Synechocystis

Two ORFs, cphA and cphB, encoding proteins CphA and CphB with strong similarities to plant phytochromes and to the cyanobacterial phytochrome Cph1 of Synechocystis sp. PCC 6803 have been identified in the filamentous cyanobacterium Calothrix sp. PCC7601. While CphA carries a cysteine within a highly conserved amino-acid sequence motif, to which the chromophore phytochromobilin is covalently bound in plant phytochromes, in CphB this position is changed into a leucine.

10p system191 01-06-2013 39 4   Download

This studywas designed to yield data on the supramolecular organization of the phycobilisome apparatus from Synechocystis, and the possible effects of environmental stress on this arrangement. Phycobilisomes were dissociated ina low ionic strength solutionandaquantitative estimation of the protein components present in each subcomplex was obtained using liquid chromatography coupled on-line with a mass spectrometer equipped with an electrospray ion source (ESI-MS).

9p research12 29-04-2013 50 3   Download

Chlorophyll biosynthesis requires a metabolic dialog between the chloroplast envelope and thylakoids where biosynthetic activities are localized. Here, we report the ®rst plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase (MgPIXMT) sequence identi®ed in the Arabidopsisgenome owing to its similarity with the Synechocystis sp.

9p research12 29-04-2013 39 3   Download

The PsbH protein, a small subunit of the photosystem II complex (PSII),was identi®edas a6-kDaproteinband in the PSII core and subcore (CP47±D1±D2±cytb-559) from the wild-type strain of the cyanobacteriumSynechocystisPCC 6803. The protein was missing in the D1±D2±cyto-chromeb-559 complex and also in all PSII complexes isolated from IC7, a mutant lacking the psbHgene.

10p research12 23-04-2013 26 3   Download

Genes homologous to hydrogenase accessory genes are scattered over the whole genome in the cyanobacteriumSynechocystissp. PCC 6803. Deletion and insertion mutants of hypA1 (slr1675), hypB1 (sll1432), hypC, hypD, hypE and hypF were constructed and showed no hydrogenase activity. Involvement of the respective genes in maturation of the enzyme was con-firmed by complementation. Deletion of the additional homologueshypA2 (sll1078) and hypB2(sll1079) had no effect on hydrogenase activity. Thus, hypA1andhypB1are specific for hydrogenase maturation....

12p tumor12 20-04-2013 31 4   Download

Two isoforms of a heme oxygenase gene,ho1andho2, with 51% identity in amino acid sequence have been identified in the cyanobacterium Synechocystissp. PCC 6803. Isoform-1, Syn HO-1, has been characterized, while isoform-2, Syn HO-2, has not. In this study, a full-lengthho2gene was cloned using synthetic DNA and Syn HO-2 was demonstrated to be highly expressed inEscherichia colias a soluble, catalytically active protein.

0p awards 05-04-2013 38 2   Download

Heme oxygenase (HO) catalyzes the oxidative degradation of heme utilizing molecular oxygen and reducing equiva-lents. In photosynthetic organisms, HO functions in the biosynthesis of such open-chain tetrapyrroles as phyto-chromobilin and phycobilins, which are involved in the sig-nal transduction for light responses and light harvesting for photosynthesis, respectively.

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Sulfoquinovosyl diacylglycerol (SQDG) is involved in the maintenance of photosystem II (PSII) activity in Chlamydomonas reinhardtii[Minoda, A., Sato, N., Nozaki, H., Okada, K., Takahashi, H., Sonoike, K. & Tsuzuki, M. et al.(2002) Eur. J. Biochem.269, 2353–2358]. To under-stand the spread of the taxa in which PSII interacts with SQDG, especially in cyanobacteria, we produced a mutant defective in the putativesqdBgene responsible for SQDG synthesis from two cyanobacteria, Synechocystis sp.

