Single strands dna

Cathelicidins include antimicrobial peptides (AMPs) and are involved in the innate immune defense against infections in mammals. Polymorphisms in the DNA sequence of cathelicidin genes could be relevant to inherited variations of host innate immunity, adaptation, and pathogen resistance. This study aims to investigate the sequence polymorphism of cathelicidin genes including CATHL2 and CATHL4 from local indigenous yellow cattle breeds of Vietnam.

11p dianmotminh02 03-05-2024 11 2   Download

Ðề tài áp dụng kỹ thuật SSCP để phát hiện đột biến trên 3 trình tự của gene pbp2b ở các chủng S. pneumoniae kháng penicillin. Trước hết, chúng tôi xác định một số chỉ tiêu của kỹ thuật để đạt độ phân tích tốt nhất cho các trình tự đã nêu. Các chỉ tiêu này bao gồm: tác nhân biến tính, hàm lượng DNA, nồng độ gel polyacrylamide, phương pháp nhuộm). Sau đó áp dụng quy trình với các chỉ tiêu đã xác định được để phát hiện đột biến trên 20 chủng kháng penicillin so sánh với chủng nhạy. Kết quả cho thấy 18/20 chủng khảo sát có mang đột biến ít nhất trên 1 trong 3 trình tự.

4p nguyentuananh2502 05-04-2019 72 3   Download

For the first time, the detection of DNA sequences, related to HIV (found in AIDS patients), using Methylene Blue (MB) as the redox indicator was performed. The voltametric signals have been investigated on bared chitosan (CS) modified; probe immobilized (DNA single strand) and target-hybridized (DNA double strand) electrodes sequentially by means of Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV). The response of hybridization between the probe and perfectly complementary, double-base mismatch, single-base mismatch of analyzed targets were clearly identified.

5p uocvong03 24-09-2015 38 2   Download

Recently, we have identified and purified minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.

7p system191 01-06-2013 25 4   Download

A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampicillin resistance. Using T4 DNA polymerase and T4 DNA ligase the two primers are physically linked on the template. The non-mutant DNA strand is selected against by growth in the presence of ampicillin.

5p zingzing09 24-04-2013 58 4   Download

DNA ligases are the enzymes responsible for the repair of single-strandedanddouble-strandednicks indsDNA.DNA ligases are structurally similar, possibly sharing a common molecular mechanism of nick recognition and ligation catalysis. This mechanism remains unclear, in part because the structure of ligase in complex with dsDNAhas yet to be solved.DNA ligases share common structural elementswith DNA polymerases, which have been cocrystallized with dsDNA. Based on the observed DNA polymerase–dsDNA interactions, we propose a mechanism for recognition of a single-stranded nick by DNA ligase....

7p tumor12 22-04-2013 39 2   Download

The structural changes produced by the minor-groove binding ligand DAPI (4¢,6-diamidine-2-phenylindole) on individual strands of trinucleotide repeat sequences were detectedby electrophoreticband-shift analysis andrelated to their effects on DNA replication in vitro. Among the 20 possible single-stranded trinucleotide repeats, only theT-rich strand of the AATÆATT triplet exhibits an observable fluorescence band and a change in electrophoretic mobility due to the drug binding.

7p fptmusic 12-04-2013 45 4   Download

There is still a controversy over the mechanism of promoter DNA strand separation upon open transcription complex (RPo) formation by Escheri-chia coli RNA polymerase: is it a single or a stepwise process controlled by Mg 2+ ions and temperature? To resolve this question, the kinetics of pseudo-first-order oxidation of thymine residues by KMnO4 in the )11…+2 DNA region of RPo at thekPRpromoter was examined under single-hit conditions as a function of temperature (13–37
C) in the absence or presence of 10 mmMgCl2....

16p awards 06-04-2013 42 2   Download

TheSCO2299gene fromStreptomyces coelicolorencodes a single peptide consisting of 497 amino acid residues. Its N-terminal region shows high amino acid sequence similarity to RNase HI, whereas its C-terminal region bears similarity to the CobC protein, which is involved in the synthesis of cobalamin. The SCO2299gene suppressed a temperature-sensitive growth defect of anEscherichia coliRNase H-deficient strain, and the recombinant SCO2299 protein cleaved an RNA strand of RNAÆDNA hybridin vitro.

