Sugarcane somaclones

DNA was extracted from plants and RAPD analysis was performed for fourteen treatments with two random oligonucleotide primers (OPA-01 and OPB-11). Among fourteen treatments, plants derived from the treatments T9, T11, and T12 produced polymorphism T12 was found to exhibit maximum polymorphism at 1.0 µM EMS in 2009T5. The clone, 2009T5 produced more variation than 2008T42. Sodium nitrite did not produce any variation.

8p chauchaungayxua5 08-05-2020 15 1   Download

An experiment was carried out at the Agricultural Research Station, Perumallapalle for the regeneration of somaclones. Two sugarcane clones, 2008T42 (susceptible to red rot) and 2009T5 (susceptible to smut) were used as plant material. Calli from the controls and different concentrations of mutagenic chemicals (sodium nitrite and EMS) were transferred on to MS media with 3 mg l-1 BAP, 2 mg l-1 IAA and 2 mg l-1 kinetin for evaluating the effect on shooting.

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Present investigation was carried out to screen two promising sugarcane varieties CoN 13073 and CoN 09072 for salinity tolerance through callus culture. Various physiological and morphological parameters was studied, maximum shoot length was observed at 2.0 % NaCl concentration treated to callus at 65 days of culturing in variety CoN 13073, similar trend was observed for root length and leaf number per plant in variety CoN 13073 at 2.0 % NaCl concentration..

9p caygaocaolon2 14-03-2020 11 1   Download