SSRs and Multiplex PCR

The methodology used was quite systematic, rapid, reliable, and appropriate for qualitative detection and can be extended for multiplex development in other plant species having SSR marker technology for efficient plant genetic resources management including genetic diversity analysis, cultivar identification and evolutionary studies in plants.

9p chauchaungayxua10 19-03-2021 10 1   Download

Samba Mahsuri is an elite, fine grain rice variety, which is grown extensively in India. To finger print and identify a genotype specific marker from 26 other closely related fine grain types, we utilized a 113 SSR markers distributed throughout the rice genome. Twenty six markers found to be polymorphic among the rice varieties analyzed. A twin marker combination RM 19426 +JGT 11-16.3 could unambiguously differentiate samba Mahsuri from other fine grain varieties through multiplex PCR.

7p quenchua6 15-06-2020 13 2   Download