9p dell39 03-04-2013 45 3   Download

The His332 residue of the D1 protein has been identified as the likely ligand of the catalytic Mn ions in the water oxi-dizing complex (Ferreira, K.N., Iverson, T.M., Maghlaoui, K., Barber, J. & Iwata, S. (2004) Science303, 1831–1838). However, its function has not been fully clarified. Here we used thermoluminescence and flash-induced chlorophyll fluorescence measurements to characterize the effect of theD1-H333E,D1-H332DandD1-H332Smutationson the electron transport of Photosystem II in intact cells of the cyanobacteriumSynechocystis6803....

10p dell39 03-04-2013 71 5   Download

DNA-binding sites for SYCRP1, which is a regulatory protein of the cyanobacterium Synechocystissp. PCC6803, were predicted for the whole genome sequence by estimating changes in the binding free energy (DDG A total ) for SYCRP1 for those sites. The DDG A total values were calculated by summingDDGvalues derived from systematic single base-pair substitu-tion experiments (symmetrical and cooperative binding model).

10p inspiron33 23-03-2013 39 4   Download

Soluble inorganic pyrophosphatases (inorganic diphosphatases, EC 3.6.1.1) were isolated and characterized from three phylogenetically diverse cyano-bacteria)Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Pseudanabaenasp. PCC 6903 – and one anoxygenic photosynthetic bacter-ium, Rhodopseudomonas viridis(purple nonsulfur).

12p galaxyss3 21-03-2013 43 5   Download

In order to identify an enzyme capable of Fenton reaction inSynechocystis, we purified an enzyme catalyzing one-electron reduction oft-butyl hydro-peroxide in the presence of FAD and Fe(III)-EDTA. The enzyme was a 26 kDa protein, and its N-terminal amino acid sequencing revealed it to be DrgA protein previously reported as quinone reductase [Matsuo M, Endo T and Asada K (1998)Plant Cell Physiol39,751–755].

10p galaxyss3 21-03-2013 39 2   Download

The cyanobacterial plasma membrane is an essential cell barrier with func-tions such as the control of taxis, nutrient uptake and secretion. These functions are carried out by integral membrane proteins, which are difficult to identify using standard proteomic methods.

14p galaxyss3 21-03-2013 37 3   Download

Synechocystissp. PCC 6803 exhibits light-activated heterotrophic growth (LAHG) under dark conditions with glucose as a carbon source. The light activation is remarkable at a late period of photoautotrophic preculture, such as the late-linear and stationary growth phases.

12p vinaphone15 27-02-2013 37 4   Download

Biliverdin-IXareductase fromSynechocystisPCC6803 (sBVR-A) is a stable dimer and this behaviour is observed under a range of conditions. This is in contrast to all other forms of BVR-A, which have been reported to behave as monomers, and places sBVR-A in the dihydrodiol dehydrogenase⁄N-ter-minally truncated glucose–fructose oxidoreductase structural family of dimers.

12p vinaphone15 25-02-2013 49 3   Download

The charged quaternary ammonium compounds – methyl, ethyl and benzyl viologens – generate reactive oxygen species in photosynthetic cells. Three independent methyl viologen-resistant spontaneous mutants of Synechocys-tis sp. PCC 6803 were identified, in which the conserved R115 residue of the Slr1174 protein was replaced with G115, L115 and C115. The Slr1174 protein of the DUF990 family is related to the permease subunit of an ABC-2-type transporter and its R115 mutation was found to be solely responsible for the observed methyl viologen resistance. ...

11p vinaphone15 25-02-2013 30 2   Download

Retinoids are C20 apocarotenoids that have various important functions in metazoans. In addition, several findings suggest their occurrence in eubac-teria, including cyanobacteria. It has been shown that the Synechocystis cytochrome P450 enzyme CYP120A1 is a retinoic acid-binding polypeptide. In this work, we determined the reaction catalyzed by CYP120A1 and investigated its substrate specificity in vitro.

16p viettel02 20-02-2013 31 3   Download

Cyanobacterial NAD(P) + -reducing reversible hydrogenases comprise five subunits. Four of them (HoxF, HoxU, HoxY, and HoxH) are also found in the well-described related enzyme from Ralstonia eutropha.

9p cosis54 09-12-2012 30 1   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta

12p panasonic06 26-12-2011 63 5   Download