SSR based genetic diversity

The knowledge of the extent and pattern of diversity in the crop species is a prerequisite for any crop improvement as it helps breeders in deciding suitable breeding strategies for their future improvement. Rice is the main staple crop in India with the large number of varieties released every year.

13p vinarcissa 21-03-2023 3 1   Download

To assess the genetic diversity and relationships among 25 cotton (Gossypium hirsutum L.) genotypes were analyzed using morphological and molecular markers. Morphological analysis was performed based on 3 fiber quality traits including fiber length, strength and fineness the mean values of each trait for each genotype were calculated.

12p cothumenhmong11 11-05-2021 8 2   Download

The present investigation studied genetic diversity of ninety four cotton germplasm accessions, in order to improve genetic base of the present cultivars. Out of 48 primer pairs surveyed, 20 (42%) were showing polymorphism. The average number of alleles per locus was 2.45.

8p trinhthamhodang11 27-04-2021 12 1   Download

Hence it can be successfully deployed for selecting desirable genotypes which can be utilized in future breeding programs for exploiting heterosis or in the introgression of genes for biotic and abiotic stress tolerance in soybean crop.

11p trinhthamhodang11 27-04-2021 9 2   Download

The objective of the present study is to reveal the diversity in the inbred lines and testers based on morphological traits and simple sequence repeat markers.

9p chauchaungayxua10 19-03-2021 11 2   Download

An experiment was carried out at AICRP on Potato under Odisha University of Agriculture and Technology, Bhubaneswar to study diversity among morphologically distinguishable potato genotypes with SSR markers. Twenty potato genotypes were laid out in a Randomized Block Design with four replications. Eight morphological characters were observed based on DUS characteristics to visually differentiate genotypes. In addition, fifteen SSR markers were used to depict clear-cut variation among genotypes with the aid of polymorphic bands.

14p trinhthamhodang1215 23-09-2020 16 2   Download

This necessitated a study of heterotic relationships among Iranian maize germplasm. Choukan et al., (2006), using cluster analysis from genetic distance based on SSR makers to evaluate Iranian maize inbred lines reported that the lines could be classified into four preliminary heterotic groups.

13p trinhthamhodang1215 23-09-2020 12 1   Download

This study aimed to evaluate the genetic diversity and to characterize nine pistillate parents of castor (Ricinus communis L.) germplasms by RAPD and SSR markers. The availability of diverse germplasm of any crop is an important genetic resource to mine the genes that may assist in attaining yield as well as quality.

10p caygaocaolon7 18-09-2020 7 1   Download

The reference marker set (30 markers) was used to evaluate the genetic diversity and DNA fingerprinting of 19 standard reference rice varieties. The results showed the similarity coefficient of 19 varieties varied from 0.04 to 0.548. At the genetic similarity coefficient of 0.1, the 19 rice varieties divided into two main groups. Group one included 3 varieties: DH1, DH5, DH13. Group 2 included the remaining 16 varieties. Inside group two, phylogenetic tree divided into two main branches at the genetic similarity coefficient of 0.3.

7p nguyenxuankha_bevandan 14-08-2020 19 2   Download

A set of 45 polymorphic microsatellite markers, representing markers for almost each chromosome, was used for the assessment of genetic diversity in 35 genotypes of bread wheat. A total of 127 alleles were detected which ranged from 2 to 6 with an average allele number of 2.82 per locus. The PIC value of the 45 SSRs ranged from 0.08 to 0.50 with an average PIC of 0.30. Resolving power and Marker index of all 45 markers varied from 0.17 to 2.82 and 0.16 to 1.52, respectively.

11p quenchua6 15-06-2020 7 1   Download

Increased and continued use of the diverse genotypes is a prerequisite for developing and diversifying the genetic base of crop cultivars. DNA markers which are crop-stage nonspecific environmental neutral, easily assayable and amenable for automation are being used to assess the diversity of germplasm accessions and/or breeding lines. DNA markers also provide information on the population structure, allelic richness, and parameters that specify diversity among the genotypes to help breeders to choose those most appropriate for use in cultivar development.

9p kequaidan4 05-05-2020 15 1   Download

Salinity severely limits wheat production in many parts of the world. Development of salt tolerant varieties represents the most practical option for enhancing wheat production from these areas.

