Technology and medicine

An applied field of biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. (http://en.wikipedia.org/wiki/Biotechnology) It is the products and exploitation from other fundamental sciences.Traditional Biotechnology Traditional biotechnology refers to a number of ancient ways of using living organisms to make new products or modify existing ones. In its broadest definition, traditional biotechnology can be traced back to human's transition from hunter-gatherer to farmer.

29p zingzing09 24-04-2013 64 9   Download

As science and technology has developed, the manner by which drugs can be delivered has grown. Medicines may be taken orally, applied as topical pastes, creams or oils, patches or administered by direct injection to the body. This research explores an alternative method for the delivery of therapeutic compounds to the body. The basis of the study involves the application of traditional medicinal compounds used to cure minor neck and back pains through transdermal topical application, using a textile substrate as the carrier.

128p runthenight07 01-03-2023 13 3   Download

The goad of the thesis is to build the manufacture process of nano-sized magnetic fluids based on iron oxide (uniform particle size and high magnetic saturation) with stable technology; Characteristic research of magnetic properties of magnetic nanoparticles; assessment of toxicity and test of effects on cells, aiming to make contrast medicine in imaging diagnosis by magnetic resonance imaging (MRI), application on accurately identifying cancer.

28p xacxuoc4321 08-07-2019 45 5   Download

Objective of topic To conduct research on some biological characters of strains of King Oyster and Yunzhi mushrooms to identify best developing strains in climatic condition of Vietnam, and at the same time, to set up spawn production technologies for mushroom strains of liquid spawn in order to improve production efficiency for edible and medicinal mushrooms

27p fujijudo87 07-07-2014 98 10   Download

Human glandular kallikrein 2 (hK2) is a trypsin-like serine protease expressed predominantly in the prostate epithelium. Recently, hK2 has proven to be a useful marker that can be used in combination with prostate specific antigen for screening and diagnosis of prostate cancer. The cleavage by hK2 of certain substrates in the proteolytic cascade suggest that the kallikrein may be involved in prostate cancer development; however, there has been very little other progress toward its biochemical characterization or elucidation of its true physiological role.

8p system191 01-06-2013 50 2   Download

Affinity reagents capable of selective recognition of the different human immunoglobulin isotypes are important detection and purification tools in biotechnology. Here we describe the development and characterization of affinity proteins (affibodies) showing selective binding to human IgA. From protein libraries constructed by combinatorial mutagenesis of a 58-amino-acid, three-helix bundle domain derived from the IgG-binding staphylococcal protein A, variants showing IgA binding were selected by using phage display technology and IgA monoclonal antibodies (myeloma) as target molecules. ...

9p system191 01-06-2013 39 3   Download

The Department of Biochemistry and Molecular Biology, 2CREST of Japan Science and Technology Corporation; 3The Department of Nephrology and Endocrinology, Faculty of Medicine, The University of Tokyo, Japan We have cloned cDNA for leukotriene B4 12-hydroxydehydrogenase (LTB 4 12-HD)/15-ketoprostaglandin 13-reductase (PGR) from guinea-pig liver. LTB4 12-HD catalyzes the conversion of LTB4 into 12-keto-LTB4 in the presence of NADP1, and plays an important role in inactivating LTB4.

9p system191 01-06-2013 42 4   Download

Institute for Organic Chemistry, University of Karlsruhe, Germany; 2Institute for Organic Chemistry, Budapest University of Technology and Economics, Hungary Elucidation of the 3D structure of histidine ammonia-lyase (HAL, EC 4.3.1.3) from Pseudomonas putida by X-ray crystallography revealed that the electrophilic prosthetic group at the active site is 3,5-dihydro-5-methylidene-4H-i´ midazol-4-one (MIO) [Schwede, T.F., Retey, J., Schulz, G.E. (1999) Biochemistry, 38, 5355 –5361].

9p system191 01-06-2013 50 3   Download

Insulin-like growth factor (IGF) binding to the type 1 IGF receptor (IGF1R) elicits mitogenic e€ects, promotion of di€erentiation and protection from apoptosis. This study has systematically measured IGF1R binding anities of IGF-I, IGF-II and 14 IGF analogues to a recombinant high-anity form of the IGF1R using BIAcore technology. The analogues assessed could be divided into two groups: (a) those designed to investigate binding of IGF-binding protein, which exhibited IGF1R-binding anities similar to those of IGF-I or IGF-II;...

