Shoot apical culture

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants. These were free-disease symptoms that were collected for surface sterilization.

8p vijaychest 24-04-2024 3 2   Download

The cultivar Robusta forms the mainstay of commercial banana cultivation in India. An efficient micropropagation system with high multiplication rate will boost banana cultivation and assist banana based industries in the country. The shoot apices of banana cv. Robusta were cultured on two basal media MS and B5 supplemented with different concentrations and combinations of plant growth regulators (PGR). The tissue culture responses were observed for shoot multiplication and effect of subculture on rate of shoot multiplication till 8th subculture.

8p trinhthamhodang5 16-05-2020 16 2   Download

Shoot apices of Physalis minima L. were cultured on fifteen full strength MS medium supplemented with various concentrations of cytokinins (BAP, KIN and TDZ). Tissue culture responses namely elongation of existing shoot and differentiation of multiple shoots were observed from the cultured explants. The establishment of cultured explants was not affected by the cytokinin concentration in the medium but overall shoot apices showed better establishment on medium supplemented with BAP than other cytokinins.

7p nguathienthan4 21-04-2020 20 1   Download

Double sterilized and pre-chilled shoot apices of Galmast cultivar obtained from mature trees (MTSTs) and in vitro raised seedlings (SBSTs) were inoculated on MS medium augmented with BA, Kn and TDZ with and without PG. Increase in BA concentration up to 5.0µM resulted in axillary shoot proliferation in about 40% shoots with a maximum of 14±0.72 shoots per explant. SBSTs (seedling born shoot tips) of same cultivar developed light yellow loose callus (LYLC) when they were cultured on MS(×½) medium fortified with BA(2.0-3.

17p caygaocaolon2 14-03-2020 10 1   Download

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.

9p cothumenhmong1 08-12-2019 18 0   Download

In vitro cultures of five moss species were established on hormone-free MS medium, or on MS medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid and kinetin. In vitro culture of Eurhynchium praelongum (Hedw.) B., S. & G., has been initiated from the apical shoots of the gametophytes, and the cultures of Aloina aloides (K.F.Schultz) Kindb. Brachythecium velutinum (Hedw.) B., S. & G., Ceratodon purpureus (Hedw.) Brid. and Grimmia pulvinata (Hedw.) Sm. were initiated from the spores of immature sporophytes.

6p viminotaur2711 30-10-2019 25 1   Download

Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies.

7p vimb123 11-01-2019 28 2   Download