Standard biochemical tests

The present study was carried out to detect the sensitivity pattern and prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) at tertiary care hospital, Surendranagar. Total 1776 Clinical specimens were studied. All the isolates were identified by standard microbiological procedures like gram staining, colony morphology on solid culture media and biochemical reactions. Antibiotic sensitivity testing was done for all isolates by modified Kirby Bauer disk diffusion method. Total 194 Staphylococcus aureus were tested for MRSA by Cefoxitin disc diffusion test...

8p cothumenhmong9 18-01-2021 19 1   Download

Urinary tract infection (UTI) is one of the most common infection seen in clinical practice in all age groups. The UTI is most commonly caused by E.coli. There is emergence of multidrug resistant bacteria because of inadvertent use of antibiotics in treating these infections. The changing trend of pathogens and their antibiotic sensitivity the study has been undertaken, to determine the bacteria causing the UTI and find their antibiotic sensitivity. A total of 200 urine specimens showing the significant bacteriuria are studied. The midstream urine samples collected in a sterile container.

5p trinhthamhodang9 16-12-2020 9 1   Download

Blood stream infections can lead to life threatening sepsis and require immediate antimicrobial treatment. Blood culture is an essential tool for the investigation of clinically suspected sepsis. The present study has been conducted to describe the profile of bacterial isolates from blood cultures and their antibiotic resistance. This is prospective study of 273 blood cultures, collected from clinically suspected cases of bacteraemia studied over a period of five months in a tertiary care hospital in Ujjain, M.P.

8p cothumenhmong7 09-09-2020 10 1   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 20 2   Download

The present study was taken up to evaluate the antibiogram against different mastitogenic pathogens isolated from the milk samples of clinical bovine mastitis cases. A total of 78 milk samples were collected from different dairy farmers of Nialiblock, Odisha and processed in Animal Disease Research Institute (ADRI), Phulnakhara, Cuttack for isolation, identification and antibiogram of pathogens. The samples were screened for mastitis by modified California mastitis test (MCMT). The milk samples were inoculated in BHI broth and incubated for 24-48 hours.

6p trinhthamhodang1213 30-05-2020 16 1   Download

To isolate common organisms from suspected cases of septicemia with their antimicrobial susceptibility pattern and know the prevalence of MDR strains by detecting MRSA isolates, ESBL, AmpC and Carbapenemase production. Blood samples were collected from suspected cases of septicemia and incubated in BacT/ALERT 3D. Subculture was done on Blood agar, nutrient Agar, MacConkey Agar and Chocolate Agar and identification was done by standard biochemical reactions.

11p trinhthamhodang1212 06-04-2020 7 0   Download

The study was undertaken to standardize a shelf-stable ready-to-eat pepper chicken using broiler meat by retort pouch processing. Three experiments were conducted, in the first experiment the recipe was standardized (pilot scale study) on the basis of sensory evaluation and in second experiment retort pouch processing temperature (F0 value) was determined based on references, commercial sterility test, visual observation, sensory evaluation and preliminary trials. The come up percentage was 27.27 and the F0 value was 7.2 minutes.

9p quenchua4 06-04-2020 27 4   Download

Salmonella is widely distributed in bovine in different countries and is considered the most important related zoonotic diseases today which have a public health and economic importance. The present study was conducted to survey the prevalence of Salmonellosis in bovine fecal samples collected in ten different herds located at relatively different geographical areas, slaughter house samples and environmental samples by using standard microbiological and biochemical tests and confirm the isolated Salmonella by polymerase chain reaction targeting invA gene, specific at genus level.

9p caygaocaolon2 11-03-2020 15 1   Download

Non-fermenting Gram negative bacilli (NFGNB) are a group of bacteria which are aerobic, non sporing and most commonly they are saprophytes. They are also found as commensals in both man and animals. NFGNB are previously considered as a contaminant but now it is emerged as a important cause for life threatening nosocomial infections and also multidrug resistant organisms. Materials and methods: One year prospective study was conducted and our aim is to characterise the non fermenters from various clinical samples and their antibiogram.

7p caygaocaolon3 09-03-2020 23 0   Download

Blood stream infections are mainly caused by multi drug resistant organisms which more likely prolong the hospital stay and leads to increased health care costs. Hence, early diagnosis and treatment with appropriate antibiotics is the only way to treat bacteraemia. To study the bacteriological profile of the organisms causing septicaemia and their antibiotic resistance patterns to guide the clinicians to initiate the empirical therapy. Blood samples were collected aseptically from patients before taking any antibiotics.

7p cothumenhmong3 22-02-2020 16 0   Download

Chronic Suppurative Otitis Media (CSOM) is a well known disease for its persistence and recurrence in spite of treatment. It was found to be the single major cause of conductive deafness, speech disorders. It involves considerable morbidity and can cause extra and intracranial complications. Due to development of beta lactamase producing microorganisms, drug resistant strains emerge making drug resistance a major cause of treatment failure. The isolation of Methicillin Resistant Staphylococcus aureus (MRSA) was reported within 1year of introduction of methicillin.

