Stress Analysis Report

This study has been made in an attempt to find the structural integrity of the Chashma Nuclear power plant-1 FA skeleton at room temperature. The finite element (FE) analysis has been performed using ANSYS, in orderto determinethe elongationofthe FAskeleton as wellas the locationofmax. stress and stresses developedin axial direction under tensile load of 9800 N or 2 g being the FA handling or lifting load [Y. Zhang et al., Fuel Assembly Design Report, SNERDI, China, 1994].

6p christabelhuynh 30-05-2020 29 2   Download

Many groups of proteins play important roles in the cell’s response to various stresses. The molecular chaperone GroEL of Escherichia coli represents one such highly conserved family of stress proteins. We have observed that isolated GroEL complexes from stationary cultures contain various polypeptides that can be released from the chaperonin by GroES and/or ATP, and identified two such polypeptides as the proteins GatY and UP12.

9p system191 01-06-2013 31 3   Download

Oviparously developing embryos of the brine shrimp, Artemia franciscana, synthesize abundant quantities of a small heat shock/a-crystallin protein, termed p26. Wild-type p26 functions as a molecular chaperone in vitro and is thought to help encysted Artemia embryos survive severe physiological stress encountered during diapause and anoxia. Full-length and truncated p26 cDNA derivatives were generated by PCR amplification of p26-3-6-3, then cloned in either pET21(+) or pRSETC and expressed in Escherichia coli BL21(DE3)....

10p research12 01-06-2013 58 5   Download

Here we show, for the ®rst time, thein vitro formation of ®lamentous aggregates of phosphorylated tau protein in SH-SY5Y human neuroblastoma cells. The formation of suchaberrant aggregates, similar to thoseoccurringinvivoin Alzheimer's disease and other tauopathies, requires okadaic acid, a phosphatase inhibitor, to increase the level of phos-phorylated tau, and hydroxynonenal, a product of oxidative stress that selectively adducts and modi®es phosphorylated tau.

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Mycobacterium tuberculosisheat shock protein 16.3 (MTB HSP 16.3) accumulates as the dominant protein in the latent stationary phase of tuberculosis infection. MTB HSP 16.3 displays several characteristics of small heat shock proteins (sHsps): its expression is increased in response to stress, it protects against protein aggregationin vitro, and it contains thecoreÔa-crystallinÕdomainfound inall sHsps.

8p research12 23-04-2013 36 2   Download

We have characterized the biochemical properties of the testis and brain-specific 105-kDa protein which is cross-reacted with an anti-bovine HSP90 antibody. The protein was induced ingermcells byheat stress, resulting inaprotein which is one of the heat shock proteins [Kumagai, J., Fuk-uda, J., Kodama, H., Murata, M., Kawamura, K., Itoh, H. &Tanaka,T. (2000)Eur. J.Biochem.267, 3073–3078]. In the present study,we characterized thebiochemical propertiesof the protein.

10p tumor12 22-04-2013 30 3   Download

Four distinct eukaryotic initiation factor 2a(eIF2a)kinases phosphorylate eIF2aat S51and regulateprotein synthesis in response to various environmental stresses. These are the hemin-regulated inhibitor (HRI), the interferon-inducible dsRNA-dependent kinase (PKR), the endoplasmic reticu-lum (ER)-resident kinase (PERK) and the GCN2 protein kinase. Whereas HRI and PKR appear to be restricted to mammalian cells, GCN2 and PERK seem to be widely distributed in eukaryotes.

14p tumor12 20-04-2013 29 4   Download

Glucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mam-mals. In fish, the expression and regulation ofGLUT in relation to hypoxia remains unexplored. Here we describe the identification ofa hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans
11 kb ofgenomic sequence and consists of12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly-peptide of533 amino acids, with a predictedmolecularmass of
57 kDa and a pI of8.34....

8p fptmusic 16-04-2013 42 3   Download

a-Crystallin, a molecular chaperone and lens structural protein protects soluble enzymes against heat-induced aggregation and inactivation by a variety of molecules. In this study we investigated the chaperone function ofa-crys-tallininamorephysiological systeminwhicha-crystallinwas incorporated into red cell
ghosts. Its ability to protect the intrinsic membrane protein Na/K-ATPase from external stresses was studied. Red cell ghosts were created by lysing the red cells and removing cytoplasmic contents by size-exclusion chromatography. ...

