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Specific length amplified fragments
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Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with grain yield of wheat (Triticum aestivum L.).
16p
vihagrid
30-01-2023
6
4
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Soybean is not only an important oil crop, but also an important source of edible protein and industrial raw material. Yield-traits and quality-traits are increasingly attracting the attention of breeders. Therefore, fine mapping the QTLs associated with yield-traits and quality-traits of soybean would be helpful for soybean breeders. In the present study, a high-density linkage map was constructed to identify the QTLs for the yield-traits and quality-traits, using specific length amplified fragment sequencing (SLAF-seq).
14p
vitzuyu2711
29-09-2021
22
1
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Drought seriously curtails growth, physiology and productivity in rapeseed (Brassica napus). Although drought tolerance is a complex trait, efficient phenotyping and genotyping has led to the identification of novel marker-trait associations underlying drought tolerance. A diverse panel of 228 Brassica accessions was phenotyped under normal (without stress) and water-stress conditions, simulated by polyethylene glycol (PEG-6000) (15% PEG stress) at the seedling stage; stress tolerance index (STI) and stress susceptibility index (SSI) values were acquired.
15p
covid19
14-06-2020
42
0
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To reveal the genetic loci of the quantitative seed-related traits, we constructed a high-density single nucleotide polymorphism (SNP) linkage map of an F2 population by using specific length amplified fragment (SLAF) technique and determined the quantitative trait loci (QTLs) of seed-related traits for sesame based on the phenotypes of F3 progeny.
20p
viharuno2711
21-04-2020
15
1
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Present study was conducted with the objectives to identify single nucleotide polymorphism in sperm associated antigen 11 B(SPAG11B) gene and to analyze association between identified polymorphism with conception rate in Murrah bulls in ICAR-National Dairy Research Institute (NDRI) herd, Karnal. A 373 base pair region covering partial intron 2, exon 3 and partial intron3 of bovine SPAG11 gene was amplified using genomic DNA extracted from eighty six Murrah bulls and genotyped using sequencing and polymerase chain reaction- restriction fragment length polymorphisms (PCR-RFLP) methods.
7p
cothumenhmong3
22-02-2020
19
0
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Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in pJET1.
9p
chauchaungayxua3
07-02-2020
15
2
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PCR was employed to establish association of begomovirus through amplification of geminivirus specific PCR product. In order to determine the complete nucleotide sequence of DNA-A component of HgYMV, several universal primers/ abutting primers and specific primers available in the literature were tried to amplify full length DNA-A of 2.7 kb. The amplification of full length of DNA-A component of HgYMV was achieved with all the primers such as AC-abut and AV-abut, HgYMVAF and HgYMVAR and HYMVA1500F and HYMV-A1500R.
21p
nguaconbaynhay3
07-02-2020
13
1
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Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. Th is investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers.
10p
vimb123
12-01-2019
28
2
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