TAG synthesis
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Part 1 of book "Speech and Language Processing: An introduction to natural language processing" provide with knowledge about: regular expressions and automata; words and transducers; N-grams; word classes and part-of-speech tagging; hidden markov and maximum entropy models; phonetics; speech synthesis; automatic speech recognition; speech recognition: advanced topics;...
509p britaikridanik 06-07-2022 35 3 Download
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RNA levels detected at steady state are the consequence of multiple dynamic processes within the cell. In addition to synthesis and decay, transcripts undergo processing. Metabolic tagging with a nucleotide analog is one way of determining the relative contributions of synthesis, decay and conversion processes globally.
17p viaristotle 29-01-2022 11 0 Download
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The optimized nanophosphors were used to visualize latent fingerprints through recognizing level I-III ridges by a reduced background hindrance and can be utilized for visualizing latent fingerprints on difficult substrate surfaces with superior sensitivity, high contrast and selectivity. Further, a furthermost possible stochastic process to make unclonable anti-counterfeiting tags using optimized nanophosphor was designed to reduce counterfeit products.
10p larachdumlanat129 14-01-2021 10 2 Download
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Given the importance of relation or event extraction from biomedical research publications to support knowledge capture and synthesis, and the strong dependency of approaches to this information extraction task on syntactic information, it is valuable to understand which approaches to syntactic processing of biomedical text have the highest performance.
13p vicoachella2711 27-10-2020 9 1 Download
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Oil from seeds of the tung tree (Vernicia fordii) has unique drying properties that are industrially important. We found that the extended oil accumulation period was related to the high seed oil content at maturity among tung tree population.
17p vishikamaru2711 29-04-2020 8 1 Download
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Triacylglycerols (TAGs) are the main composition of plant seed oil. Long-chain acyl-coenzyme A synthetases (LACSs) catalyze the synthesis of long-chain acyl-coenzyme A, which is one of the primary substrates for TAG synthesis.
14p viharuno2711 21-04-2020 9 1 Download
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Antimicrobial peptides have become important candidates as new antibiotics against resistant bacterial strains. However, the major industrial manufacture of antimicrobial peptides is chemical synthesis with high costs and in relatively small scale.
10p viuchiha2711 21-04-2020 6 0 Download
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Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially available eukaryotic in vitro translation systems by its flexibility, high translation efficiency and success rate of properly folded eukaryotic protein synthesis.
9p vihamax2711 21-04-2020 22 2 Download
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VanXYC, a bifunctional enzyme from VanC-phenotype Enterococcus gallinarum BM4174 that catalyses D,D-peptidase and D,D-carboxypeptidase activities, was purified as the native protein, as a maltose-binding protein fusion and with an N-terminal tag containing six histidine residues. The kinetic parameters of His6–VanXYC were measured for a variety of precursors of peptidoglycan synthesis involved in resistance: for D-Ala-D-Ala, the Km was 3.6 mM and kcat, 2.5 s)1; for UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-DAla (UDP-MurNAc-pentapeptide[Ala]), Km was 18.8 mM and kcat 6.
7p system191 01-06-2013 36 2 Download
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The initial condensation event in the nonribosomal biosyn-thesisof thepeptideantibioticsgramicidinSandtyrocidineA takes place between a phenylalanine activating racemase GrsA/TycAand the ®rst proline-activatingmodule ofGrsB/ TycB. Recently we established a minimalin vitro model system for NRPS with recombinant His6-tagged GrsA (GrsA Phe-ATE; 127 kDa) and TycB1 (TycB1Pro-CAT; 120 kDa) and demonstrated the catalytic function of the C-domaininTycB1Pro-CAT to form a peptide bond between phenylalanine and proline during diketopiperazine formation (DKP). ...
10p research12 23-04-2013 31 1 Download
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Stringent factor is a ribosome-dependent ATP:GTP pyrophosphoryl trans-ferase that synthesizes (p)ppGpp upon nutrient deprivation. It is activated by unacylated tRNA in the ribosomal amino-acyl site (A-site) but it is unclear how activation occurs. A His-tagged stringent factor was isolated by affinity-chromatography and precipitation. This procedure yielded a protein of high purity that displayed (a) a low endogenous pyrophosphoryl transferase activity that was inhibited by the antibiotic tetracycline; ...
0p awards 05-04-2013 38 1 Download
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GenesSNO1 and SNZ1 are Saccharomyces cerevisiae homologues ofPDX2andPDX1which participate in pyri-doxine synthesis in the fungusCercospora nicotianae.In order to clarify their function, the two genesSNO1and SNZ1were expressed inEscherichia colieither individually or simultaneously and with or without a His-tag. When expressed simultaneously, the two protein products formed a complex and showed glutaminase activity.
8p dell39 03-04-2013 39 2 Download