Shoot tip

In-vitro regeneration of soybean is a prerequisite for successful genetic transformation. This study aimed to investigate the culturability of three soybean cultivars for in-vitro regeneration, focusing on genotypes, media, explant types, and plant growth regulators.

14p viwalton 02-07-2024 1 1   Download

Water availability is a very important factor for the growth and development of plants, which limits the plant production capacity. Rootstocks are widely utilized to improve plants tolerance to various biotic and abiotic stresses. In this study, physiological and biochemical responses of fifteen almond rootstock candidates to drought stress were investigated under in vitro conditions. The shoot tips from fifteen almond rootstock candidates were cultured in MS medium containing 1.0 mg/L BAP, 0.01 mg/L IBA, 30 g/L sucrose and 7 g/L agar.

11p hanthienngao 30-11-2021 14 1   Download

Anther culture is an effective breeding method for improving desirable traits, since it allows fast achievement of genetically homozygous lines. The present studies were carried out to evaluate the response of flue-cured tobacco anthers for high frequency pollen embryo induction in different culture media, and the effects of colchicine treatment on tobacco chromosome doubling. The results showed that different media caused significant changes in pollen embryo induction, in which the most obvious change was found in B medium with induction rates of 23.28%, 26.64%, and 28.

9p tudichquannguyet 29-11-2021 5 1   Download

In this lecture, students will be able to understand: Local Variables, Stack Parameters, Register vs. Stack Parameters, INVOKE Directive, PROC Directive, PROTO Directive, Passing by Value or by Reference, Parameter Classifications, Example: Exchanging Two Integers, Trouble-Shooting Tips.

48p larachdumlanat126 31-12-2020 13 2   Download

The aim of the experiment was to study the effect of media sucrose concentration on shoot proliferation while using shoot tip and axillary bud as explant. The different parameters taken for study were number of shoots per explant, length of shoot (cm) and culture response (%).

5p gaocaolon9 22-12-2020 12 1   Download

The aim of the experiment was to study the effect of media sucrose concentration on shoot proliferation while using shoot tip and axillary bud as explant. The different parameters taken for study were number of shoots per explant, length of shoot (cm) and culture response (%).

5p gaocaolon9 22-12-2020 9 1   Download

The present study was conducted during 2013- 2014 to find out the effect of induced mutation on in vivo growth parameters in „Camarosa‟ strawberry by following CRD factorial design. For the induction of variation explants (Runner tips, shoot tips and leaf disc) were subjected to different concentrations (0.1%, 0.2%, 0.3% and 0.4%) of EMS along with control for various treatment durations (1.5 hr, 2.5 hr and 3.5 hr).

12p angicungduoc8 07-11-2020 14 2   Download

Viewing the above fact the multiplication of papaya through tissue culture is needed for the production of numerous plants within a short period of time that is why the present experiment is proposed.

6p trinhthamhodang1215 23-09-2020 7 1   Download

Henceforth this study is aimed to find out the best combination of growth regulators and optimize its concentration for better regeneration rate of the banana cv. Matti in order to mass produce and popularize the variety to different geographical locations.

11p caygaocaolon7 18-09-2020 13 1   Download

The present investigation was carried out to standardize the plant bio regulators for in vitro shoot proliferation of Curcuma longa L. Cv. Roma. The finger tip of the C. longa was taken as explants and cultured on MS media supplemented with different concentrations of plant bio-regulators.

9p trinhthamhodang7 31-08-2020 15 1   Download

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number.

13p trinhthamhodang7 31-08-2020 9 1   Download

Phyllody is a serious disease of sesame caused by phytoplasma. Different types of disease symptoms are reported to be associated with sesame phyllody. The major symptoms are phyllody, Flower virescence, witches broom, shoot tip fasciation, flattening of the shoot apex and cracking of seed capsules. In addition to this, infected plants exhibit reduction of internode distance and of leaf size. Investigations were carried out on the symptomatology of phytoplasma associated with phyllody disease.

16p angicungduoc5 12-06-2020 12 1   Download

Abscisic acid (ABA) regulates various developmental processes and stress responses over both short (i.e. hours or days) and longer (i.e. months or seasons) time frames. To elucidate the transcriptional regulation of early responses of grapevine (Vitis vinifera) responding to ABA, different organs of grape (berries, shoot tips, leaves, roots and cell cultures) were treated with 10 μM (S)-(+)-ABA for 2 h.

14p vihashirama2711 21-05-2020 14 1   Download

Adventitious root (AR) formation in axillary shoot tip cuttings is a crucial physiological process for ornamental propagation that is utilised in global production chains for young plants. In this process, the nitrogen and carbohydrate metabolisms of a cutting are regulated by its total nitrogen content (Nt), dark exposure during transport and irradiance levels at distinct production sites and phases through a specific plasticity to readjust metabolite pools.

20p vihashirama2711 21-05-2020 16 1   Download

Garlic has lost its ability to form seeds in the course of its domestication. Therefore, the germplasm storage via cryopreservation is increasingly applied. The progression of the various steps within the cryopreservation procedure is accompanied by declining survival rates of the explants.

9p viuchiha2711 21-04-2020 12 0   Download

In the present investigation, a simple, rapid and efficient in vitro regeneration system was developed in green gram (Vigna radiata L. Wilczek) cv. IPM-02-03 using cotyledonary explants excised from a 4 day old seedling. Organogenic calli were developed from cotyledonary tissues within 4-6 weeks of culture, supplemented with 2.5 mg/L BAP and 1.5 mg/L NAA. Shoot regeneration was achieved from shoot tips and cotyledonary node on MS medium was supplemented with BAP 2.0 mg/l

9p cothumenhmong4 23-03-2020 18 2   Download

The experiment was conducted in a polyhouse to study the effect of types of stem cuttings [shoot tip (C1), semi-hardwood (C2) and hardwood cuttings (C3)], growing media [sand (M1), soilrite (M2), soil mixture (M3) and cocopeat (M4)] and plant growth regulator [IBA0 ppm (G0), 500 ppm (G1), 1000 ppm (G2), 1500 ppm (G3) solution and rootex powder (G4)], on rooting and shooting parameters of acid-lime (Citrus aurantifolia (Christm.) Swingle) Cv. Kagzi lime cuttings.

17p caygaocaolon2 14-03-2020 10 0   Download

Double sterilized and pre-chilled shoot apices of Galmast cultivar obtained from mature trees (MTSTs) and in vitro raised seedlings (SBSTs) were inoculated on MS medium augmented with BA, Kn and TDZ with and without PG. Increase in BA concentration up to 5.0µM resulted in axillary shoot proliferation in about 40% shoots with a maximum of 14±0.72 shoots per explant. SBSTs (seedling born shoot tips) of same cultivar developed light yellow loose callus (LYLC) when they were cultured on MS(×½) medium fortified with BA(2.0-3.

17p caygaocaolon2 14-03-2020 9 0   Download

In-vitro propagation is widely used as reliable alternative for true-to-type and quality seed production in sugarcane. The main obstacle under in-vitro propagation is growth of contaminants, resulting in failure of culture establishment that can be minimized by effective surface sterilization method. The sterilization process varied species to species and explants type thus, present study was designed to evaluate the effects of various sterilants on in-vitro culture establishment of sugarcane variety Co 0118 through meristem shoot tip.

8p trinhthamhodang3 12-02-2020 13 1   Download

Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana „Virupakshi‟. Immature male flower bud explant was used for embryogenic callus induction.

8p chauchaungayxua3 07-02-2020 15 2   Download