8p research12 01-06-2013 41 4   Download

CD40 is areceptorwithnumerous functions in theactivation of antigenpresentingcells (APCs), particularlydendritic cells (DC). Using phage display technology, we identified linear peptides containing a novel FPGN/S consensus sequence that enhances the binding of phage to a purified murine CD40-immunoglobulin (Ig) fusion protein (CD40-Ig), but not to Ig alone. To examine the ability theFPGN/Speptides to enhance adenoviral infection of CD40-positive cells, we used bifunctional peptides consisting of an FPGN-contain-ing peptide covalently linked to an adenoviral knob-binding peptide (KBP)....

8p fptmusic 16-04-2013 33 4   Download

Antisense agents are valuable tools to inhibit the expression of a target gene in a sequence-specific manner,and may be used for functional genomics,target validation and thera-peuticpurposes.Three typesof anti-mRNAstrategies canbe distinguished. Firstly,the use of single stranded antisense-oligonucleotides; secondly,the triggering of RNA cleavage through catalytically active oligonucleotides referred to as ribozymes; and thirdly,RNA interference induced by small interfering RNA molecules.

17p fptmusic 16-04-2013 41 2   Download

Numerous innovations in high-throughput protein production and micro-array surface technologies have enabled the development of addressable formats for proteins ordered at high spatial density. Protein array imple-mentations have largely focused on antibody arrays for high-throughput protein profiling. However, it is also possible to construct arrays of full-length, functional proteins from a library of expression clones.

12p fptmusic 12-04-2013 45 4   Download

While cardiac hypertrophy elicited by pathological stimuli eventually leads to cardiac dysfunction, exercise-induced hypertrophy does not. This sug-gests that a beneficial hypertrophic phenotype exists. In search of an under-lying molecular substrate we used microarray technology to identify cardiac gene expression in response to exercise. Rats exercised for seven weeks on a treadmill were characterized by invasive blood pressure mea-surements and echocardiography.

12p awards 06-04-2013 38 3   Download

New technologies enable acquisition of large data-sets containing genomic, proteomic and metabolic information that describe the state of a cell. These data-sets call for systematic methods enabling relevant information about the inner workings of the cell to be extracted. One important issue at hand is the understanding of the functional interactions between genes, proteins and metabolites.

11p awards 06-04-2013 29 2   Download

The reactive site loop of serpins undoubtedly defines in part their ability to inhibit a particular enzyme. Exchanges in the reactive loop of serpins might reassign the targets and modify the serpin–protease interaction kinetics. Based on this concept, we have developed a procedure to change the specificity of known serpins. First, reactive loops are very good substrates for the target enzymes. Therefore, we have used the phage-display technology to select from a penta-peptide phage library the best substrates for the human prostatekallikreinhK2 ...

7p dell39 03-04-2013 43 4   Download

The binding characteristics of a series of PPARcligands (GW9662, GI 262570, cis-parinaric acid, 15-deoxy-D 12,14 -prostaglandin J2 , LY171883, indomethacin, linoleic acid, palmitic acid and troglitazone) to human PPARcligand binding domain have been investigated for the first time by using surface plasmon resonance biosensor technology, CD spectroscopyandmoleculardockingsimulation.

12p dell39 03-04-2013 39 4   Download

For 30 years, the field of medical device technology has struggled to identify the specifications for optimal biocompatibility. For the vast majority of devices, clinical experience has eventually confirmed that the best performance is achieved when biocom-patibility is equated with biological safety that is materials are selected on the basis of maximizing bioinertness and minimizing toxicity and irritation.

7p inspiron33 26-03-2013 52 5   Download

The newly emerging field of systems biology involves a judicious interplay between high-throughput ‘wet’ experimentation, computational modelling and technology development, coupled to the world of ideas and theory. This interplay involves iterative cycles, such that systems biology is not at all confined to hypothesis-dependent studies, with intelligent, principled, hypothesis-generating studies being of high importance and consequently very far from aimless fishing expeditions.

22p inspiron33 26-03-2013 38 5   Download

We describe a platform that utilizes wheat germ cell-free technology to pro-duce protein samples for NMR structure determinations. In the first stage, cloned DNA molecules coding for proteins of interest are transcribed and translated on a small scale (25lL) to determine levels of protein expression and solubility.

10p inspiron33 25-03-2013 39 5   Download

A recent study that examined multiple strains ofCampylobacter jejuni reported that HS:19, a serostrain that has been associated with the onset of Guillain–Barre´ syndrome, had unidentified labile, capsular polysaccharide (CPS) structures. In this study, we expand on this observation by using current glyco-analytical technologies to characterize these unknown groups.

15p inspiron33 25-03-2013 38 4   Download