Synthetic media

This study aimed to establish culture medium changing system to improve the efficiency of bovine somatic cell nuclear transfer protocol. Our results showed that using the culture medium of Takahashi and First 1992 produced the cloned bovine blastocyst at the highest rate (9.4%, P < 0.05) among different modified synthetic oviduct fluid (mSOF) media.

4p visergey 02-04-2024 4 0   Download

Ebook "Advances in food mycology (Advances in experimental medicine and biology, Volume 571)" was attended by some 35 participants, drawn from among food mycology and related disciplines around the world. The workshop was highly successful, with papers devoted to media and methods development in food mycology, as is usual with these workshops. Particular emphasis was placed on the fungi which produce mycotoxins, especially their ecology, and through ecology, potential control measures.

375p manmanthanhla0201 27-02-2024 7 2   Download

Ebook "Hierarchical bottom-up methodology for integrating dynamic ethynylhelicene oligomers: Synthesis, double-helix formation, and the higher-assembly formation" describes an unprecedented bottom-up approach using synthetic ethynylhelicene oligomers, which form dynamic double helices in organic media. Oligomer synthesis, homo- and hetero-double helix formation, and higher assembly formation due to intercomplex interactions are documented.

98p nhanphanguyet 28-01-2024 2 2   Download

The repertoire of complex proteins produced by the host cell during monoclonal antibody (mAb) production has generated a bottleneck in downstream bioprocessing. Low ppm levels of host cell proteins (HCPs) must be achieved atthe downstream purification process stage to generate an end product suitable for use in humans.

11p viginny 23-12-2022 4 3   Download

Aschersonia aleyrodis (Webber) one of the fungal entomopathogens utilized as a bio-control agent and was reported as the cause of epizootics amongst citrus black fly populations. The present study was aimed to find out cost effective suitable medium for mass multiplication of Aschersonia aleyrodis. Influence of synthetic, semi-synthetic culture media and food grains as solid substrate on growth and sporulation of A. aleyrodis was studied under laboratory condition.

9p caygaocaolon6 30-07-2020 13 1   Download

The aim of the present study was to optimize a process for production enzyme Laccase via microbial fermentation using peels of Ash Gourd. It is rich in various nutritional substances which are required for culture of microorganisms.. By estimating proximate values of various substances a synthetic media was prepared for Trichoderma Viride. Laccases were collected extra and intracellular level. The enzyme activity, growth was observed which were treated in various conditions and purified via various steps i.e. Ammonium Sulphate and dialysis.

7p nguaconbaynhay6 24-06-2020 10 0   Download

The effect of different media (Oat meal agar, Corn meal agar, Czapeck’s Dox agar, Potato dextrose agar, Richard’s synthetic agar and 2% malt extract agar) on light wavelengths (red, yellow, blue, green) and temperatures (10, 15, 20, 25, 30, 35⁰ C) were examined to determine optimal conditions for mycelial growth of Curvularia lunata causing Curvularia leaf spot of maize under in vitro conditions. Amongst media under different temperatures, maximum mycelial growth (88.17 mm) was found in Potato dextrose agar 30⁰ C followed (84.33 mm) by Potato dextrose agar at 25⁰ C.

4p trinhthamhodang1213 02-06-2020 18 1   Download

Different agar media, including natural, Oat Meal Agar (OMA), Malt Extract Agar (MEA), V8 Agar and Leaf extract agar of host plant, semi-synthetic media, Potato Dextrose Agar (PDA), carrot dextrose agar (CaDA) and synthetic media, Czapex Dox Agar (CDA), Richards Synthetic Agar (RSA), Asthana and Hawkers Agar (AHA)) were used to observed the mycelial growth rate, cultural and morphological characters of four fungal isolates after seven days of incubation at 26±1°C.

8p caygaocaolon5 27-05-2020 13 1   Download

Trichoderma are free-living fungi and found commonly in soil - root ecosystem. They are highly interactive in root, soil and foliar environments. They produce or release a variety of compound that induces localized or systemic resistance responses in plant. In this investigation ten different synthetic media (TSM, PDA, Asthana & Hawkers, Richard’s agar, Sabouraud’s dextrose, Rose Bengal agar, Czapex Dox, Beef extract agar, Cooke rose Bengal and Pikovaskys agar) used to study the morphological and cultural characters of the bio-control agent.

6p angicungduoc4 26-04-2020 11 0   Download

Cultural and morphological variability studies on three different media viz., Potato Dextrose Agar, Richard’s Synthetic Agar and Czapek’s Dox Agar revealed considerable variation among the isolates of A. burnsii indicated the existence of variability in the pathogen. Moreover, Potato Dextrose Agar and Czapek’s Dox Agar were found as an excellent media to support the growth and spore formation of isolates of A. burnsii, respectively.

