Sodium dodecyl sulphate
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Aqueous microemulsions (MEs), where an oil coexists with water in the presence of the anionic surfactant sodium dodecyl sulphate (SDS), have been proposed as a solution to decrease the amount of organic solvent in the mobile phase needed in reversed-phase liquid chromatography (RPLC).
10p vironald 15-12-2022 10 2 Download
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The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence.
6p caygaocaolon8 07-11-2020 15 2 Download
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Ten Lactobacillus cultures were considered for the study. Out of ten Lactobacillus cultures, V3 produced maximum β-galactosidase activity (3.381 O.D), followed by NK2 and NK9 up to 24h than other cultures during β-galactosidase activity. Extraction of βgalactosidase was carried out by disruption of Lactobacillus cells using sonication at 3, 4 and 5 minute (pulse 30 second on / 10 seconds off and 35 to 55 % amplitudes), lysozymeEDTA (49 mg of lysozyme in 1.
16p nguathienthan8 20-10-2020 7 1 Download
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Brassica species are major oilseed crops which are an increasingly important part of the human diet worldwide. In present investigation 11 varieties and their 55 F1’s were characterized for storage seed protein profiling using sodium dodecyl sulphate – polyacryl amide gel electrophoresis (SDS - PAGE). The electrophoresis of the proteins revealed protein bands in the range of 80kD to less than 14 kD molecular weight (MW). Out of 15 bands 14 bands were more or less monomorphic and only single band was polymorphic.
8p nguathienthan8 20-10-2020 11 2 Download
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Thirteen (Three gynoecious and ten monoecious) germplasm lines of cucumber (Cucumber sativus L.) were characterized by sodium dodecyl sulphate polyacrylamide vertical slab gel electrophoresis (SDS-PAGE). The seed protein could be resolved into total 11 bands distributed in 4 zones i.e. A, B, C and D. Zone A was divided into 5 subzones and 5 bands, zone B has 1 band C has 5 and zone D included 5 bands. Similarity index value ranged from 62% to 100% among all the genotypes. Pgyn-1 showed least similarity 68% with other genotypes.
9p angicungduoc5 12-06-2020 9 0 Download
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The objective of the research was to study the protein content and electrophoretic profile of specific fractions of Large White Yorkshire (LWY) boar semen. Sperm rich fractions (SRF) of semen were collected from four sexually mature LWY boars as fractions of 10 mL each, by gloved hand method. For analysis, the first 10 mL of the SRF, rest of the SRF and sperm free fraction (SFF) were collected separately.
6p cothumenhmong3 22-02-2020 13 1 Download
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Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa.
8p quenchua2 18-12-2019 8 0 Download
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The present study was undertaken to determine the relationship between MethicillinResistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey.
8p caplock2711 19-02-2019 23 1 Download
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In this study, 18 live specimens collected from six localities in the distribution areas of Mesocricetus brandti and Mesocricetus auratus were examined using SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis). The findings showed that there is no difference in globulin and albumin proteins of M. brandti and M. auratus, and supported the claim that M. auratus is very closely related to M. brandti.
4p caplock2711 19-02-2019 16 0 Download
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The blood serum proteins of 35 live specimens of Rattus rattus and Rattus norvegicus collected from ten localities in Turkey were examined by SDS-PAGE (sodium dodecyl sulphate- polyacrylamide gel electrophoresis).
6p caplock2711 19-02-2019 20 0 Download
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Blood serum proteins of Apodemus flavicollis and Apodemus hermonensis (Mammalia: rodentia) in Turkey
The globulin, albumin, postalbumin, and prealbumin proteins of Apodemus flavicollis and Apodemus hermonensis were examined by SDS-PAGE. In A. flavicollis and A. hermonensis respectively, 6-9 and 7-9 electrophoretic bands were determined in the globulin region, respectively.
4p caplock2711 19-02-2019 18 0 Download
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Globulin and albumin proteins of Apodemus populations in the Black Sea region were examined using SDS-PAGE (sodium dodecyl sulphate - polyacrylamide gel electrophoresis). There were 7-11 bands in the globulin region and 1-3 bands in the prealbumin zone. It was determined that there were variations in both the globulin region and the prealbumin zone in populations of this genus.
7p vitamix 19-02-2019 13 0 Download
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In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization.
7p vitamix 15-02-2019 25 2 Download
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Fifteen species belonging to 3 genera (Onobrychis, Hedysarum and Sartoria), collected from different geographical regions of Turkey were studied for the polypeptide patterns of their seed storage proteins. The variability of seed storage proteins was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).
7p vivant2711 15-02-2019 23 2 Download