Staining methods
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Head and neck cancers, particularly oropharyngeal cancers (OPC), have been increasingly associated with human papillomavirus (HPV) infections, specifically HPV16. The current methods for HPV16 detection primarily rely on p16 staining or PCR techniques.
13p vishanshan 27-06-2024 1 1 Download
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The clinical significance of single cell invasion and large nuclear diameter is not well documented in early-stage oral tongue squamous cell carcinoma (OTSCC). Methods We used hematoxylin and eosin-stained sections to evaluate the presence of single cell invasion and large nuclei in a multicenter cohort of 311 cases treated for early-stage OTSCC.
8p vikoch 27-06-2024 1 1 Download
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The pathological phenotype of perineural invasion (PNI) in squamous cell carcinoma (ESCC) is prevalent but highly heterogeneous. Methods: Postoperative specimens from all patients with ESCC at Shaanxi Provincial People’s Hospital were evaluated for PNI using haematoxylin and eosin (H&E) staining and S100 immunohistochemistry (IHC). We determined the correlation between PNI status and clinical outcomes.
9p vileonardodavinci 23-12-2023 5 2 Download
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The clinical significance of tertiary lymphoid structures (TLSs) is not well-documented in early oral tongue squamous cell carcinoma (OTSCC). Methods: A total of 310 cases of early (cT1-2N0) OTSCC were included in this multicenter study. Assessment of TLSs was conducted on hematoxylin and eosin-stained sections. TLSs were assessed both in the central part of the tumor and at the invasive front area.
6p vileonardodavinci 23-12-2023 8 3 Download
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To investigate the role of NudCD1 in spindle assembly checkpoint regulation and in the prognosis of colorectal cancer. Methods: Immunohistochemical staining was used to detect in situ expression of NudCD1 in 100 colorectal cancer tissue samples. A chi-square test was used to analyse the correlation between the NudCD1 protein expression level of the cancer tissues and clinicopathological features.
13p vialfrednobel 23-12-2023 3 3 Download
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To investigate the expression and prognostic value of c-Jun in hypopharyngeal squamous cell carcinoma (HPSCC). Methods: A retrospective study was performed on a cohort of 99 HPSCC patients. The expression of c-Jun and phosphorylated-c-Jun (p-c-Jun) was evaluated via immunohistochemistry (IHC) staining. Overall survival (OS) and progression-free survival (PFS) were assessed using Kaplan–Meier method and multivariate Cox regression analysis.
11p vialfrednobel 23-12-2023 5 3 Download
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Part 1 book "Hematology techniques and concepts for veterinary technicians" includes content: Introduction to the hematology laboratory, composition of blood, blood volume and effects of blood loss, blood collection and handling, blood smears and staining, routine hematology laboratory tests, automated laboratory methods and instruments.
94p oursky08 04-11-2023 7 5 Download
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Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors with a high prevalence and poor prognosis. It is an urgent problem to deeply understand the molecular mechanism of ESCC and develop effective diagnostic and prognostic methods.
9p vioracle 29-09-2023 4 2 Download
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In this study, anatomical charateristics of this species using double-staining method have been investigated. The anatomical characteristics indicate that S. tonkinensis Doan adapts to a habitat with high moisture and intensive insolation.
5p viberkshire 09-08-2023 4 4 Download
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This paper discussed the influence of outdoor weathering including photo degradation, water/moisture degradation and fungal degradation on the performance of wood in detail. The chemical and physical changes of wood exposed outdoors are described, and methods for protecting exposed wood surfaces are summarized.
10p vilamborghini 12-10-2022 4 4 Download
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This research project will investigate how combining systems of tessellation1 , modularity2 and seriality3 can be utilised with a specific method of paper staining to explore visual pathways within an artwork. A serial system4 of modular tessellation will be implemented, whereby different “sets” of oil-and-pigment soaked paper modules will be constructed and formed into various configurations.
98p runordie3 06-07-2022 8 3 Download
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Food packaging technology: Part 1 has present the content: packaging developments – an historical perspective; food supply and the protective role of packaging; food biodeterioration and methods of preservation; packaged product quality and shelf life; logistical packaging for food marketing systems;...
190p hanthienngao 15-04-2022 31 1 Download
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Epizootic lymphangitis (EL), caused by Histoplasma capsulatum variety farciminosum (HCF) is a contagious, chronic disease of equines, characterized by development of nodular lesions in the lymph nodes, lymphatic vessels and skin. It is one of the most important diseases of equines in Ethiopia, causing significant economic loss, particularly in the livelihood of carthorse owners.
7p vigandhi 23-02-2022 5 1 Download
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There is a growing interest in the scientific community to use computer-based software programs for the quantification of cells during physiological and pathophysiological processes. Drawbacks of computer-based methods currently used to quantify immunohistochemical staining are the complexity of use, expense of software and overly-simplified descriptions of protocol thereby limiting reproducibility.
8p vigandhi 23-02-2022 9 1 Download
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The use of micro-spectroscopy methods is required to study smaller inhalable particles, benefiting from the use of silver membrane filters. Although these filters perform poorly during sample preparation, they can be stained with Nile Red and be used for pre-selection of suspected particles. Future inquiries require method development to better assess smaller size fractions relevant for human exposure.
8p thebadguys 15-01-2022 11 0 Download
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The purpose of this study was to examine whether the administration of nickel (Ni) was associated with cell apoptosis and also to evaluate whether post acute oral exposure to hyperoside would cause histopathologic alterations in rats. Four groups of male SpragueDawley rats (n = 7) used in this study were as follows: a control group and groups treated with Ni (as NiCI2, 13 mg/kg), hyperoside (Hyp) (50 mg/kg), and Ni + Hyp.
10p dolomite36 30-12-2021 6 0 Download
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Mechanism of pro-apoptotic action of prosthetic restorations on oral mThe research was carried out on the saliva of healthy patients using prostheses. The sample swabs were stained using the May-Grünwald-Giemsa method and processed by immunohistochemistry for nuclear factor kappa B (NF-κB; p65) and caspase-3. Western blots were used to detect caspase-3, NF-κB, p53 and COX-2 expression.ucosa cells
7p lazzaro 30-12-2021 10 0 Download
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The FC method using specific nuclear dye DRAQ5 was used to separate BCs from cellular debris. Only 1.82 ± 0.27 % cells were DRAQ5 positive. On the other hand, increase of total events (21 ± 3.30 %) of AnV positive cells without DRAQ5 dye was recorded. Moreover, the affinity of yolk to AnV by FC was also observed (10.45 ± 2.59 %). Significant differences among the groups (P < 0.001) were recorded.
5p mudbound 10-12-2021 13 1 Download
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The present study was done to evaluate the antifungal susceptibility pattern of Candida species by Disc diffusion method which is helpful for guiding therapy. All clinical samples sent to the Microbiology laboratory were inoculated on to Blood agar and MacConkey agar. The suspected colonies of Candida were identified by Gram stain and inoculated onto CHROM Candida differential agar for speciation.
6p trinhthamhodang11 27-04-2021 11 1 Download
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In this study, we investigated CD20+ TILs in triple-negative breast cancer (TNBC) and their relationship with T lymphocyte subsets (CD4+, CD8+, CD25+, and FOXP3+), including their combined prognostic value using an immunohistochemical staining method.
10p vianrose2711 27-04-2021 15 2 Download