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Studies on molecular characterization
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In medical genetics, discovery and characterization of disease trait contributory genes and alleles depends on genetic reasoning, study design, and patient ascertainment; we suggest a segmental haploid genetics approach to enhance gene discovery and molecular diagnostics.
24p
viellison
28-03-2024
3
2
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Ebook "Molecular response functions for the polarizable continuum model: Physical basis and quantum mechanical formalism" presents the main aspects of the response functions theory (RFT) for molecular solutes described within the framework of the Polarizable Continuum Model (PCM). PCM is a solvation model for a Quantum Mechanical molecular system in which the solvent is represented as a continuum distribution of matter.
70p
coduathanh1122
27-03-2024
3
0
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This study aimed to characterize the methicillin-resistant S. aureus strains circulating on the skin of individuals with acne. S. aureus isolates were screened for methicillin resistance by methods including, oxacillin agar (OA), cefoxitin disk diffusion (CDD), and PCR for the mecA gene.
9p
viplato
02-01-2024
4
2
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Part 2 book "Advances in veterinary dermatology (Vol 8)" includes content: Bacterial resistance to antimicrobial agents and its impact on veterinary and human medicine, molecular and epidemiological characterization of canine Pseudomonas otitis using a prospective case-control study design, staphylococcus aureus penetrate the interkeratinocyte spaces created by skin-infiltrating neutrophils in a mouse model of impetigo,... and other contents.
148p
oursky10
07-12-2023
4
3
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Bladder cancer (BLCA) represents a highly heterogeneous disease characterized by distinct histological, molecular, and clinical features, whose tumorigenesis and progression require aberrant metabolic reprogramming of tumor cells. However, current studies have not expounded systematically and comprehensively on the metabolic heterogeneity of BLCA.
18p
vioracle
29-09-2023
9
2
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Alphonso is the most delicious variety of mango (Mangifera indica L.) known for its excellent texture, taste, and richness with vitamins and minerals. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on morphological characteristics, quality, yield and molecular assessment of mango. A plot of 16 acres lands used for this study with already grown mango trees.
6p
viisac
23-09-2023
2
0
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The study "Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30" was set out to characterize African swine fever virus genotypes from 20 farms in 10 provinces, which occurred ASF outbreaks in 2019 and 2020. The results are expected to contribute sequence data for molecular epidemiology studies and provide further understanding of ASF disease patterns in Southern Vietnam.
8p
tieuvulinhhoa
22-09-2022
7
2
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This study aims to detect and characterize DCH among domestic cats in Malaysia. A cross-sectional study was performed on 253 cats, of which 87 had paired blood and liver samples, entailing whole-genome sequencing and phylogenetic analysis of DCH from a liver tissue sample.
10p
vidarwin
23-02-2022
16
1
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Molecular and cellular changes are intrinsic to aging and age-related diseases. Prior cross-sectional studies have investigated the combined effects of age and genetics on gene expression and alternative splicing; however, there has been no long-term, longitudinal characterization of these molecular changes, especially in older age.
16p
vielonmusk
30-01-2022
11
0
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The new heterobimetallic [Mg(II)-Ti(IV)]-µ-oxoisopropoxide was synthesized on treatment of magnesium acetate with titanium(IV) isopropoxide in refluxing decalin in 1:2 molar ratio and characterized by liberated isopropanol, elemental, spectral (IR, 1H and 13C NMR, and mass), thermal analysis, and molecular weight data.
9p
langthannam
29-12-2021
7
0
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All the complexes showed nonelectrolytic behavior. Moreover, the newly synthesized mixed ligand complexes were evaluated for their in vitro antimicrobial efficiency against bacteria and yeast. The compound named Co(L1 L) had good antifungal activity against Candida species, but no profound antibacterial effect against bacterial strains.
13p
langthannam
29-12-2021
8
0
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Thioredoxin reductase (E.C 1.6.4.5.; TrxR) is an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. In this study, mitochondrial TrxR enzyme was purified from rainbow trout mitochondria. Thanks to the 2 consecutive procedures (preparation of homogenate and 2’,5’-ADP Sepharose 4B affinity chromatography), the enzyme, having the specific activity of 11.9 EU mg protein-1, was purified with a yield of 2.38% and 672-fold. The purity of the enzyme was monitored and the molecular weight of its subunits was calculated as 70 kDa by SDS-PAGE.
