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Selective PCR
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Accurate assessment of axillary status after neoadjuvant therapy for breast cancer patients with axil‑ lary lymph node metastasis is important for the selection of appropriate subsequent axillary treatment decisions. Our objectives were to accurately predict whether the breast cancer patients with axillary lymph node metastases could achieve axillary pathological complete response (pCR).
13p
vishanshan
27-06-2024
2
1
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Part 1 book "Quantitative real-time PCR - Methods and protocols" includes content: Minimum information necessary for quantitative real; time PCR experiments; selection of reliable reference genes for RT-QPCR analysis; introduction to digital PCR; mediator probe PCR - detection of real time PCR by label; free probes and a universal fluorogenic reporter,... and other contents.
104p
oursky05
20-09-2023
4
1
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This study aims to compare microRNA expression levels in wt and Bag-1 knockout (KO) MCF-7 breast cancer cells. hsa-miR-429 was selected as a potential miRNA in BAG-1KO MCF-7 cells because of the downregulation both in bioinformatics and validation qRT-PCR assay. According to predicted mRNA targets and functional enrichment analysis the ten hub proteins that are phosphatidylinositol4,5-biphosphate 3-kinase catalytic subunit alpha (PIK3CA), kinase insert domain receptor (KDR), GRB2 associated binding protein 1 (GAB1).
30p
lyhuyenthu
31-01-2023
2
2
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Strawberry (Fragaria x ananassa Duch.) is a favorite fruit of high economic value due to its good taste and high nutrition ingredients. Strawberry is a fruit grown around the world that has distinct genetic structures and indicates diverse levels of precision to different environmental circumstances. Plants respond differently to diverse physiological processes, organizing of biological events, control of hormones, different individuals of the same species, and internal and external factors in developmental stages.
16p
lyhuyenthu
31-01-2023
9
2
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In this study, The QSPR models were built with knotp, SHBa, HOMO, xvpc4, N4, LUMO, ionization potential, dipole, molecular weight, Maxneg and Hf descriptors selected from the descriptor sets.
13p
viaudi
04-08-2022
4
1
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Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative measurement of gene expression. The proper choice of internal standards such as reference genes is crucial for correct data evaluation. In female dogs, as in other species, the reproductive tract is continuously undergoing hormonal and cycle stagedependent morphological changes, which are associated with altered gene expression.
12p
vigandhi
23-02-2022
9
1
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We describe a highly sensitive, quantitative, and inexpensive technique for targeted sequencing of transcript cohorts or genomic regions from thousands of bulk samples or single cells in parallel. Multiplexing is based on a simple method that produces extensive matrices of diverse DNA barcodes attached to invariant primer sets, which are all pre-selected and optimized in silico.
16p
vielonmusk
30-01-2022
21
0
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: The main goal of this work was to relate the germinative ecology and genetic variability of Chaerolhyllum aureum L., an Apiaceae plant with dormant, underdeveloped embryos at the time of seed dispersal. We compared the seed germination physiology traits between a relictic geographically isolated Mediterranean population (Iberian System; central Spain) and a population located in the main, colder Atlantic Alpine core (Pyrenees; northern Spain). We analyzed both populations’ genetic identity in parallel on the basis of ISSR–PCR analyses.
11p
tudichquannguyet
29-11-2021
7
1
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In the present study it was aimed to obtain novel strains of actinomycetes that have the ability to promote plant growth. For this, seven soil samples from the rhizosphere of Humulus lupulus (Pazaryeri, Bilecik) were used and potential isolates were obtained. 16S rRNA genes of 30 isolates were amplified by PCR and sequenced. Eighteen isolates were found to be closely related to Streptomycetes spp. and they were tested for their proteolytic activity, cellulase activity, phosphate solubility, IAA production, biofilm formation, and growth in nitrogen-limited medium.
