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Reverse genetic system
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Thyroid cancer is the most frequent malignancy of the endocrine system, of which papillary thyroid cancer (PTC) is the predominant form with a rapid increasing incidence worldwide. Rearranged during transfection (RET) fusions are common genetic drivers of PTC and the potent RET inhibitor selpercatinib has been recently approved for treating advanced or metastatic RET fusion-positive thyroid cancer.
9p
visharma
20-10-2023
8
2
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(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 1 presents the following content: Chapter 1 : analysis of high-throughput sequencing data; chapter 2: identification of plant micrornas using expressed sequence tag analysis; chapter 3: microarray data analysis; chapter 4: setting up reverse transcription quantitative-pcr experiments; chapter 5: virus-induced gene silencing in nicotiana benthamiana and other plant species; chapter 6: agroinoculation and agroinfiltration: simple tools for complex gene function analyses; chapter 7: full-length cdna overexpressor gene hunting syst...
134p
runordie7
30-08-2022
7
2
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Akabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health. We successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation.
9p
vidarwin
22-02-2022
15
1
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Goldfish is an important model for various areas of research, including neural development and behavior and a species of significant importance in aquaculture, especially as an ornamental species. It has a male heterogametic (XX/XY) sex determination system that relies on both genetic and environmental factors, with high temperatures being able to produce female-to-male sex reversal.
12p
vijeeni2711
30-06-2021
12
1
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In any gene regulatory network (GRN), the complex interactions occurring amongst transcription factors and target genes can be either instantaneous or time-delayed. However, many existing modeling approaches currently applied for inferring GRNs are unable to represent both these interactions simultaneously.
22p
viwyoming2711
16-12-2020
14
1
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In this work we created a simple dynamic model for HIV infection with three parameters: The concentration of HIV virus, the concentration of health and the infected T-cells. Using a ‘therapy starting moment’ and this dynamic model, we investigated the effectiveness of HIV drug treatment.
7p
tamynhan9
02-12-2020
13
3
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With the emerging interest in phytoplankton research, the need to establish genetic tools for the functional characterization of genes is indispensable. The CRISPR/Cas9 system is now well recognized as an efficient and accurate reverse genetic tool for genome editing.
4p
vioklahoma2711
19-11-2020
9
1
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Carica papaya is a trioecious plant species with a genetic sex-determination system defined by sex chromosomes. Under unfavorable environmental conditions male and hermaphrodite exhibit sex-reversal. Previous genomic research revealed few candidate genes for sex differentiation in this species.
22p
viuchiha2711
21-04-2020
12
2
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An improved Reactor Regulating System (RRS) logic architecture, which is combined with genetic algorithm (GA), is implemented in this work. It is devised to provide an optimal solution to the current RRS. The current system works desirably and has contributed to safe and stable nuclear power plant operation. However, during the ascent and descent section of the reactor power, the RRS output reveals a relatively high steady-state error, and the output also carries a considerable level of overshoot.
15p
minhxaminhyeu4
26-06-2019
9
0
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The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions.
9p
danhdanh11
11-01-2019
29
1
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An Adenovirus Vector-Mediated Reverse Genetics System for Influenza A Virus Generation established an alternative reverse genetics system for influenza virus generation by using an adenovirus vector (AdV) which achieves highly efficient gene transfer independent of cell transfection efficiency.
4p
thuyancn
01-06-2015
28
3
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An improved reverse genetics system for influenza A virus generation and its implications for vaccine production present an improved system for the generation of influenza viruses that allows the easy and repro ducible production of vaccine viruses in vero cells. Application of this system may be especially advantageous in situations of outbreaks of highly pathogenic avian influenza viruses.
5p
thuyancn
01-06-2015
28
3
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Glutathione (GSH) plays a key role in defense against oxidative stress. The availability of GSH is ensured in tissues by systems devoted to its mainte-nance in the reduced state and by the flux of GSH and cysteine between sites of biosynthesis and sites of utilization. Little is known about the effect of oxidative stress on the distribution of low-molecular-mass thiols and their exchange rate between tissues.
13p
vinaphone15
25-02-2013
45
3
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The treatment of AIDS remains a serious challenge owing to high genetic variation of Human Immunodeficiency Virus type 1 (HIV-1). The use of different antiretroviral drugs (ARV) is significantly limited by severe side-effects that further compromise the quality of life of the AIDS patient. In the present study, we have evaluated a liposome system for the delivery of nevirapine, a hydrophobic non-nucleoside reverse transcriptase inhibitor. Liposomes were prepared from egg phospholipids using thin film hydration.
9p
toshiba23
18-11-2011
45
3
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