Sequence characterized amplified regions

Production and consumption of pomegranate have been increasing in the world in recent years due to its health benefits to humans. Sunburn is an important physiological disorder caused by high temperature, light, and radiation. With the changes in climatic conditions that have occurred in recent years, the effect of sunburn is increasing and limiting the production and quality of pomegranate.

14p lyhuyenthu 31-01-2023 10 2   Download

The whole repeat unit of 5S rDNA and the internal transcribed spacer (ITS) of four European Donax species were analysed. After amplifying, cloning and sequencing several 5S and ITS units, their basic features and their variation were described.

13p vihagrid 30-01-2023 11 3   Download

The present study was conducted to characterize Leaf Curl Virus infecting tomato in south Gujarat region showing typical leaf curl symptom with upward/downward curling along with vein clearing of the leaves. A part of DNA-A molecule of ~1200 bp was amplified with a Begomovirus specific primers confirming it to be Begomovirus. On sequencing, a 1125 bp nucleotide sequence (Accession no- KU921251) was obtained. Amplified fragment had two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene.

9p chauchaungayxua6 26-06-2020 11 0   Download

All over the world, Infectious bronchitis (IB) is one of the most fearful diseases of the poultry industry causing significant economic loses. The present study was aimed at the isolation, identification and molecular characterisation of IBV isolates. For the present study, samples were collected from chicken of different age groups that have shown signs of respiratory tract infection. The specific UP and DOWN primers targeting the highly conserved region of 5’UTR were used for the detection of IBV and the positive samples were selected for further characterization.

9p angicungduoc5 12-06-2020 9 1   Download

Marssonina leaf blotch caused by the fungus Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis causing premature leaf fall is one of the major economic constraint which hampers apple production in India. Seven isolates of Marssonina coronaria were isolated and evaluated for their morphological and molecular characterization using ITS primers. All the seven isolates were identified based on the morphological characteristics, and produced brown to black colonies without aerial mycelium, formed a wrinkled surface, on PDA after incubation for 30 days.

8p chauchaungayxua3 07-02-2020 14 0   Download

Vanilla planifolia is popularly known as Prince of Spices a fleshy perennial liana grown in and around Western Ghats for its natural compounds used in several ice creams, chocolates and beverages. Fusarium oxysporum f.sp. vanillae is one of the most destructive pathogens causing severe loss to yield and during the survey conducted in 2016, maximum of 25 % incidence was noticed in coorg district. The pathogen was isolated and morphologically identified as F. oxysporum based on the conidial, chlamydosporial and cultural characters. The size of microconidia ranged between 5.97 to 8.

13p kethamoi2 15-12-2019 10 0   Download

Banana bract mosaic disease (BBrMD) is one of the most important viral diseases of banana which leads to a yield reduction. We have identified banana bract mosaic virus (BBrMV) in banana plants growing in the regions of southern India based on symptomatology, sequence homology, and Serodiagnostic assays. The viral sequence encoding the coat protein was specifically amplified by RT-PCR (Reverse Transcriptase – Polymerase chain reaction) using specific primers bordering the Coat Protein (CP) gene.

12p cothumenhmong1 10-12-2019 16 0   Download

This article examines the development of strain-specific sequence-characterized amplified region (SCAR) molecular markers in two strains of Beauveria bassiana and Metarhizium anisopliae, as well as their use for tracking pathogens in coinfected insect pests.

9p sony2711 28-01-2019 22 1   Download

Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. Th is investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers.

10p vimb123 12-01-2019 28 2   Download