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Cotton, Recombinant inbred lines (RIL), phenotypic (PCV) and There is a limited genetic variability available in genetic resources of upland cotton (Gossypium hirsutum L.), which restricted the breeding activity. The objective of present study was evaluating the genetic variability in RIL population derived through intraspecific hybridization between RHAP 12 and RHAP 15 genotypes. The 222 RIL were evaluated during kharif 2018 at University of Agricultural Sciences Dharwad.

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Streptococcus dysgalactiae is an emerging pathogen of fish. Clinically, infection is characterized by the development of necrotic lesions at the caudal peduncle of infected fishes. The pathogen has been recently isolated from different fish species in many countries. Twenty S. dysgalactiae isolates collected from Japan, Taiwan, Malaysia and Indonesia were molecularly characterized by biased sinusoidal field gel electrophoresis (BSFGE) using SmaI enzyme, and tuf gene sequencing analysis. DNA sequencing of ten S.

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Chapter 12 - DNA technology and genomics. The main contents of this chapter include all of the following: DNA technology makes it possible to clone genes for basic research and commercial applications, restriction enzymes are used to make recombinant DNA, genes can be clones in recombinant DNA vectors.

89p shiwo_ding2 03-04-2019 20 1   Download

After completing this chapter, students will be able to: Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology; outline the procedures for cloning a eukaryotic gene in a bacterial plasmid; define and distinguish between genomic libraries using plasmids, phages, and cDNA;...

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Chapter 20 introduce to biotechnology. After completing this chapter, students will be able to: Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology; outline the procedures for cloning a eukaryotic gene in a bacterial plasmid; define and distinguish between genomic libraries using plasmids, phages, and cDNA; describe the polymerase chain reaction (PCR) and explain the advantages and limitations of this procedure;...

61p tangtuy02 08-03-2016 37 2   Download

After studying chapter 23 you will be able to: Know the general properties of a plasmid, know the general properties of restriction endonucleases, know the general properties of restriction endonucleases, know why we transform plasmids into bacteria, know how a cDNA Library is constructed, know how to make cDNA, know why we need to make cDNA,....

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Yet, not enough countries use these and other effective policy options to prevent death, disease and injury attributable to alcohol consumption. Since 1999, when WHO fi rst began to report on alcohol policies, at least 34 countries have adopted some type of formal policies. Restrictions on alcohol marketing and on drink–driving have increased but, in general, there are no clear trends on most preventive measures.

5p quygia123 01-11-2012 59 1   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides

12p toshiba17 28-10-2011 54 2   Download