Spectrofluorometric method

In this study, a novel spectrofluorometric assay based on p-cresol (4-methyl phenol) probe is developed for the measurement of HOBr scavenging activity. It is the first study involving the use of a p-cresol probe for the determination of the HOBr scavenging activity of biothiols. While the p-cresol probe (λex = 260 nm, λem = 305 nm) has fluorescence characteristics, its brominated derivatives emerging at the end of the oxidation reaction with HOBr do not show fluorescence.

10p tudichquannguyet 29-11-2021 7 0   Download

A simple, rapid, sensitive, and low-cost spectrofluorometric method was developed for the determination of Au(III) using 5-(2,3-dimethoxyphenyl)-3-(4-methoxyphenyl)-1-phenyl-4,5-dihydro-1H-pyrazole (L) in ethanol/water. The method is based on the change in the fluorescence intensity at 464 nm after excitation at 355 nm as a result of the reaction between the ligand and Au(III). The fluorescent emission of ligand at 464 nm decreased with the increasing of Au(III) concentration. The ligand concentration, pH effect, response time, and effect of foreign ions were determined.

15p tudichquannguyet 29-11-2021 3 0   Download