10p
langthannam
29-12-2021
9
0
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The thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with 3.07 EU × mg −1 specific activity was purified 990-fold in a yield of 2.05% using 2’,5’-ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be 45.5 kDa by SDSPAGE. The molecular mass of the enzyme’s natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography.
13p
langthannam
29-12-2021
10
0
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Heterocyclic compounds with diaryl substituents have been a milestone approach for selective cyclooxygenase 2 (COX 2) inhibition by bioisosteric replacements and modifications. It is also known that thiazole derivatives have different pharmacological activities. In this study, nine novel 2-[(1,5-diphenyl-1H-imidazole-2-yl)thio]-N-(thiazole-2-yl)acetamide derivatives (Compound 1-9) were synthesized via the reaction of 1,5-disubstitued phenyl-imidazole-2-thiole and N-thiazole acetamide. The inhibitory effects of COX-1 and COX-2 enzymes were tested for the synthesized compounds
45p
langthannam
29-12-2021
12
0
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The effects of polyethylene glycol (PEG), PEG concentration, molecular weight, sodium citrate, and sodium chloride (NaCl) on yield and purification factor were studied. The highest purification factor (4.5) and yield (83%) were obtained in system PEG 6000 (14% w/w)-sodium citrate (18% w/w) with 6% (w/w) NaCl.
6p
mudbound
10-12-2021
11
0
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Potato dry rot disease (Fusarium spp.) is one of the most important fungal storage rots on potato tubers after harvesting. The aims of this study were to identify the Fusarium species that cause dry rot in potato storages, evaluate the most virulent Fusarium species in vitro and in vivo for fungicide sensitivity and determine the susceptibility of the cultivars. In this context, as a result of surveys carried out in potato storages of Ödemiş and Torbalı counties in İzmir province in 2015–2016; Fusarium species obtained from diseased potato tubers were identified as Fusarium sambucinum, F.
12p
hanthienngao
30-11-2021
10
2
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Atriplex mollis Desf. (Amaranthaceae), a North African endemic halophytic species, is further described in this study. Phylogenetic analysis based on a combined dataset of ITS and ETS rDNA and atpB-rbcL and trnK cpDNA showed that A. mollis is closely related to the Malta- and Gozo-endemic Cremnophyton lanfrancoi Brullo & Pavone. Given this close phylogenetic relationship, A. mollis is also considered among the oldest species of Atriplex, together with C. lanfrancoi. Molecular data also suggest that A. mollis in North Africa, C. lanfrancoi on Malta Island, and Atriplex cana Ledeb.
12p
tudichquannguyet
29-11-2021
8
2
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The results showed that the polymer additive with the alkyl chain C14H29- demonstrated the best flow improvement performance. At a concentration of 1000 ppm, this polymer additive reduced the pour point of waste cooking oil-based biodiesel from 12 to 5o C. In this study, the effect of alkyl chain length, molecular weight, as well as the concentration of the additives on the pour point of biodiesel was also discussed.
6p
princessmononoke
29-11-2021
11
1
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Orobanche cumana is a parasitic plant that can lead to advanced losses in yield of agricultural lands cultivating sunflower, depending on the sunflower varieties and the level of contamination. In our study, genetic diversity of six O. cumana populations from the Thrace region of Turkey was determined with the help of eight SSR (microsatellite) loci. All SSR loci were polymorphic. A total of 23 alleles were determined for the analyzed 120 samples. Allele number of each SSR locus ranged from two to six. Mean number of alleles (Na = 2.271), effective allele number (Ne = 1.
10p
tudichquannguyet
29-11-2021
8
1
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In this research, a series of La0.7Sr0.3Co1−yFeyO3−δ (y = 0.0–0.4) was successfully synthesized by a solidstate reaction method and syngas production studies were performed using gas chromatography. Thermogravimetric analysis characterized the concentration of Fe3+, which gave a low H2/CO ratio over La0.7Sr0.3Co1−yFeyO3−δ without the presence of molecular oxygen. All samples possessed a purely rhombohedral structure. The incorporation of more Fe increased the lattice parameter and changed XRD peaks of the La1−xSrxCo0.8Fe0.2O3 from sharp and single to double.
9p
tudichquannguyet
29-11-2021
7
0
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