Tandem technology
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Technological limitations have hindered the large-scale genetic investigation of tandem repeats in disease. We show that long-read sequencing with a single Oxford Nanopore Technologies PromethION flow cell per individual achieves 30× human genome coverage and enables accurate assessment of tandem repeats including the 10,000-bp Alzheimer’s disease-associated ABCA7 VNTR.
16p vielonmusk 30-01-2022 5 0 Download
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Tandem repeat (TR) expansion is the underlying cause of over 40 neurological disorders. Long-read sequencing offers an exciting avenue over conventional technologies for detecting TR expansions. Here, we present Straglr, a robust software tool for both targeted genotyping and novel expansion detection from long-read alignments.
20p viarchimedes 26-01-2022 6 0 Download
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Simple sequence repeats (SSRs) are tandem repeats of DNA that have been used to develop robust genetic markers. These molecular markers are powerful tools for basic and applied studies such as molecular breeding. In the model plants in Nicotiana genus e.g. N. benthamiana, a comprehensive assessment of SSR content has become possible now because several Nicotiana genomes have been sequenced. We conducted a genomewide SSR characterization and marker development across seven Nicotiana genomes.
12p vibeauty 23-10-2021 8 1 Download
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Here we improved the reconstruction of HCMV full genomes by means of a hybrid, de novo genome-assembly bioinformatics pipeline upon data generated from the recently released MinION MkI B sequencer from Oxford Nanopore Technologies.
12p vitzuyu2711 29-09-2021 17 1 Download
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In this paper, the RNA sequencing and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) technologies were used to study the transcriptome and metabolite profiles of the roots of continuously cropped soybeans that were infected with F. mosseae and F. oxysporum.
13p vijichea2711 28-05-2021 8 1 Download
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The productivity of sugar beet is strongly limited by several biotic stresses, among them rhizomania is one of the important factor causing yield loss of 20–50% or more. Beet necrotic yellow vein virus is the etiological agent of the destructive disease.
10p cothumenhmong9 18-01-2021 12 1 Download
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This article focuses on personal environment for learning a language deeply. The study aims to raise awareness of ways in which digital Personal Learning Environments (PLE) can be used in tandem with more formal learning strategies for blended learning.
8p trinhthamhodang8 02-11-2020 11 1 Download
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Five diseases are currently screened on dried blood spots in France through the national newborn screening programme. Tandem mass spectrometry (MS/MS) is a technology that is increasingly used to screen newborns for an increasing number of hereditary metabolic diseases.
10p virubber2711 21-03-2020 12 1 Download
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There are always a wide range of opinions about internet and social media and how useful or harmful, it can be. As a marketer, I can say for certain that used in the right way, it can be a very powerful communication tool and an unsurpassed platform for engagement. There’s a lot to learn about internet and social media, and a huge variety of social media that you could employ, but this article will give you a strong start on knowing what’s out there and how it can help you.
6p nguaconbaynhay3 07-02-2020 19 0 Download
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Phage display of antibody libraries is a powerful tool for antibody discov-ery and evolution. Recombinant antibodies have been displayed on phage particles as scFvs or Fabs, and more recently as bivalent F(ab¢)2.We recently developed a technology (E-clonal) for screening of combinatorial IgG libraries using bacterial periplasmic display and selection by fluores-cence-activated cell sorting (FACS) [Mazor Yet al.
13p mobifone23 21-01-2013 46 3 Download
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This first chapter is an introduction to PHP, MySQL, and the interaction of the two. In it, we’ll try to address some of the most common questions about these tools, such as “What are they?” and “How do they compare to similar technologies?” Most of the chapter is taken up with an enumeration of the many, many reasons to choose PHP, MySQL, or the two in tandem.
20p vantrungtran 03-02-2010 124 18 Download