Selective chemical labeling

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Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targets

Conventional techniques for single-base resolution mapping of epigenetic modifications of DNA such as 5-hydroxymethylcytosine (5hmC) rely on the sequencing of bisulfite-modified DNA. Here we present an alternative approach called SCL-exo which combines selective chemical labeling (SCL) of 5hmC in genomic DNA with exonuclease (exo) digestion of the bead-trapped modified DNA molecules.

12p viaristotle 29-01-2022 15 0 Download

Application of image-based high content analysis for the screening of bioactive natural-products

The phenotypic changes in target cell induced by an exogenous agent, e.g. biologically active chemical, can be detected by labelling selected organelles with fluorescent dyes and observed under the high-throughput microscope. The changes of known biological targets (e.g. nucleus, proteins, mitochondria, microtubules, etc.) induced by a compound of interest allow predicting its mechanisms of action. High-throughput microscopy with capability of multiparametric imaging in combination with automated image-analysis is referred to as highcontent analysis or high-content screening (HCS).

12p abcxyz123_02 03-03-2020 12 1 Download