17p
thiencuuchu
27-11-2021
4
1
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The osteogenic differentiation markers BMP2, BMP4, COL1A1, OPN, OCN and PF4 evaluated using RT-PCR. The tumor dimensions in the hMSCs group were significantly higher than those of the remaining groups (p < 0.01). The number of metastatic foci in the BMP2+hMSCs group was significantly lower than those of the other groups (p < 0.01). The current results showed that the intraperitoneal route could be efficiently used for targeting hMSCs to the tumoral tissues for effective BMP2 delivery.
13p
thiencuuchu
27-11-2021
14
1
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In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D low diet, application of steroids, or a combination of these treatments. Transcriptional studies can be performed by applying quantitative real time PCR (RT-qPCR). RT-qPCR estimates mRNA-levels of target genes in relation to reference genes. A chosen set of reference genes should not show variation under experimental conditions.
9p
vilarryellison
29-10-2021
8
0
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Adenosine-to-inosine (A-to-I) RNA editing is an epigenetic modification catalyzed by adenosine deaminases acting on RNA (ADARs), and is especially prevalent in the brain. We used the highly accurate microfluidics-based multiplex PCR sequencing (mmPCR-seq) technique to assess the effects of development and environmental stress on A-to-I editing at 146 pre-selected, conserved sites in the rat prefrontal cortex and amygdala. Furthermore, we asked whether changes in editing can be observed in offspring of stress-exposed rats. In parallel, we assessed changes in ADARs expression levels.
16p
vilarryellison
29-10-2021
17
0
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Sugarcane (Saccharum L. plant) is an important crop for sugar and bio-energy production around the world. Among sugarcane diseases, smut caused by Sporisorium scitamineum is one of the major fungal diseases causing severe losses to the sugarcane industry. The use of PCR reference genes is essential to the normalization of data on gene expression involving the sugarcane-S. scitamineum interaction system; however, no report that addresses criteria in selecting these reference genes has been published to date.
13p
vibeauty
23-10-2021
9
1
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Target enrichment is a critical component of targeted deep next-generation sequencing for the costeffective and sensitive detection of mutations, which is predominantly performed by either hybrid selection or PCR.
10p
visilicon2711
20-08-2021
19
1
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Reverse transcription quantitative PCR (RT-qPCR) is widely used for gene expression analysis in various organisms. Its accuracy largely relies on the stability of reference genes, making reference gene selection a vital step in RT-qPCR experiments. However, previous studies in mollusks only focused on the reference genes widely used in vertebrates.
12p
visilicon2711
20-08-2021
8
1
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In quantitative real-time polymerase chain reaction (qRT-PCR) experiments, accurate and reliable target gene expression results are dependent on optimal amplification of house-keeping genes (HKGs). RNA-seq technology offers a novel approach to detect new HKGs with improved stability.
16p
vijeeni2711
24-07-2021
5
0
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Before studying gene expression of different organisms, it is important to determine the best reference gene. At present, the most accurate method of detecting gene expression is quantitative real-time PCR (RT-qPCR).
14p
vijichea2711
28-05-2021
7
1
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Numerous models for use in interpreting quantitative PCR (qPCR) data are present in recent literature. The most commonly used models assume the amplification in qPCR is exponential and fit an exponential model with a constant rate of increase to a select part of the curve.
13p
viwyoming2711
16-12-2020
9
0
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The study was done in the Department of Microbiology, at University College of Medical Sciences and Guru Tegh Bahadur Hospital, Delhi from 2011 to 212.A preformatted questionnaire was used for selection of 100 patients with suspected enteric fever to be included in the study.
6p
caygaocaolon8
07-11-2020
8
2
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PRISE2 is a new software tool for designing sequence-selective PCR primers and probes. To achieve high level of selectivity, PRISE2 allows the user to specify a collection of target sequences that the primers are supposed to amplify, as well as non-target sequences that should not be amplified.
8p
vikentucky2711
26-11-2020
13
1
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