Supplemented with BA

In this study, the effect of plant growth regulators (BA and NAA), ripe bananas in combination with potato, humic acid, and silver nanoparticles (AgNPs) were investigated to find a suitable condition for in vitro plant regeneration from R. imschootiana protocorm-like body (PLB). The results showed that after 45 days of culture, MS medium supplemented with 100 g ripe banana/liter and 100 g potato/liter was suitable for regenerating shoots from the protocorm-like body (PLB) (17.56 shoots/PLBs and 100.00% of shoot-regenerating PLBs).

13p dianmotminh02 03-05-2024 4 1   Download

Nghiên cứu "Phân lập và tuyển chọn các chủng vi khuẩn nội sinh có khả năng cố định đạm, phân giải lân, tổng hợp IAA từ cây lúa" tiến hành phân tích 30 chủng vi khuẩn đã được phân lập trên môi trường RMR (Rennie medium supplemented with rice extract and malate) từ thân, lá, rễ của các giống lúa trồng tại Gia Lâm – Hà Nội. Kết quả cho thấy, ba chủng N6, N15, N26 có các khả năng cố định đạm, phân giải lân và tổng hợp IAA tốt nhất. Mời bạn tham khảo chi tiết.

8p ngoccthanh 30-06-2022 24 3   Download

Hanoi city has abundant groundwater, supplemented by the surface water (mainly Red river) all year round, and the extensive shallow aquifers, which are easily exploited by large-diameter wells. There always exists some open hydrogeological windows in the Red river area with an open structure; therefore, the groundwater has a strained hydraulic relationship with the Red river water system Along the Red river from Ba Vi to the end of Phu Xuyen district, there are nine regions with three types and four sub-types of different hydrogeological structures.

12p vielonmusk 21-01-2022 19 0   Download

An efficient regeneration protocol was established for a rare plant species, Begonia coptidifolia. Using leaf explants, adventitious shoots were induced on Murashige and Skoog (MS) basal medium using various concentrations (1.0–10.0 mM) of three cytokinins, 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (KIN). However, adventitious shoots could not be induced on MS medium supplemented with auxins [α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] tested in the 1.0–10.0 mM range.

8p hanthienngao 30-11-2021 11 1   Download

Roses are one of the most popular fl owers in the world because of their beauty, color, aroma as well as their various uses and Red Eden rose is one of the most popular hybrid roses. Results of the study showed the most optimal medium for shoot multiplication, which was MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 0.5 mg/l of BA. The optimal medium for growth and fl owering was ½MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 30 μM of AgNO3 . However, NAA at all tested concentrations did not show any eff ect on in vitro plant growth.

7p nguaconbaynhay12 03-06-2021 23 2   Download

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number.

13p trinhthamhodang7 31-08-2020 9 1   Download

The aim of this study was to isolate and optimize the growth conditions of thermophilic microorganism from hot springs. The isolation was conducted by using the mineral salt basal medium supplemented with 0.6% yeast extract at 500C. Totally, 33 isolates of thermophilic microorganism were isolated from hot springs at Truong Xuan (Khanh Hoa province) and Binh Chau (Ba Ria - Vung Tau province).

6p tomhiddleston 27-05-2020 9 1   Download

Double sterilized and pre-chilled shoot apices of Galmast cultivar obtained from mature trees (MTSTs) and in vitro raised seedlings (SBSTs) were inoculated on MS medium augmented with BA, Kn and TDZ with and without PG. Increase in BA concentration up to 5.0µM resulted in axillary shoot proliferation in about 40% shoots with a maximum of 14±0.72 shoots per explant. SBSTs (seedling born shoot tips) of same cultivar developed light yellow loose callus (LYLC) when they were cultured on MS(×½) medium fortified with BA(2.0-3.

17p caygaocaolon2 14-03-2020 9 0   Download

Violar tricolor belongs to Viola genus, Violaceae family, Violar tricolor plant is not only grown as an ornamental plant, but also a medicinal herb. In this study, some of factors affect on the process of micropropagation were investigated. In the experiment of sterilization explants, seed germination and shoot formation ability of the Violar tricolor L. reached the best result when they were sterilized by 0.1% HgCl2 for 1 minute.

8p bangchungthep4 09-03-2020 32 1   Download

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.

7p cothumenhmong3 22-02-2020 14 1   Download

The aim of this study was to isolate and optimize the growth conditions of thermophilic microorganism from hot springs. The isolation was conducted by using the mineral salt basal medium supplemented with 0.6% yeast extract at 500C. Totally, 33 isolates of thermophilic microorganism were isolated from hot springs at Truong Xuan (Khanh Hoa province) and Binh Chau (Ba Ria - Vung Tau province). The effects of temperature (45 - 800C), pH (pH 6 - 9) and carbon sources (malate, pyruvate, acetate, glucose, fructose, or carbon dioxide) on the growth of isolates were examined.

6p sabiendo 02-01-2020 18 0   Download

A study on the development of in vitro propagation protocol for newly developed mutants of Carnation (Dianthus caryophyllus L.)” was carried out during 2017-2018. Results reveal that all the mutants responded well to the in vitro propagation, however ‘UHFS Car-4’ exhibited best propagation potential. Calcium Hypochlorite (5%) treatment for 20 min. is suggested for surface sterilization of explants as it gives 100% uncontaminated growing cultures. For initial culture establishment, MS medium supplemented with 2.0 mgl-1 BA proved to be most effective resulting in earliest establishment.

13p kethamoi2 14-12-2019 12 1   Download

The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, 0.5 g/l AC, 7 g/l agar, 0.1 mg/l NAA and 1 mg/l BA were optimal for PLB formation (8.67 PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, 0.5 g/l AC, and 7 g/l agar after about 90 days.

8p slimzslimz 08-12-2019 12 1   Download

A method for micropropagation was developed for Dendrocalamus asper, an economically and environmentally important bamboo. Disinfected seeds were cultured in flasks containing 20 ml of Murashige and Skoog’s medium (MS) supplemented with BA (1.0-7.0 mg l-1 ) or kinetin (1.0-7.0 mg l-1 ). Multiple shoots (6.53) were formed on MS medium supplemented with 3.0 mg l-1 BA and reached 1.49 cm in length. Continuous shoot proliferation was achieved on a MS medium supplemented with BA (1.0-7.0 mg l -1 ). The multiplication rate of 3.30 fold was achieved on MS medium supplemented with 3.0 mg l-1 BA.

8p trinhthamhodang 24-10-2019 9 1   Download

This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA.

9p viartemis2711 22-10-2019 13 1   Download

Effects of culture media and genotypes of water chestnut (Trapa sp.) on the frequency of callus induction from anther culture were investigated. Results revealed that the N6 media supplemented with 0.5 mg/l BA, 0.5 mg/l NAA and 0.1 mg/l GA3 were suitable for callus induction.

5p vitamix 15-02-2019 12 1   Download

An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (0.5, 1.0, or 2.0 mg/L) alone or in combination with 0.5 mg/L α-naphthalene acetic acid (NAA).

7p vimb123 11-01-2019 15 2   Download

We report here the protocol for Zedoary (Curcuma zedoaria Roscoe) callus and cell suspension cultures. The MS medium supplemented with 2% sucrose, 1.0 mg/l 2,4-D and 1.0 mg/l BA was effective for callus induction from in vitro leaf-base explants of Zedoary. During subcultures, secondary proliferated calli were subsequently produced from initial induced calli on the MS medium with 0.5 mg/l 2.4-D and 0.5 mg/l BA. These calli were light yellow in color, compact and friable.

7p tuanlocmuido 13-12-2012 69 4   Download