14p cothumenhmong3 22-02-2020 18 0   Download

The cole crops like cabbage, cauliflower, broccoli and brussel sprouts etc. are most important vegetables consumed all over the world, among them cabbage and cauliflower are economically vegetables in India. Most of the cruciferous vegetables are vulnerable to many insect pests. The Diamondback moth (DBM), Plutella xylostella Lineaus is the most serious pest in causing economic loss. DBM developed resistant to almost all the synthetic insecticides. Here we collected DBM population from Field of Rattihalli, Haveri district of the state Karnataka, India and reared for one generation.

9p kequaidan2 13-12-2019 10 1   Download

Chilli is grown in many states in India as a valuable trade crop. Chilli accounts for 20-30% of total Indian spices exports valuing approx Rs. 400-500 cores. Nine cultural media Potato dextrose agar, Host extract agar (Chilli), Oat meal agar, Carrot agar, Plane agar, Richards agar, Czapeks agar, Elliots agar, Saboroud’s agar were analyzed for in vitro mycelial growth of Sclerotium rolfsii. The cultural characteristics indicated that the excellent growth and sclerotia formation were observed on different semi-synthetic media viz.

6p cothumenhmong1 11-12-2019 12 1   Download

A series of 1-(1,3,4-thiadiazolo)-2-thioxo-pyrimidines 4a-g are synthesized by the condensation of monosubsituted thioureas la-g, ethyl acetoacetate 2 and aryl aldehyde 3 in dry media using microwave irradiation. The reactions are completed in very short time with high yield. The use of inorganic solid supports has been proved as an alternative mean of synthetic media.

6p angicungduoc1 02-12-2019 13 0   Download

The exclusive use of plant juices, not as a mere supplement to synthetic culture media, for culturing rhizospheric microorganisms (RMO) is introduced here. Juices were prepared from desert (Mesembryanthemum crystallinum L., Zygophyllum album L., Carpobrotus edulis L.) as well as cultivated (Trifolium alexandrinum L., Beta vulgaris L.) plants. Colonies of RMO (Azospirillum brasilense, Enterobacter agglomerans and Klebsiella pneumoniae) nicely developed on surfaceinoculated agar plates prepared from crude and diluted juice of M. crystallinum (ice plant).

9p kethamoi1 17-11-2019 8 0   Download

Our previous publications and the data presented here provide evidences on the ability of plantbased culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested chemically-synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homogenates, crude saps, juices and powders of cactus (Opuntia ficus-indica) and succulent plants (Aloe vera and Aloe arborescens) were rich enough to support growth of rhizobacteria. Representative isolates of Enterobacter spp.

12p kequaidan1 16-11-2019 21 1   Download

The plant-based-sea water culture medium is introduced to in vitro cultivation and in situ recovery of the microbiome of halophytes. The ice plant (Mesembryanthemum crystallinum) was used, in the form of juice and/or dehydrated plant powder packed in teabags, to supplement the natural sea water. The resulting culture medium enjoys the combinations of plant materials as rich source of nutrients and sea water exercising the required salt stress. As such without any supplements, the culture medium was sufficient and efficient to support very good in vitro growth of halotolerant bacteria.

14p caygaocaolon1 13-11-2019 18 0   Download

Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development.

5p sony2711 01-02-2019 17 1   Download

We investigate the transient absorption phenomena in synthetic diamonds obtained by High Pressure High Temperature (HPHT) and Chemical Vapor Deposition (CVD) methods. The aim of our study is to search inorganic crystalline media combining ionizing radiation and investigate their detecting properties and non-linear absorption of ultra-short laser pulses. The pump-probe technique with 140 fs laser pulses was used for the investigations.

7p thuyliebe 08-10-2018 18 0   Download

Vi khuẩn không sản sinh Indol, men của nó không làm đông sữa, làm đỏ sữa qùy, không sản sinh khí và acid từ đường saccharose (Ou, 1972). Nguồn cacbon tốt nhất là đường glucose, galactose, suctose và nguồn đạm tốt nhất là glutamid acid, aspartic acid, methionine, cystine và asparagine. Môi trường nuôi cấy thường dùng là Wakimoto’s potato semi-synthetic media, nhiệt độ tối thích là 26–30oC và pH là 6–6,5.

18p bachtuocpaul 16-04-2013 137 22   Download

Plastics have had a significant influence on industrial, domestic and cultural aspects of everyday life in the twentieth and twenty-first centuries. They represent advances in technology, illustrated by the dramatic growth in number and type of information storage media available since the 1970s, credit and payment cards, medical applications and food containers which can be taken directly from freezer to microwave oven to dinner table. The development of plastics reflects economic history.

301p coeus75 29-01-2013 73 9   Download