Skoog medium

This study investigated the effects of different PGRs on in vitro plant regeneration of Anthurium scherzerianum Schott and acclimatization in the ex vitro stage. Depending on the objective of each experiment, the in vitro shoots were induced from callus cultured on a modified Murashige and Skoog (MS) medium containing cytokinins and auxins.

11p vicaptainmarvel 21-04-2023 3 2   Download

An efficient regeneration protocol was established for a rare plant species, Begonia coptidifolia. Using leaf explants, adventitious shoots were induced on Murashige and Skoog (MS) basal medium using various concentrations (1.0–10.0 mM) of three cytokinins, 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (KIN). However, adventitious shoots could not be induced on MS medium supplemented with auxins [α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] tested in the 1.0–10.0 mM range.

8p hanthienngao 30-11-2021 11 1   Download

To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid).

11p hanthienngao 30-11-2021 9 1   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

In this study, we investigated four factors to improve the hairy root induction in Urena lobata L. These factors include: age of plant (15-day-old in vitro plants, 45-day-old in vitro plants and after two subculture generations plants), different parts of plant (roots, stems, and leaves), infection time (10, 20 and 30 minutes), and culture medium (Murashige and Skoog (MS), Gamborg B5 medium (GB5) and Woody plant medium (WPM)).

8p larachdumlanat127 02-01-2021 5 2   Download

Different nutrient solutions alone or in combinations were applied as foliar spray on Asiatic lilium hybrid Tresor and evaluated for different characters. Plants sprayed with only water were taken as control. Other treatments were done with the dose of nutrients used in preparation of tissue culture Murashige and Skoog (MS) medium (1962) in different combinations.

7p caygaocaolon8 07-11-2020 15 2   Download

This study evaluated the effect of particular phytohormones and a plant growth promoting rhizobacteria on the growth and viability of Epithelantha micromeris callus culture. E. micromeris callus growth without the inoculant, diminished in Murashige-Skoog (MS) + naphthalene acetic acid (NAA) medium (34.78%) and MS + indole acetic acid (IAA) (26.08%) experiments.

8p chauchaungayxua8 03-10-2020 15 1   Download

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number.

13p trinhthamhodang7 31-08-2020 9 1   Download

Banana is one of the important fruit crops and food source for million people in developing countries. In the study explants (Suckers) of two banana cultivars were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kinetin sole and in combination for shoot initiation and multiplication.

10p trinhthamhodang5 16-05-2020 10 0   Download

Liquid Murashige and Skoog (MS) medium in varying strengths, is the most commonly utilized medium for proficient rooting in the regenerated plants in doubled haploidy breeding protocols. On the other side, CPV (cocopeat: perlite: vermiculite) medium is currently being used in hardening of tissue culture regenerated plants.

9p angicungduoc4 26-04-2020 15 1   Download

The low cost alternative components of tissue culture media were evaluated for their potential to supply nutrients and to support growing explant of Banana cultivar in vitro for their cost effectiveness against conventional high cost media. The Murashigeand Skoog (MS) salts and sucrose were replaced by vermicompost (50 g/L), table sugar (30 g/L) and coconut water (70 ml/L), and agar was replaced by filter paper, sand and wheat floor. The conventional MS medium supplemented with 30 g/L sucrose and 8 g/L agar was used as control.

7p kethamoi4 18-04-2020 13 1   Download

The present investigation was carried out at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Experiment was laid out using completely randomized design (CRD) with ten replications. Six culture media viz. Murashige and Skoog media, Nitsch & Nitsch media, woody plant medium, Schenk and Hildebrant medium, White’s medium and Khudson solution– C were tested for direct shoot proliferation and callus induction at most responsive level of plant growth regulators in Bael (Aegle marmelos L.).

6p caygaocaolon4 04-04-2020 12 1   Download

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.

7p cothumenhmong3 22-02-2020 14 1   Download

Gladiolus (Gladiolus hybrida) is an important ornamental plant cultivated world over for its attractive spikes. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. In vitro propagation techniques, is superior to conventional propagation and produces disease free plants in huge quantities. In vitro regeneration of gladiolus cultivars, Sylvia, White Prosperity and Amsterdam was achieved using shoot bud of cormels as an explant. Sylvia variety recorded the best for callus induction and regeneration than other two varieties.

6p trinhthamhodang3 14-02-2020 18 0   Download

Arbuscular mycorrhizal (AM) fungi are obligate symbiont and integrate with most of terrestrial plants. AMF belongs to the phylum Glomeromycota that cannot complete their life cycle without establishing a functional symbiosis with host plant. In the present investigation, Glomus intraradices Spores were surface sterilized and transferred to different media viz., one per cent water medium agar medium, Murashige and Skoog medium. Modified Strulla Romand (MSR) medium and white’s medium with root exudates of Agrobacterium rhizogenes MTCC-532 transformed hairy roots of chickpea.

10p nguaconbaynhay3 07-02-2020 8 1   Download

Papaya is one of the few important fruits which flowers and yield throughout the year. Though a polygamous species with three basic types viz., staminate, pistillate and bisexual, its cultivation with seed propagation is hindered by problems due to vide variability in sex expression and fruit characters. In such point of view, propagation through asexual method could be a better alternative. The micropropagation will substitute method of propagation by cutting or grafting would be of great boon to papaya industry. The procedure for in vitro propagation of papaya (Carica papaya) cv.

6p quenchua2 15-12-2019 17 0   Download

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing 1.0 mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, 0.5, 1, 1.5 and 2 mg/l) and five levels of kinetin (0, 0.25, 0.5, 1.0 and 1.5 mg/l) in completely randomized design with 5x5x2 factorial treatment combinations.

9p cothumenhmong1 08-12-2019 18 0   Download

Gerbera (Gerbera jamesonii Bolus) seeds were used as primary source of explants for sterilization. Seeds were sterilized with 0.1 % mercuric chloride for 2 minutes to obtain minimum contamination (10 %). MS (Murashige and Skoog’s media) supplemented with 4 mg per litre BAP + 0.3 mg per litre IAA served as the best medium for shoot proliferation from in vitro shoots as it produced maximum number of shoots (2.67) with maximum number of leaves (4.73) and maximum shoot length (1.92 cm) at three weeks of inoculation.

10p nguaconbaynhay1 04-12-2019 18 1   Download

A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators.

5p viminotaur2711 30-10-2019 14 0   Download

A method for micropropagation was developed for Dendrocalamus asper, an economically and environmentally important bamboo. Disinfected seeds were cultured in flasks containing 20 ml of Murashige and Skoog’s medium (MS) supplemented with BA (1.0-7.0 mg l-1 ) or kinetin (1.0-7.0 mg l-1 ). Multiple shoots (6.53) were formed on MS medium supplemented with 3.0 mg l-1 BA and reached 1.49 cm in length. Continuous shoot proliferation was achieved on a MS medium supplemented with BA (1.0-7.0 mg l -1 ). The multiplication rate of 3.30 fold was achieved on MS medium supplemented with 3.0 mg l-1 BA.

8p trinhthamhodang 24-10-2019 9 1   Download