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T7 expression system
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Cloning and expression of recombinant VP2 capsid protein gene of canine parvovirus in E. coli System
In the present study, new CPV-2a field strain (KLD3) isolated in cell culture was selected and the whole CPV VP2 gene was used for the expression in the E. coli expression system. Prokaryote expressing monocistronic DNA cassette containing open reading frame of whole CPV capsid gene (VP2) downstream of T7 promoter was synthesized. Analysis of the expression in E. coli cells showed the presence of capsid protein. Recombinant capsid protein showed immunoreactivity similar to the whole CPV virus antigen, when reacted with polyclonal antibodies against the whole CPV virus particles.
10p
angicungduoc5
12-06-2020
5
1
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Isopropyl-β-D-1-thiolgalactopyranoside (IPTG)-inducible expression of recombinant proteins in E. coli is commonly used and effective. Nevertheless, unintended induction was encountered as a problem when using these bacterial expression systems, generating cultures that give reduced or variable protein yields.
10p
viuchiha2711
21-04-2020
10
2
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The metallo-b-lactamase (MBL) GOB-1 was expressed via a T7 expression system in Escherichia coliBL21(DE3). The MBL was purified to homoge-neity and shown to exhibit a broad substrate profile, hydrolyzing all the tested b-lactam compounds efficiently.
12p
cosis54
05-01-2013
39
2
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