9p trinhthamhodang3 22-02-2020 25 0   Download

Blood stream infections (BSIs) can lead to life-threatening sepsis and are globally associated with high morbidity and mortality. Early diagnosis plays a crucial role in managing BSI. Objective is to identify the pathogens causing blood stream infections and to know their antibiotic sensitivity pattern. This was a retrospective study of 1 year duration. A total of 1332 blood samples from clinically diagnosed cases of blood stream infections received in the microbiology laboratory were included in the study.

11p trinhthamhodang3 12-02-2020 30 2   Download

A total of seven microorganisms were isolated from different probiotic sources. The nicotine reducing activity of isolates was tested by general analytical techniques. Amongst them isolates BC6 and BC7 possess maximum nicotine reducing activity therefore it was selected for further investigation. Both isolates could utilize up to 1 g/l nicotine as the sole source of carbon and nitrogen, and optimum nicotine reducing activity obtained at pH 7 by inoculating with inoculum size of 1 McFarland standard units.

8p trinhthamhodang3 12-02-2020 14 2   Download

Coagulase negative staphylococci (CoNS) were generally considered to be contaminants in the past having little clinical significance. Over the past two decades, these organisms have become recognized as important agents of human disease. S. epidermidis is the predominant agent in nosocomial infection, bacteremia, surgical wound and urinary tract infections. Their pathogenic potential is being increasingly understood and also causing problems to clinicians because of their drug resistance.

7p trinhthamhodang3 12-02-2020 10 0   Download

Coagulase negative Staphylococci (CoNS) were generally considered to be contaminants in the past having little clinical significance. Over the past two decades, these organisms have become recognized as important agents of human disease. S. epidermidis is the predominant agent in nosocomial infection, bacteremia, surgical wound and urinary tract infections. Characterization of CoNS isolated from urine samples, their antibiogram and methicillin resistance. The study was conducted from December 2014 to August 2015. We received 518 urine samples, 33 strains of CoNS were isolated.

6p trinhthamhodang3 12-02-2020 20 0   Download

NFGNB also known as Non-fermenters (NFs) are emerging with increasing frequency as agents of opportunistic and often, serious infection as well as nosocomial infection. NFGNB are innately resistant to many antibiotics and are known to produce extended spectrum β-lactamases and metallo β-lactamases (MBL).Very few laboratories in India identify these organisms as routine as they are slow growing and requires special media and biochemical tests for identification. The rates of isolation of NFGNBs are increasing in Clinical Microbiology Laboratory, hence this study was done.

10p nguaconbaynhay3 07-02-2020 24 1   Download

Present study was aimed to characterize Escherichia coli isolated from wound from a hyena. Pus swab collected from wound was processed for bacterial isolation and identification as per standard protocol. Isolate was then subjected to antimicrobial susceptibility test by disc diffusion method using commercial available antibiotics such as ampicillin, amoxycillin, amoxyclav, trimethoprim, chloramphenicol, ciprofloxacin, enrofloxacin, cefepime, cefotaxime, ceftriaxone, ceftriaxone/tazobactum, gentamicin and oxytetracycline.

8p nguaconbaynhay3 07-02-2020 12 0   Download

Bacterial infections are most commonly encountered by clinicians in developing countries. Area-specific monitoring studies done to assess the type of pathogens and their antibiotic sensitivity patterns which help the clinician to opt for best empirical treatment. The hospital based cross sectional study is conducted on various samples collected using sterile techniques from 2474 clinically-suspected cases attending RDBP, Jaipuriya Hospital from Jan 2016 to Dec 2017 and tested bacteriologically using standard conventional procedures.

9p chauchaungayxua3 07-02-2020 24 1   Download

Chronic obstructive pulmonary disease (COPD) is a spectrum of airway diseases that has chronic bronchitis at one end and emphysema at other end. COPD is the major cause of morbidity and mortality worldwide. Most of the episodes (80%) of AECOPD are triggered by infections mainly affecting the lower respiratory tract by respiratory viruses, atypical bacteria and aerobic Gram positive and Gram negative bacteria.

7p quenchua2 15-12-2019 20 1   Download

The study was carried out to investigate bovine tuberculosis and diagnostic potential of IFN-γ assay and necropsy inspection in different abattoirs of Assam and Meghalaya, including its species identification. A total number of 234 animals were screened by IFN-γ and necropsy inspection. IFN-γ was performed according to kit procedures and carcasses were inspected for any gross visible lesion. Species identification was confirmed by biochemical test (viz. Nitrate reduction test, Pyrazinamidase test and Niacin detection test) and PCR targeting pncA and oxyR gene.

9p cothumenhmong1 08-12-2019 14 1   Download