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The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigasto hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridiza-tion method was used to identify specific hypoxia up- and downregulated genes, in gills, mantle and digestive gland, after 7–10 days and 24 days of exposure....

18p fptmusic 12-04-2013 49 3   Download

The small heat shock proteins function as molecular chaperones, an activ-ity often requiring reversible oligomerization and which protects against irreversible protein denaturation. An abundantly produced small heat shock protein termed p26 is thought to contribute to the remarkable stress resistance exhibited by encysted embryos of the crustacean,Artemia francis-cana. Three novel sequence motifs termed G, R and TS were individually deleted from p26 by site-directed mutagenesis.

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Fromin vivo models of stroke it is known that ischemia⁄reperfusion indu-ces oxidative stress that is accompanied by deterioration of brain mito-chondria. Previously, we reported that the increase in Ca 2+ induces functional breakdown and morphological disintegration in brain mitochon-dria subjected to hypoxia⁄reoxygenation (H⁄R). Protection by ADP indica-ted the involvement of the mitochondrial permeability transition pore in the mechanism of membrane permeabilization.

9p fptmusic 11-04-2013 26 2   Download

Eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). In this case, so-called unfolded protein response (UPR) genes are induced. We determined the transcriptional expression ofArabidopsis thaliana UPRgenes by fluid microarray analysis of tunicamycin-treated plantlets. Two hundred and fifteen up-regulated genes and 17 down-regulated ones were identified.

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The protective role of reactive oxygen scavengers against photodamage was studied in isolated photosystem (PS) I submembrane fractions illumin-ated (2000lEÆm )2 Æs )1 ) for various periods at 4
C. The photochemical activity of the submembrane fractions measured as P700 photooxidation was significantly protected in the presence of histidine orn-propyl gallate. Chlorophyll photobleaching resulting in a decrease of absorbance and fluorescence, and a blue-shift of both absorbance and fluorescence maxi-mum in the red region, was also greatly delayed in the presence of these scavengers. ...

0p awards 05-04-2013 60 4   Download

This paper reports the results obtained using the osmotic stress method applied to the purified cathodic and anodic hemoglobins (Hbs) from the catfishHoplosternum littorale, a species that displays facultative accessorial air oxygen-ation. We demonstrate that water potential affects the oxy-gen affinity ofH. littoraleHbs in the presence of an inert solute (sucrose). Oxygen affinity increases when water activity increases, indicating that water molecules stabilize thehigh-affinitystateof theHb.

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Jasmonic acid and its methyl ester are ubiquitous plant sig-nalling compounds necessary for the regulation of growth and development, as well as for the response of plants to environmental stress factors. To date, it is not clear whether methyl jasmonate itself acts as a signal or if its conversion to jasmonic acid is mandatory prior to the induction of a def-ense response. We have cloned a cDNA, encoding a methyl jasmonate-cleaving enzyme, from tomato cell suspension cultures.

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Bcp2 was identified as a putative peroxiredoxin (Prx) in the genome data-base of the aerobic hyperthermophilic archaeonSulfolobus solfataricus.Its role in oxidative stress was investigated by transcriptional analysis of RNA isolated from cultures that had been stressed with various oxidant agents.

11p dell39 27-03-2013 35 4   Download

Neurodegenerative disorders are associated with oxidative stress. Low den-sity lipoprotein (LDL) exists in the brain and is especially sensitive to oxi-dative damage. Oxidative modification of LDL has been implicated in the pathogenesis of neurodegenerative diseases. Therefore, protecting LDL from oxidation may be essential in the brain.

10p inspiron33 26-03-2013 44 4   Download

Oviparous development in the extremophile crustacean,Artemia franciscana, generates encysted embryos which enter a profound state of dormancy, termed diapause. Encystment is marked by the synthesis of p26, a polydis-perse small heat shock protein thought to protect embryos from stress.

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Oxidant-induced cell damage may be initiated by peroxidative injury to lysosomal membranes, catalyzed by intralysosomal low mass iron that appears to comprise a major part of cellular redox-active iron. Resulting relocation of lytic enzymes and low mass iron would result in secondary harm to various cellular constituents.

12p inspiron33 25-03-2013 40 3   Download