0p awards 06-04-2013 42 1   Download

RecA protein plays a pivotal role in homologous recombination inEscheri-chia coli. RecA polymerizes on single-stranded (ss) DNA forming a nucleo-protein filament. Then double-stranded (ds) DNA is bound and searched for segments homologous to the ssDNA. Finally, homologous strands are exchanged, a new DNA duplex is formed, and ssDNA is displaced. We report a quantitative analysis of RecA interactions with ss d(pN)n of var-ious structures and lengths using these oligonucleotides as inhibitors of RecA filamentation on d(pT)20....

0p awards 06-04-2013 34 3   Download

DNA helicases are molecular
motor enzymes that use the energy of NTP hydrolysis to separate transiently energetic-ally stable duplex DNA into single strands. They are there-fore essential in nearly all DNA metabolic transactions. They act as essential molecular tools for the cellular machinery. Since the discovery of the first DNA helicase in Escherichia coliin1976, several havebeen isolated fromboth prokaryotic and eukaryotic systems.

15p dell39 03-04-2013 50 4   Download

Purified human Rad51 and Rad52 proteins exhibit multiple oligomeric states, in vitro. Single-stranded DNA (ssDNA) renders high molecular weight aggregates of both proteins into smaller and soluble forms that include even the monomers. Consequently, these proteins that have a pro-pensity to interact with each other’s higher order forms by themselves, start interacting with monomeric forms in the presence of ssDNA, presumably reflecting the steps of protein assembly on DNA.

9p dell39 27-03-2013 32 4   Download

Ionizing radiation, oxidative stress and endogenous DNA-damage pro-cessing can result in a variety of single-strand breaks with modified 5¢ and⁄or 3¢ ends. These are thought to be one of the most persistent forms of DNA damage and may threaten cell survival. This study addresses the mechanism involved in recognition and processing of DNA strand breaks containing modified 3¢ ends.

11p dell39 27-03-2013 60 6   Download

T4 DNA ligase is one of the workhorses of molecular biology and used in various biotechnological applications. Here we report that this ligase, unlikeEscherichia coliDNA ligase,TaqDNA ligase and Ampligase, is able to join the ends of single-stranded DNA in the absence of any duplex DNA structure at the ligation site.

10p dell39 27-03-2013 44 3   Download

DnaG is the primase that lays down RNA primers on single-stranded DNA during bacterial DNA replication. The solution structure of the DnaB-helicase-binding C-terminal domain ofEscherichia coliDnaG was determined by NMR spectroscopy at near-neutral pH. The structure is a rare fold that, besides occurring in DnaG C-terminal domains, has been described only for the N-terminal domain of DnaB.

13p inspiron33 23-03-2013 49 4   Download

Helicobacter pylori, an important bacterial pathogen, causes gastric ulcer and gastric adenocarcinoma in humans. The fundamentals of basic biology such as DNA replication are poorly understood in this pathogen. In the present study, we report the cloning and functional characterization of the single-stranded DNA (ssDNA) binding protein from H. pylori.

13p vinaphone15 27-02-2013 31 3   Download

The DMC1 protein, a meiosis-specific DNA recombinase, promotes homol-ogous pairing and strand exchange. The I37N single nucleotide polymor-phism of the human DMC1 protein was reported as a result of human genome sequencing projects.

9p vinaphone15 27-02-2013 26 2   Download

The 3¢-ends of human chromosomal DNA terminate in short single-stranded guanine-rich tandem-repeat sequences. In cancer cells, these are associated with the telomere-maintenance enzyme telomerase together with the end-binding protein hPOT1.

9p mobifone23 18-01-2013 41 5   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks

12p panasonic06 25-12-2011 55 3   Download

Di truyền học Virus I. Đặc tính của các virus 1. Tính đa dạng về cấu trúc và thành phần di truyền Virus có bộ gene rất đa dạng. Bộ máy di truyền của virus có thể là DNA mạch kép (double strand - dsDNA), DNA mạch đơn (single strand ssDNA), RNA mạch kép (dsRNA) hay RNA mạch đơn (ssRNA). Bộ gene RNA của virus là một phân tử hoặc một đoạn, sợi đơn phân cực mạch (+) hoặc mạch (-), có thể ở dạng vòng tròn hay dạng thẳng. Virus nhỏ nhất có nhất có khoảng 4 gene, virus lớn có...

23p poseidon02 16-07-2011 161 41   Download