15p vishikamaru2711 29-04-2020 13 1   Download

Knowledge of genetic diversity and relationships among breeding materials has a significant impact on crop improvement. Association between parental divergence and progeny performance has not been well documented in cotton (Gossypium hirsutum L.). This study was conducted to estimate genetic diversity based on simple sequence repeat (SSR) markers among the selected elite cotton genotypes belong to different heterotic groups developed at ARS, Dharwad.

9p trinhthamhodang1212 06-04-2020 16 3   Download

The genetic diversity and population structure in 51 chickpea accessions were studied using 30 chickpea specific SSR markers. Twenty eight SSR markers exhibited polymorphism producing a total of 217 alleles. The average number of alleles per locus was 7.75. The average PIC value was 0.75 and ranged from a minimum of 0.53 (TA1) to a maximum of 0.85 (TA64). The markers TA5, TA14, TA18, TA21, TA64, TA71, TA106, TR20, TR26, TR58, TS43 were considered to be highly informative (PIC ≥ 0.8), in evaluating allelic variation present in the chickpea accessions.

13p cothumenhmong4 25-03-2020 11 1   Download

The exploration of genetically variable accessions is the key source of germplasm conservation and potential breeding material for the future. The more diverse group of cultivars provides an ample opportunity to breeders for re-leasing new and superior varieties, considering their quality traits for direct commercial utilization. In this study, we analyzed genetic structure, diversity and relationships in 66 accessions of Cicer arietinum using SSR molecular markers. A total of 44 PCR based SSR primers were used for molecular characterization of 66 accessions.

11p cothumenhmong4 25-03-2020 19 0   Download

In the present study, sixty four aromatic landraces of rice collected from different districts of Madhya Pradesh were analysed for genetic variation using SSR markers. Specially, the objective of the study was DNA fingerprinting and genetic diversity analysis of aromatic landraces to requisite to study the unique feature and to identify the duplication among them and to assist in broadening the germplasm base of future aromatic rice breeding programs. Very high variation was observed for the trait sterile lemma colour in the classes of straw (76.56%), purple (10.94%), gold (7.81%) and 4.

12p nguaconbaynhay3 07-02-2020 17 0   Download

Seed dormancy is an important trait affecting grain yield and quality in cereal crops. It is controlled by its own complicated genetic and environmental factors. The polymorphism pattern study of 24 rice genotypes for RM primers used in the present molecular study were clearly classified the rice genotypes into dormant (Cluster I, Cluster IIA) and nondormant (Cluster II B, Cluster III). In the present study, a total of 40 alleles were detected across the 24 rice genotypes by 11 polymorphic SSR markers. The number of alleles generated per locus by each marker ranged from 2 to 6.

11p nguaconbaynhay3 07-02-2020 11 0   Download

Daffodil (Narcissus spp.) belongs to family Amaryllidaceae and is a bulbous perennial grown for attractive flower, borne in spring sometimes autumn or winter. The present study was carried out to assess the genetic diversity present in daffodils in temperate region of Kashmir India using eleven sample sequence repeats (SSR’s). Twenty seven genotypes of daffodils belonging to different species such as Narcissus incomparabilis, Narcissus pseudonarcissus, Narcissus jonquilla, Narcissus poeticus, Narcissus papyraceus were evaluated for molecular characterization.

10p nguathienthan2 26-12-2019 32 0   Download

Soybean (Glycine max (L.) Merrill] one of nature’s most versatile crops is increasingly becoming an important food and cash crop in the tropics due to its high nutrient quality and adaptability to various growing environments. Soybean is a grain legume crop. As food and feed soybean plays an important role throughout the different countries of the world. It provides oil as well as protein to the living beings. In present study Molecular characterization and genetic diversity assessment of soybean varieties was done using SSR markers.

10p kethamoi2 14-12-2019 34 0   Download

A total of 200 SSR markers from different Brassica species were used in this study. Out of 200 SSR markers analyzed for polymorphism in two parental Brassica juncea genotypes (RB 50, drought tolerant and Kranti, drought susceptible), 51 were polymorphic. The polymorphic markers were used to screen F2 population. A total of 108 alleles were identified in the RB 50 and Kranti and the parental B. juncea genotypes. The PIC (polymorphic information content) values for various primers ranged from 0.340-0.505 with an average of 0.406.

11p nguaconbaynhay1 04-12-2019 14 0   Download