Streamlines

Many biotherapeutic formats leverage antibody light chain affinity chromatography to enable robust manufacturing processes and to streamline process development. These include multi-specific antibody and antibody fragment platforms which are often designed for specific capture purification methods that can provide efficient removal of commonly expressed product-related impurities.

10p vironald 15-12-2022 26 1   Download

The Streamlined Liner of the Pharynx Airway (SLIPA), a type of supraglottic airway, has a non-inflatable cuff that softens at body temperature to fit the laryngeal structure. We investigated whether pre-warming of SLIPA to body temperature may improve insertion parameters.

7p viisaacnewton 25-04-2022 3 1   Download

The third-generation streamlined liner of the pharynx airway (SLIPA-3G) is a new-generation supraglottic airway device (SAD) that is non-cuffed and disposable, with a sealing pressure that varies dynamically with the airway pressure. This study compared the SLIPA-3G with the laryngeal mask airway supreme (LMAS) in patients undergoing laparoscopic cholecystectomy.

6p viisaacnewton 26-04-2022 8 1   Download

Despite the many approaches to study differential splicing from RNA-seq, many challenges remain unsolved, including computing capacity and sequencing depth requirements. Here we present SUPPA2, a new method that addresses these challenges, and enables streamlined analysis across multiple conditions taking into account biological variability.

11p vimichaelfaraday 25-03-2022 14 1   Download

Mycoplasma bovis is an important pathogen of cattle worldwide. Many different clinical manifestations of infection can occur, including respiratory disease, arthritis, and mastitis, causing heavy losses to beef and dairy industries. Because Mycoplasma species are slow-growing and fastidious, traditional identification methods are not cost- or timeeffective, and improved methods are sought to streamline laboratory processes.

6p vidarwin 23-02-2022 7 1   Download

Sequencing technology and assembly algorithms have matured to the point that high-quality de novo assembly is possible for large, repetitive genomes. Current assemblies traverse transposable elements (TEs) and provide an opportunity for comprehensive annotation of TEs. Numerous methods exist for annotation of each class of TEs, but their relative performances have not been systematically compared.

18p vielonmusk 30-01-2022 11 0   Download

Duplex sequencing was originally developed to detect rare nucleotide polymorphisms normally obscured by the noise of high-throughput sequencing. Here we describe a new, streamlined, reference-free approach for the analysis of duplex sequencing data.

10p viaristotle 29-01-2022 7 0   Download

We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium.

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Exploration of large data sets, such as shotgun metagenomic sequence or expression data, by biomedical experts and medical professionals remains as a major bottleneck in the scientific discovery process. Although tools for this purpose exist for 16S ribosomal RNA sequencing analysis, there is a growing but still insufficient number of user-friendly interactive visualization workflows for easy data exploration and figure generation. The development of such platforms for this purpose is necessary to accelerate and streamline microbiome laboratory research.

11p vibeauty 23-10-2021 7 0   Download

Transposome-based technologies have enabled the streamlined production of sequencer-ready DNA libraries; However, current methods are highly sensitive to the amount and quality of input nucleic acid.

16p vitzuyu2711 29-09-2021 7 0   Download

Pontimonas salivibrio strain CL-TW6T (=KCCM 90105 = JCM18206) was characterized as the type strain of a new genus within the Actinobacterial family Microbacteriaceae. It was isolated from a coastal marine environment in which members of Microbactericeae have not been previously characterized.

21p vitzuyu2711 29-09-2021 6 0   Download

Genetically engineered mice (GEM) are essential tools for understanding gene function and disease modeling. Historically, gene targeting was first done in embryonic stem cells (ESCs) derived from the 129 family of inbred strains, leading to a mixed background or congenic mice when crossed with C57BL/6 mice.

20p vigiselle2711 30-08-2021 3 0   Download

Whole-genome sequencing represents a powerful experimental tool for pathogen research. We present methods for the analysis of small eukaryotic genomes, including a streamlined system (called Platypus) for finding single nucleotide and copy number variants as well as recombination events.

14p vikentucky2711 26-11-2020 8 0   Download

MPAgenomics, standing for multi-patient analysis (MPA) of genomic markers, is an R-package devoted to: (i) efficient segmentation and (ii) selection of genomic markers from multi-patient copy number and SNP data profiles. It provides wrappers from commonly used packages to streamline their repeated (sometimes difficult) manipulation, offering an easy-to-use pipeline for beginners in R.

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The nature of somatic mutations observed in human tumors at single gene or genome-wide levels can reveal information on past carcinogenic exposures and mutational processes contributing to tumor development.

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The analysis of viral vector genomic integration sites is an important component in assessing the safety and efficiency of patient treatment using gene therapy. Alongside this clinical application, integration site identification is a key step in the genetic mapping of viral elements in mutagenesis screens that aim to elucidate gene function.

9p viflorida2711 30-10-2020 8 1   Download

Rapid and accurate cancer staging following diagnosis underpins patient management, in particular the identification of distant metastatic disease.

10p vithimphu2711 31-07-2020 9 0   Download

The Vietnamese Government has made remarkable efforts in streamlining its business licensing regulations. Nevertheless, a significant number of constraints remain and their negative impacts on economic reform and development have not been effectively dealt with. This paper discusses the constraints in existing business licensing regulations and provides policy recommendations to improve their quality and enhance the capability of implementing agencies in order to maintain the sustainability of achievements and the quality of any upcoming regulations.

8p nguathienthan6 02-07-2020 18 0   Download

(BQ) This book is designed to introduce you to techniques that you might not know about or even consider because you haven’t been exposed to them before. For example, MATLAB provides a rich plotting environment that not only helps you communicate better, but also makes it possible to present numeric information in a manner that helps others see your perspective. Using scripts and functions will also reduce the work you do even further and this book shows you how to create custom code that you can use to customize the environment to meet your specific needs.

208p nanhankhuoctai5 01-06-2020 6 2   Download

(BQ) The main purpose of MATLAB For Dummies is to reduce the learning curve that is a natural part of using a product that offers as much as MATLAB does. When you first start MATLAB, you might become instantly overwhelmed by everything you see. This book helps you get past that stage and become productive quickly so that you can get back to performing amazing feats of math wizardry.

228p nanhankhuoctai5 01-06-2020 8 0   Download